Simou Wu1, Lei Ren2, Jinxing Li1, Xi Shen1, Qingqing Zhou1, Zhonghua Miao1, Wen Jia1, Fang He3, Ruyue Cheng4. 1. Department of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, No. 16, 3rd Section, South Renmin Road, Wuhou District, Chengdu, 610041, Sichuan, People's Republic of China. 2. Hebei Inatural Bio-Tech Co.,Ltd., Shijiazhuang, 050000, Hebei, People's Republic of China. 3. Department of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, No. 16, 3rd Section, South Renmin Road, Wuhou District, Chengdu, 610041, Sichuan, People's Republic of China. hf18602880124@163.com. 4. Department of Nutrition and Food Hygiene, West China School of Public Health and West China Fourth Hospital, Sichuan University, No. 16, 3rd Section, South Renmin Road, Wuhou District, Chengdu, 610041, Sichuan, People's Republic of China. ruyuecheng1993@163.com.
Abstract
PURPOSE: This study was aimed to determine how delivery mode and feeding pattern influence the infant's gut microbiota construction and the variation of fecal microbial metabolites from a birth cohort. METHODS: Fecal samples collected from 61 full-term born Chinese infants at four time points: day 0, day 7, month 1, and month 3. Based on delivery mode (vaginal delivery [V] or cesarean section [C]) and feeding pattern (breastfeeding [B] or mixed feeding [M]), infants were divided into four groups, namely VB, CB, VM, and CM groups. The gut microbiota composition and bacterial diversity were assessed using 16S rRNA sequencing. Short-chain fatty acid (SCFA) concentrations were determined via gas chromatography-mass spectrometry (GC-MS). RESULTS: The CM group had a significantly higher relative abundance of Firmicutes (day 0 and month 1), Enterococcaceae (month 3), and Enterococcus (month 3) than the VB group and a significantly higher abundance of Firmicutes (month 1) and Blautia (month 3) than the CB group. The VB and CB groups exhibited a stable SCFA variation and a significantly lower level of propionate compared with the VM and CM groups. All groups showed an intense transition of enterotypes within 1 month and became stable at 3 months. The correlation between SCFA and enterotypes showed a significant positive correlation between Bifidobacteriaceae and acetate in the CB group (day 7 and month 3) and a significant positive correlation between Clostridiaceae and butyrate in the CB and VB groups (day 7 and month 3), respectively. CONCLUSION: These results indicated that C-section was associated with higher abundance of the phylum Firmicutes and family Enterococcaceae, and intense fluctuation of SCFA, at least propionate. And breastfeeding might partially contribute to gut microbiota construction and stabilization propionate metabolism in cesarean-section infants.
PURPOSE: This study was aimed to determine how delivery mode and feeding pattern influence the infant's gut microbiota construction and the variation of fecal microbial metabolites from a birth cohort. METHODS: Fecal samples collected from 61 full-term born Chinese infants at four time points: day 0, day 7, month 1, and month 3. Based on delivery mode (vaginal delivery [V] or cesarean section [C]) and feeding pattern (breastfeeding [B] or mixed feeding [M]), infants were divided into four groups, namely VB, CB, VM, and CM groups. The gut microbiota composition and bacterial diversity were assessed using 16S rRNA sequencing. Short-chain fatty acid (SCFA) concentrations were determined via gas chromatography-mass spectrometry (GC-MS). RESULTS: The CM group had a significantly higher relative abundance of Firmicutes (day 0 and month 1), Enterococcaceae (month 3), and Enterococcus (month 3) than the VB group and a significantly higher abundance of Firmicutes (month 1) and Blautia (month 3) than the CB group. The VB and CB groups exhibited a stable SCFA variation and a significantly lower level of propionate compared with the VM and CM groups. All groups showed an intense transition of enterotypes within 1 month and became stable at 3 months. The correlation between SCFA and enterotypes showed a significant positive correlation between Bifidobacteriaceae and acetate in the CB group (day 7 and month 3) and a significant positive correlation between Clostridiaceae and butyrate in the CB and VB groups (day 7 and month 3), respectively. CONCLUSION: These results indicated that C-section was associated with higher abundance of the phylum Firmicutes and family Enterococcaceae, and intense fluctuation of SCFA, at least propionate. And breastfeeding might partially contribute to gut microbiota construction and stabilization propionate metabolism in cesarean-section infants.
Authors: Khanh Vu; Wendy Lou; Hein M Tun; Theodore B Konya; Nadia Morales-Lizcano; Radha S Chari; Catherine J Field; David S Guttman; Rupasri Mandal; David S Wishart; Meghan B Azad; Allan B Becker; Piush J Mandhane; Theo J Moraes; Diana L Lefebvre; Malcolm R Sears; Stuart E Turvey; Padmaja Subbarao; James A Scott; Anita L Kozyrskyj Journal: Gastroenterology Date: 2020-09-16 Impact factor: 22.682
Authors: Pia S Pannaraj; Fan Li; Chiara Cerini; Jeffrey M Bender; Shangxin Yang; Adrienne Rollie; Helty Adisetiyo; Sara Zabih; Pamela J Lincez; Kyle Bittinger; Aubrey Bailey; Frederic D Bushman; John W Sleasman; Grace M Aldrovandi Journal: JAMA Pediatr Date: 2017-07-01 Impact factor: 16.193
Authors: Nicholas A Bokulich; Jennifer Chung; Thomas Battaglia; Nora Henderson; Melanie Jay; Huilin Li; Arnon D Lieber; Fen Wu; Guillermo I Perez-Perez; Yu Chen; William Schweizer; Xuhui Zheng; Monica Contreras; Maria Gloria Dominguez-Bello; Martin J Blaser Journal: Sci Transl Med Date: 2016-06-15 Impact factor: 17.956
Authors: Helena M Hesla; Fredrik Stenius; Lotta Jäderlund; Ronald Nelson; Lars Engstrand; Johan Alm; Johan Dicksved Journal: FEMS Microbiol Ecol Date: 2014-11-03 Impact factor: 4.194