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Literature DB >> 3610155

Determination of the serological sex-specific (Sxs) antigen ("H-Y antigen") in birds and mammals using high-titer antisera and a sensitive urease ELISA.

Abstract

A rapid, sensitive, and reproducible enzyme-linked immunosorbent assay (ELISA), for the detection of the serological sex-specific (Sxs) antigen (formerly termed H-Y antigen; see Introduction), is described. This assay uses bovine testes extract as the solid phase antigen, and high-titer anti-Sxs antisera and a urease-conjugated anti rat-IgG as the first and second antibody respectively. The urea containing substrate causes a pH shift in a positive reaction, which in turn is visualized by the use of bromocresol purple as a pH indicator. The method, and some representative applications of it, are described in detail.

Entities: Chemical Species

Mesh：

Substances：
H-Y Antigen

Year: 1987 PMID： 3610155 DOI： 10.1007/bf00272444

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

24 in total

1. Skin.

Authors: E J Eichwald; C R Silmser
Journal: Transplant Bull Date: 1955

2. The testis as a secretory organ for H-Y antigen.

Authors: U Müller; J W Siebers; M T Zenzes; U Wolf
Journal: Hum Genet Date: 1978-12-18 Impact factor: 4.132

3. Serological demonstration of H-Y (male) antigen on mouse sperm.

Authors: E H Goldberg; E A Boyse; D Bennett; M Scheid; E A Carswell
Journal: Nature Date: 1971-08-13 Impact factor: 49.962

4. Elicitation of a rapid and transient antibody response to H-Y antigen by intrasplenic immunization.

Authors: M P Bradley; B F Heslop
Journal: Transplantation Date: 1985-06 Impact factor: 4.939

Review 5. The Y-linked testis determining gene and H-Y plasma membrane antigen gene: are they one and the same?

Authors: S Ohno
Journal: Endocr Rev Date: 1985 Impact factor: 19.871

6. Studies on H-Y antigen in different cell fractions of the testis during pubescence: immature germ cells are H-Y antigen negative.

Authors: M T Zenzes; U Müller; I Aschmoneit; U Wolf
Journal: Hum Genet Date: 1978-12-29 Impact factor: 4.132

7. Turner syndrome patients are H-Y positive.

Authors: U Wolf; M Fraccaro; A Mayerová; T Hecht; O Zuffardi; H Hameister
Journal: Hum Genet Date: 1980 Impact factor: 4.132

8. On the secretion of H-Y antigen.

Authors: M Brunner; C A Moreira-Filho; G Wachtel; S Wachtel
Journal: Cell Date: 1984-06 Impact factor: 41.582

9. Serological crossreactivity between H-Y (male) antigens of mouse and man.

Authors: S S Wachtel; G C Koo; E E Zuckerman; U Hammerling; M P Scheid; E A Boyse
Journal: Proc Natl Acad Sci U S A Date: 1974-04 Impact factor: 11.205

10. Association of the H-Y male antigen with beta2-microglobulin on human lymphoid and differentiated mouse teratocarcinoma cell lines.

Authors: M Fellous; E Günther; R Kemler; J Wiels; R Berger; J L Guenet; H Jakob; F Jacob
Journal: J Exp Med Date: 1978-07-01 Impact factor: 14.307

6 in total

6. The distribution of sex-specific (H-Y) antigens within the seminiferous tubules of the testis: an immunohistochemical study.

Authors: M P Bradley; B F Heslop
Journal: Hum Genet Date: 1988-08 Impact factor: 4.132