Ana Lozano-Guillén1, Sergio López-García2,3, Francisco Javier Rodríguez-Lozano2,3, José Luis Sanz4, Adrián Lozano1, Carmen Llena1, Leopoldo Forner1. 1. Department of Stomatology, Faculty of Medicine and Dentistry, Universitat de València, 46010, Valencia, Spain. 2. Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria Virgen de La Arrixaca, IMIB-Arrixaca, University of Murcia, 30120, Murcia, Spain. 3. Department of Dermatology, Stomatology, Radiology and Physical Medicine, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, 30008, Murcia, Spain. 4. Department of Stomatology, Faculty of Medicine and Dentistry, Universitat de València, 46010, Valencia, Spain. Jose.L.Sanz@uv.es.
Abstract
OBJECTIVES: The aim of the present in vitro study is to determine the cytocompatibility of the recently introduced NeoPutty in contact with human dental pulp cells compared with its precursor NeoMTA Plus and the classic gold standard MTA Angelus. MATERIALS AND METHODS: Sample disks were obtained for each of the tested materials (5 mm diameter; 2 mm thickness; n = 30), along with 1:1, 1:2, and 1:4 material eluents. HDPCs were extracted and cultured with the tested materials (test groups) or in unconditioned medium (control group), and the following biocompatibility assays were performed: MTT assay, scratch wound assay, cell cytoskeleton staining assays, and cell attachment assessment via SEM. Additionally, material ion release and surface element composition were evaluated via ICP-MS and SEM-EDX, respectively. Each experimental condition was carried out three times and assessed in three independent experiments. Statistical significance was established at p < 0.05. RESULTS: 1:2 dilutions of all the tested materials exhibited a comparable cell viability to that of the control group at 48 and 72 h of culture (p < 0.05). The same was observed for 1:4 dilutions of the tested materials at 24, 48, and 72 h of culture (p > 0.05). All the tested materials exhibited adequate cytocompatibility in the remaining biocompatibility assays. MTA exhibited a significantly higher calcium ion release compared to NeoPutty and NeoMTA Plus (p < 0.05). CONCLUSION: The results from the present work elucidate the adequate cytocompatibility of NeoPutty, NeoMTA Plus, and MTA Angelus towards human dental pulp cells. CLINICAL RELEVANCE: Within the limitations of the present in vitro study, our results may act as preliminary evidence for its use in vital pulp therapy as a pulp capper. However, results need to be interpreted with caution until further clinical supporting evidence is reported.
OBJECTIVES: The aim of the present in vitro study is to determine the cytocompatibility of the recently introduced NeoPutty in contact with human dental pulp cells compared with its precursor NeoMTA Plus and the classic gold standard MTA Angelus. MATERIALS AND METHODS: Sample disks were obtained for each of the tested materials (5 mm diameter; 2 mm thickness; n = 30), along with 1:1, 1:2, and 1:4 material eluents. HDPCs were extracted and cultured with the tested materials (test groups) or in unconditioned medium (control group), and the following biocompatibility assays were performed: MTT assay, scratch wound assay, cell cytoskeleton staining assays, and cell attachment assessment via SEM. Additionally, material ion release and surface element composition were evaluated via ICP-MS and SEM-EDX, respectively. Each experimental condition was carried out three times and assessed in three independent experiments. Statistical significance was established at p < 0.05. RESULTS: 1:2 dilutions of all the tested materials exhibited a comparable cell viability to that of the control group at 48 and 72 h of culture (p < 0.05). The same was observed for 1:4 dilutions of the tested materials at 24, 48, and 72 h of culture (p > 0.05). All the tested materials exhibited adequate cytocompatibility in the remaining biocompatibility assays. MTA exhibited a significantly higher calcium ion release compared to NeoPutty and NeoMTA Plus (p < 0.05). CONCLUSION: The results from the present work elucidate the adequate cytocompatibility of NeoPutty, NeoMTA Plus, and MTA Angelus towards human dental pulp cells. CLINICAL RELEVANCE: Within the limitations of the present in vitro study, our results may act as preliminary evidence for its use in vital pulp therapy as a pulp capper. However, results need to be interpreted with caution until further clinical supporting evidence is reported.
Authors: Mar Collado-González; Miguel R Pecci-Lloret; Christopher J Tomás-Catalá; David García-Bernal; Ricardo E Oñate-Sánchez; Carmen Llena; Leopoldo Forner; Vinicius Rosa; Francisco J Rodríguez-Lozano Journal: Dent Mater Date: 2018-04-09 Impact factor: 5.304
Authors: Christopher J Tomás-Catalá; Mar Collado-González; David García-Bernal; Ricardo E Oñate-Sánchez; Leopoldo Forner; Carmen Llena; Adrián Lozano; José M Moraleda; Francisco J Rodríguez-Lozano Journal: J Endod Date: 2017-11-01 Impact factor: 4.171
Authors: F J Rodríguez-Lozano; A Lozano; S López-García; D García-Bernal; J L Sanz; J Guerrero-Gironés; C Llena; L Forner; M Melo Journal: Clin Oral Investig Date: 2021-08-12 Impact factor: 3.573