| Literature DB >> 36149539 |
Sara Bellali1, Gabriel Haddad1,2, Thi-Phuong-Thao Pham1, Rim Iwaza1,2, Ahmad Ibrahim1,2, Nicholas Armstrong1, Amael Fadlane1, Carine Couderc1, Aldiouma Diallo3, Cheikh Sokhna3,4, Matthieu Million1, Didier Raoult1,2, Maryam Tidjani Alou5.
Abstract
Two strains, designated as Marseille-P2918T and Marseille-P3646T, were isolated from a 14-week-old Senegalese girl using culturomics: Urmitella timonensis strain Marseille-P2918T (= CSUR P2918, = DSM 103634) and Marasmitruncus massiliensis strain Marseille-P3646T (= CSUR P3646, = CCUG72353). Both strains were rod-shaped, anaerobic, spore forming motile bacteria. The 16S rRNA gene sequences of strains Marseille-P2918T (LT598554) and Marseille-P3646T (LT725660) shared 93.25% and 94.34% identity with Tissierella praeacuta ATCC 25539T and Anaerotruncus colihominis CIP 107754T, their respective phylogenetically closest species with standing in nomenclature. Therefore, strain Marseille-P2918T is classified within the family Tissierellaceae and order Tissierellales whereas strain Marseille-P3646T is classified within the family Oscillospiraceae and order Eubacteriales. The genome of strain Marseille-P2918T had a size of 2.13 Mb with a GC content of 50.52% and includes six scaffolds and six contigs, and that of strain Marseille-P3646T was 3.76 Mbp long consisting of five contigs with a 50.04% GC content. The genomes of both strains presented a high percentage of genes encoding enzymes involved in genetic information and processing, suggesting a high growth rate and adaptability. These new taxa are extensively described and characterised in this paper, using the concept of taxono-genomic description.Entities:
Keywords: Culturomics; Human gut microbiota; Marasmitruncus massiliensis; New taxa; Taxono-genomics; Urmitella timonensis
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Year: 2022 PMID： 36149539 PMCID： PMC9584879 DOI： 10.1007/s10482-022-01777-x
Source DB: PubMed Journal: Antonie Van Leeuwenhoek ISSN： 0003-6072 Impact factor: 2.158