Literature DB >> 36173576

Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells.

Atsushi Kunii1, Takashi Yamamoto1, Tetsushi Sakuma2.   

Abstract

Genome editing technologies can be diverted into artificial transcription activators. In particular, researchers have improved dCas9-based technologies by tandem-fusing or trans-accumulating effector domains. Previously, we developed a hierarchical effector accumulation system named "TREE," enabling robust activation of target genes even when strongly silenced. In this chapter, we describe our protocol to design, construct, and validate the TREE-mediated target gene activation in cultured human cells.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  CRISPR–Cas9; Real-time PCR; Reporter assay; Transcriptional activation; dCas9; ddPCR

Mesh：

Substances：

Year:  2023        PMID： 36173576     DOI： 10.1007/978-1-0716-2724-2_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  17 in total

Review 1.  Various strategies of effector accumulation to improve the efficiency of genome editing and derivative methodologies.

Authors:  Atsushi Kunii; Takashi Yamamoto; Tetsushi Sakuma
Journal:  In Vitro Cell Dev Biol Anim       Date:  2020-06-08       Impact factor: 2.416

2.  Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.

Authors:  Y G Kim; J Cha; S Chandrasegaran
Journal:  Proc Natl Acad Sci U S A       Date:  1996-02-06       Impact factor: 11.205

3.  A TALE nuclease architecture for efficient genome editing.

Authors:  Jeffrey C Miller; Siyuan Tan; Guijuan Qiao; Kyle A Barlow; Jianbin Wang; Danny F Xia; Xiangdong Meng; David E Paschon; Elo Leung; Sarah J Hinkley; Gladys P Dulay; Kevin L Hua; Irina Ankoudinova; Gregory J Cost; Fyodor D Urnov; H Steve Zhang; Michael C Holmes; Lei Zhang; Philip D Gregory; Edward J Rebar
Journal:  Nat Biotechnol       Date:  2010-12-22       Impact factor: 54.908

4.  Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.

Authors:  Silvana Konermann; Mark D Brigham; Alexandro E Trevino; Julia Joung; Omar O Abudayyeh; Clea Barcena; Patrick D Hsu; Naomi Habib; Jonathan S Gootenberg; Hiroshi Nishimasu; Osamu Nureki; Feng Zhang
Journal:  Nature       Date:  2014-12-10       Impact factor: 49.962

5.  CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes.

Authors:  Luke A Gilbert; Matthew H Larson; Leonardo Morsut; Zairan Liu; Gloria A Brar; Sandra E Torres; Noam Stern-Ginossar; Onn Brandman; Evan H Whitehead; Jennifer A Doudna; Wendell A Lim; Jonathan S Weissman; Lei S Qi
Journal:  Cell       Date:  2013-07-11       Impact factor: 41.582

6.  Highly efficient Cas9-mediated transcriptional programming.

Authors:  Alejandro Chavez; Jonathan Scheiman; Suhani Vora; Benjamin W Pruitt; Marcelle Tuttle; Eswar P R Iyer; Shuailiang Lin; Samira Kiani; Christopher D Guzman; Daniel J Wiegand; Dmitry Ter-Ovanesyan; Jonathan L Braff; Noah Davidsohn; Benjamin E Housden; Norbert Perrimon; Ron Weiss; John Aach; James J Collins; George M Church
Journal:  Nat Methods       Date:  2015-03-02       Impact factor: 28.547

7.  A protein-tagging system for signal amplification in gene expression and fluorescence imaging.

Authors:  Marvin E Tanenbaum; Luke A Gilbert; Lei S Qi; Jonathan S Weissman; Ronald D Vale
Journal:  Cell       Date:  2014-10-09       Impact factor: 41.582

8.  Comparison of Cas9 activators in multiple species.

Authors:  Alejandro Chavez; Marcelle Tuttle; Benjamin W Pruitt; Ben Ewen-Campen; Raj Chari; Dmitry Ter-Ovanesyan; Sabina J Haque; Ryan J Cecchi; Emma J K Kowal; Joanna Buchthal; Benjamin E Housden; Norbert Perrimon; James J Collins; George Church
Journal:  Nat Methods       Date:  2016-05-23       Impact factor: 28.547

9.  Waking up dormant tumor suppressor genes with zinc fingers, TALEs and the CRISPR/dCas9 system.

Authors:  Benjamin Garcia-Bloj; Colette Moses; Agustin Sgro; Janice Plani-Lam; Mahira Arooj; Ciara Duffy; Shreyas Thiruvengadam; Anabel Sorolla; Rabab Rashwan; Ricardo L Mancera; Andrea Leisewitz; Theresa Swift-Scanlan; Alejandro H Corvalan; Pilar Blancafort
Journal:  Oncotarget       Date:  2016-09-13

10.  Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.

Authors:  Luke A Gilbert; Max A Horlbeck; Britt Adamson; Jacqueline E Villalta; Yuwen Chen; Evan H Whitehead; Carla Guimaraes; Barbara Panning; Hidde L Ploegh; Michael C Bassik; Lei S Qi; Martin Kampmann; Jonathan S Weissman
Journal:  Cell       Date:  2014-10-09       Impact factor: 41.582
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.