Mar Collado-González1, Miguel R Pecci-Lloret2, Christopher J Tomás-Catalá3, David García-Bernal1, Ricardo E Oñate-Sánchez2, Carmen Llena4, Leopoldo Forner4, Vinicius Rosa5, Francisco J Rodríguez-Lozano6. 1. Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain. 2. School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain. 3. Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain; School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain. 4. Department of Stomatology, University of Valencia, Valencia, Spain. 5. Faculty of Dentistry, National University of Singapore, Singapore, Singapore. 6. Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain; School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain. Electronic address: fcojavier@um.es.
Abstract
OBJECTIVE: To evaluate the in vitro cytotoxicity of Equia Forte (GC, Tokyo, Japan) and Ionostar Molar (Voco, Cuxhaven, Germany) on human dental pulp stem cells (hDPSCs). METHODS: hDPSCs isolated from third molars were exposed to several dilutions of Equia Forte and Ionostar Molar eluates (1/1, 1/2 and 1/4). These eluates were obtained by storing material samples in respective cell culture medium for 24h (n=40). hDPSCs in basal growth culture medium were the control. Cell viability and cell migration assays were performed using the MTT and wound-healing assays, respectively. Also, induction of apoptosis and changes in cell phenotype were evaluated by flow cytometry. Changes in cell morphology were analysed by immunocytofluorescence staining. To evaluate cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was determined by energy dispersive X-ray (EDX) and eluates were analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with analysis of variance (ANOVA) and Student's t-test (α<0.05). RESULTS: Undiluted Equia Forte extracts led to a similar cell proliferation rates than the control group from 72h onwards. There were no significance differences between Equia Forte and Ionostar Molar in terms of cell apoptosis and phenotype. However, in presence of Equia extracts the migration capacity of hDPSCs was higher than in presence of Ionostar Molar (p<0.05). Also, SEM studies showed a higher degree of cell attachment when Equia Forte extracts were used. Finally, EDX analysis pointed to different weight percentages of C, O and Ca ions in glass ionomer cements, while other elements such as La, Al, Si, W, Mo and F were also detected. SIGNIFICANCE: In summary, Equia Forte promoted better biological responses in hDPSCs than Ionostar Molar.
OBJECTIVE: To evaluate the in vitro cytotoxicity of Equia Forte (GC, Tokyo, Japan) and Ionostar Molar (Voco, Cuxhaven, Germany) on human dental pulp stem cells (hDPSCs). METHODS: hDPSCs isolated from third molars were exposed to several dilutions of Equia Forte and Ionostar Molar eluates (1/1, 1/2 and 1/4). These eluates were obtained by storing material samples in respective cell culture medium for 24h (n=40). hDPSCs in basal growth culture medium were the control. Cell viability and cell migration assays were performed using the MTT and wound-healing assays, respectively. Also, induction of apoptosis and changes in cell phenotype were evaluated by flow cytometry. Changes in cell morphology were analysed by immunocytofluorescence staining. To evaluate cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy (SEM). The chemical composition of the materials was determined by energy dispersive X-ray (EDX) and eluates were analyzed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with analysis of variance (ANOVA) and Student's t-test (α<0.05). RESULTS: Undiluted Equia Forte extracts led to a similar cell proliferation rates than the control group from 72h onwards. There were no significance differences between Equia Forte and Ionostar Molar in terms of cell apoptosis and phenotype. However, in presence of Equia extracts the migration capacity of hDPSCs was higher than in presence of Ionostar Molar (p<0.05). Also, SEM studies showed a higher degree of cell attachment when Equia Forte extracts were used. Finally, EDX analysis pointed to different weight percentages of C, O and Ca ions in glass ionomer cements, while other elements such as La, Al, Si, W, Mo and F were also detected. SIGNIFICANCE: In summary, Equia Forte promoted better biological responses in hDPSCs than Ionostar Molar.
Authors: Ana Lozano-Guillén; Sergio López-García; Francisco Javier Rodríguez-Lozano; José Luis Sanz; Adrián Lozano; Carmen Llena; Leopoldo Forner Journal: Clin Oral Investig Date: 2022-09-02 Impact factor: 3.606
Authors: Carmen Llena; Mar Collado-González; Christopher Joseph Tomás-Catalá; David García-Bernal; Ricardo Elías Oñate-Sánchez; Francisco Javier Rodríguez-Lozano; Leopoldo Forner Journal: Materials (Basel) Date: 2018-06-27 Impact factor: 3.623
Authors: Fernanda M Tsuzuki; Renata C Pascotto; Luis C Malacarne; Antonio C Bento; Antonio Medina Neto; Lidiane Vizioli de Castro-Hoshino; Monique Souza; John W Nicholson; Mauro L Baesso Journal: Biomater Investig Dent Date: 2021-04-01
Authors: James Ghilotti; José Luis Sanz; Sergio López-García; Julia Guerrero-Gironés; María P Pecci-Lloret; Adrián Lozano; Carmen Llena; Francisco Javier Rodríguez-Lozano; Leopoldo Forner; Gianrico Spagnuolo Journal: Materials (Basel) Date: 2020-05-10 Impact factor: 3.623
Authors: Sergio López-García; María P Pecci-Lloret; Miguel R Pecci-Lloret; Ricardo E Oñate-Sánchez; David García-Bernal; Pablo Castelo-Baz; Francisco Javier Rodríguez-Lozano; Julia Guerrero-Gironés Journal: Materials (Basel) Date: 2019-11-08 Impact factor: 3.623
Authors: F J Rodríguez-Lozano; A Lozano; S López-García; D García-Bernal; J L Sanz; J Guerrero-Gironés; C Llena; L Forner; M Melo Journal: Clin Oral Investig Date: 2021-08-12 Impact factor: 3.573