| Literature DB >> 35985322 |
Zhuohe Liu1, Xiaoyu Lu2, Vincent Villette3, Yueyang Gou4, Kevin L Colbert4, Shujuan Lai4, Sihui Guan4, Michelle A Land4, Jihwan Lee5, Tensae Assefa6, Daniel R Zollinger4, Maria M Korympidou7, Anna L Vlasits8, Michelle M Pang9, Sharon Su9, Changjia Cai10, Emmanouil Froudarakis11, Na Zhou4, Saumil S Patel4, Cameron L Smith12, Annick Ayon3, Pierre Bizouard3, Jonathan Bradley3, Katrin Franke7, Thomas R Clandinin9, Andrea Giovannucci13, Andreas S Tolias14, Jacob Reimer12, Stéphane Dieudonné3, François St-Pierre15.
Abstract
Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method of choice for deep-tissue recording. To improve indicators, we developed a multiparameter high-throughput platform to optimize voltage indicators for two-photon microscopy. Using this system, we identified JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can report light-evoked responses in axonal termini of Drosophila interneurons and the dendrites and somata of amacrine cells of isolated mouse retina. JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy. Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons.Entities:
Keywords: GEVI; JEDI-2P; fly vision; genetically encoded voltage indicator; high-throughput screening; pairwise voltage correlations; random-access microscopy; starburst amacrine cells; two-photon fluorescence microscopy; voltage imaging
Mesh：
Year: 2022 PMID： 35985322 PMCID： PMC9563101 DOI： 10.1016/j.cell.2022.07.013
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 66.850