Christopher K Cote1, Jessica M Weidner2, Christopher Klimko1, Ashley E Piper3, Jeremy A Miller4, Melissa Hunter1, Jennifer L Shoe1, Jennifer C Hoover3, Brian R Sauerbry5, Tony Buhr6, Joel A Bozue1, David E Harbourt4, Pamela J Glass3. 1. Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. 2. Medical Translational Sciences Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. 3. Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. 4. Biosafety Office, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. 5. Logistics Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. 6. Naval Surface Warfare Center, Dahlgren Division, Dahlgren, VA, USA.
Abstract
Introduction: Failure of an existing effluent decontamination system (EDS) prompted the consideration of commercial off-the-shelf solutions for decontamination of containment laboratory waste. A bleach-based chemical EDS was purchased to serve as an interim solution. Methods: Studies were conducted in the laboratory to validate inactivation of Bacillus spores with bleach in complex matrices containing organic simulants including fetal bovine serum, humic acid, and animal room sanitation effluent. Results: These studies demonstrated effective decontamination of >106 spores at a free chlorine concentration of ≥5700 parts per million with a 2-hour contact time. Translation of these results to biological validation of the bleach-based chemical EDS required some modifications to the system and its operation. Discussion: The chemical EDS was validated for the treatment of biosafety levels 3 and 4 waste effluent using laboratory-prepared spore packets along with commercial biological indicators; however, several issues and lessons learned identified during the process of onboarding are also discussed, including bleach product source, method of validation, dechlorination, and treated waste disposal. Copyright 2021, ABSA International 2021.
Introduction: Failure of an existing effluent decontamination system (EDS) prompted the consideration of commercial off-the-shelf solutions for decontamination of containment laboratory waste. A bleach-based chemical EDS was purchased to serve as an interim solution. Methods: Studies were conducted in the laboratory to validate inactivation of Bacillus spores with bleach in complex matrices containing organic simulants including fetal bovine serum, humic acid, and animal room sanitation effluent. Results: These studies demonstrated effective decontamination of >106 spores at a free chlorine concentration of ≥5700 parts per million with a 2-hour contact time. Translation of these results to biological validation of the bleach-based chemical EDS required some modifications to the system and its operation. Discussion: The chemical EDS was validated for the treatment of biosafety levels 3 and 4 waste effluent using laboratory-prepared spore packets along with commercial biological indicators; however, several issues and lessons learned identified during the process of onboarding are also discussed, including bleach product source, method of validation, dechlorination, and treated waste disposal. Copyright 2021, ABSA International 2021.
Entities:
Keywords:
Bacillus; biological validation; bleach; chemical effluent decontamination system; spore
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