| Literature DB >> 35980572 |
Orsolya Fölsz1, Chun-Chieh Lin2, Darya Task3, Olena Riabinina1, Christopher J Potter4.
Abstract
Binary expression systems are useful genetic tools for experimentally labeling or manipulating the function of defined cells. The Q-system is a repressible binary expression system that consists of a transcription factor QF (and the recently improved QF2/QF2w), the inhibitor QS, a QUAS-geneX effector, and a drug that inhibits QS (quinic acid). The Q-system can be used alone or in combination with other binary expression systems, such as GAL4/UAS and LexA/LexAop. In this review chapter, we discuss the past, present, and future of the Q-system for applications in Drosophila and other organisms. We discuss the in vivo application of the Q-system for transgenic labeling, the modular nature of QF that allows chimeric or split transcriptional activators to be developed, its temporal control by quinic acid, new methods to generate QF2 reagents, intersectional expression labeling, and its recent adoption into many emerging experimental species.Entities:
Keywords: Bioengineering; Chimeric transactivators; Drosophila; HACK; Mosquito; Neurospora crassa; Split-QF; Synthetic biology
Mesh：
Substances：
Year: 2022 PMID： 35980572 DOI： 10.1007/978-1-0716-2541-5_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745