| Literature DB >> 36008676 |
Michael D Kruppa1, Douglas W Lowman2, Harry E Ensley2, Zuchao Ma2, Bridget Graves3, Jennifer Kintner4, Jennifer V Hall5, Tammy R Ozment2, David L Williams2.
Abstract
The cell wall contains mannans and glucans that are recognized by the host immune system. In this chapter, we will describe the methods to isolate mannans and glucans from the C. albicans cell wall. In addition, we describe how to determine purity, molecular size, and structure of the mannans and glucans. We also detail how to prepare the carbohydrates for in vitro, ex vivo, or in vivo use by describing endotoxin removal (depyrogenation), derivatization, and labeling and evaluation of bioactivity.Entities:
Keywords: Bio-Layer Interferometry; Dectin-1; Dectin-2; Gel permeation chromatography; Glucan; Mannan; Mannose receptor; NMR
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Year: 2022 PMID： 36008676 DOI： 10.1007/978-1-0716-2549-1_24
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745