Teruki Nii1,2, Shunsuke Takizawa3, Tomomi Akita3,4, Chikamasa Yamashita3,4, Issei Takeuchi5,4,6, Kimiko Makino3,4. 1. Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan; nii.teruki.204@m.kyushu-u.ac.jp takeuchi@rs.tus.ac.jp. 2. Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan. 3. Faculty of Pharmaceutical Sciences, Tokyo University of Sciences, Yamazaki, Japan. 4. Center for Drug Delivery Research, Tokyo University of Sciences, Yamazaki, Japan. 5. Faculty of Pharmaceutical Sciences, Tokyo University of Sciences, Yamazaki, Japan; nii.teruki.204@m.kyushu-u.ac.jp takeuchi@rs.tus.ac.jp. 6. Josai International University, Togane, Japan.
Abstract
BACKGROUND/AIM: In vivo models of tuberculosis are effective tools for developing new drugs. The objective of this study was to prepare in vivo models for tuberculosis by utilizing nanocomposite particles (NCPs) containing imiquimod-loaded poly(lactic-co-glycolic acid) nanoparticles. MATERIALS AND METHODS: NCPs were prepared from dichloromethane with imiquimod and poly(lactic-co-glycolic acid) using a spray dryer. Mice were treated with NCPs in the lungs by inhalation, and then infection with Mycobacterium bovis bacille Calmette-Guerin was performed (treatment groups). The concentrations of the pro-inflammatory cytokines, tumor necrosis factor-α and interferon-γ were measured in bronchoalveolar lavage fluid using an enzyme-linked immunosorbent assay. RESULTS: When animals were treated with NCPs, the concentrations of tumor necrosis factor-α and interferon-γ in bronchoalveolar lavage fluid were significantly higher than in animals not treated with NCPs. In addition, high bacterial counts and circular granuloma were observed. CONCLUSION: NCPs prepared in this study enhanced the level of inflammation in the lungs and support the preparation of in vivo models of tuberculosis.
BACKGROUND/AIM: In vivo models of tuberculosis are effective tools for developing new drugs. The objective of this study was to prepare in vivo models for tuberculosis by utilizing nanocomposite particles (NCPs) containing imiquimod-loaded poly(lactic-co-glycolic acid) nanoparticles. MATERIALS AND METHODS: NCPs were prepared from dichloromethane with imiquimod and poly(lactic-co-glycolic acid) using a spray dryer. Mice were treated with NCPs in the lungs by inhalation, and then infection with Mycobacterium bovis bacille Calmette-Guerin was performed (treatment groups). The concentrations of the pro-inflammatory cytokines, tumor necrosis factor-α and interferon-γ were measured in bronchoalveolar lavage fluid using an enzyme-linked immunosorbent assay. RESULTS: When animals were treated with NCPs, the concentrations of tumor necrosis factor-α and interferon-γ in bronchoalveolar lavage fluid were significantly higher than in animals not treated with NCPs. In addition, high bacterial counts and circular granuloma were observed. CONCLUSION: NCPs prepared in this study enhanced the level of inflammation in the lungs and support the preparation of in vivo models of tuberculosis.
Authors: Dimitry A Chistiakov; Veronika A Myasoedova; Victor V Revin; Alexander N Orekhov; Yuri V Bobryshev Journal: Immunobiology Date: 2017-10-05 Impact factor: 3.144