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Literature DB >> 36107341

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization.

Nora Lisa Bitzenhofer¹, Fabienne Hilgers¹, Gabriela N Bosio², Joaquim Torra³, Giorgia Casini², Felix R M Beinlich², Esther Knieps-Grünhagen¹, Valentin Gordeliy^4,5, Karl-Erich Jaeger^1,2, Santi Nonell⁶, Ulrich Krauss^7,8, Thomas Gensch⁹, Thomas Drepper¹⁰.

Abstract

Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.

Entities: Chemical

Keywords: Flavin; Flavin-binding fluorescent protein; Fluorescence brightness; Fluorescence lifetime; Fluorescence microscopy; Genetically encoded photosensitizer; LOV domain; Phototoxicity

Mesh：

Substances：

Year: 2023 PMID： 36107341 DOI： 10.1007/978-1-0716-2667-2_7

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

37 in total

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