Literature DB >> 36151502

Protein Crystallization of Two Recombinant Lpt Proteins.

Michela Bollati1,2, Louise J Gourlay3.   

Abstract

The prerequisite for 3D structure determination of macromolecules via X-ray crystallography is well-ordered, diffracting crystals. Here, we report the recombinant production, biophysical/biochemical protein sample characterization, and vapor diffusion sitting drop crystallization protocols for two lipopolysaccharide transport proteins: LptH from Pseudomonas aeruginosa (Pa-LptH) and an inactive LptC mutant (G153R) from Escherichia coli (EcLptC24-191G153R).
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  E. coli; Lipopolysaccharide transport protein; Outer membrane biogenesis; Protein crystallization; Vapor diffusion

Mesh：

Substances：

Year:  2022        PMID： 36151502     DOI： 10.1007/978-1-0716-2581-1_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  The Protein Data Bank.

Authors:  H M Berman; J Westbrook; Z Feng; G Gilliland; T N Bhat; H Weissig; I N Shindyalov; P E Bourne
Journal:  Nucleic Acids Res       Date:  2000-01-01       Impact factor: 16.971

2.  XtalPred: a web server for prediction of protein crystallizability.

Authors:  Lukasz Slabinski; Lukasz Jaroszewski; Leszek Rychlewski; Ian A Wilson; Scott A Lesley; Adam Godzik
Journal:  Bioinformatics       Date:  2007-10-05       Impact factor: 6.937

3.  Control of transcription termination by an RNA factor in bacteriophage P4 immunity: identification of the target sites.

Authors:  P Sabbattini; F Forti; D Ghisotti; G Dehò
Journal:  J Bacteriol       Date:  1995-03       Impact factor: 3.490

4.  New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex.

Authors:  Paola Sperandeo; Riccardo Villa; Alessandra M Martorana; Maria Samalikova; Rita Grandori; Gianni Dehò; Alessandra Polissi
Journal:  J Bacteriol       Date:  2010-12-17       Impact factor: 3.490

Review 5.  An overview of biological macromolecule crystallization.

Authors:  Irene Russo Krauss; Antonello Merlino; Alessandro Vergara; Filomena Sica
Journal:  Int J Mol Sci       Date:  2013-05-31       Impact factor: 5.923
  5 in total

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