Association of Time in Range with Endothelial Injury in Patients with Type 2 Diabetes Treated with Exenatide.

INTRODUCTION: We aimed to investigate whether treatment with exenatide could increase time in range (TIR) and decrease glycemic variability, and to evaluate the association between TIR and endothelial injury in patients with type 2 diabetes mellitus (T2DM).
METHODS: Two-hundred patients with T2DM treated with exenatide for 16 weeks were included in this study. Seven-point fingerstick blood glucose was used to evaluate derived TIR and glycemic variability. The serum levels of soluble endothelial cell protein C receptor (sEPCR) and von Willebrand factor (vWF) were measured. Ninety-three patients having the data of endothelial injury markers were categorized as derived TIR > 70% or ≤ 70% after the treatment and the association between TIR and endothelial injury were evaluated.
RESULTS: Treatment with exenatide for 16 weeks resulted in a significant reduction in fasting blood glucose, postprandial 2 h blood glucose, and glycated hemoglobin A1c (HbA1c) levels in patients with T2DM. Compared with baseline, derived TIR value was significantly increased [85.7 (57.1, 100.0) % vs. 42.9 (14.9, 71.4) %, P < 0.001], and the parameters of glycemic variability were remarkably decreased after the treatment. After the treatment, serum sEPCR level was significantly decreased from baseline in patients with TIR > 70% [74.5 (32.8, 122.5) ng/mL vs. 96.9 (48.5, 150.9) ng/mL, P = 0.006] but not in those with TIR ≤ 70%; serum vWF level was remarkably decreased in patients with TIR > 70% [from 1166.2 (848.1, 1335.5) mIU/mL to 907.4 (674.3, 1335.1) mIU/mL, P = 0.001] while this effect was modest in those with TIR ≤ 70%.
CONCLUSIONS: Treatment with exenatide increases TIR and decreases glycemic variability in patients with T2DM. Moreover, the amelioration of endothelial injury is more pronounced in patients with TIR > 70% after the treatment. TRIAL REGISTRATION: ChiCTR-IPR-15006558 (registered, 27 May 2015).

Key Summary Points

Introduction

The increasing prevalence and incidence of diabetes and its complications represent a global public health burden. According to data released by the International Diabetes Federation, there are 537 million diabetic adults aged 20–79 years old in 2021, and this number is expected to reach 783 million in 2045 [1]. Intensive glycemic control can decrease the incidence of diabetic vascular complications and reduce all-cause mortality [2]. Although glycated hemoglobin A1c (HbA1c) is the gold standard for monitoring glycemic control at 2–3 months prior to the measurement, it provides no information of glycemic variability, which can exert deleterious effects on the endothelium and lead to diabetic vascular complications in type 2 diabetes mellitus (T2DM) [3, 4]. Time in range (TIR) refers to the percentage of time that glucose is within the target range (usually 3.9–10.0 mmol/L in patients with type 1 diabetes mellitus and T2DM) per day, and is recommended as a parameter of glycemic control that provides valuable information beyond HbA1c alone [5]. The increased TIR is associated with lower incidence of diabetic macrovascular and microvascular complications, making TIR to become a key metric of glycemic control in clinical practice [6, 7].

As the most important complications of diabetes, vascular complications are the major cause of death and disability in patients with diabetes, and vascular endothelial injury is their primary pathogenesis [8, 9]. Therefore, in the treatment of diabetes, glucose-lowering agents are expected to prevent and/or treat diabetic vascular complications beyond their glucose-lowering effect. Glucagon-like peptide-1 receptor agonists (GLP-1RAs), a class of glucose-lowering agents, which can enhance glucose-dependent insulin secretion, inhibit glucagon secretion, suppress appetite, and delay gastric emptying, are widely used in the treatment of patients with T2DM [10]. Data from cardiovascular outcome trials show that GLP-1RAs have cardiovascular protective effects. Moreover, meta-analysis indicates that GLP-1RAs also possess potential effects against diabetic microvascular complications [11, 12]. However, whether increment of TIR is associated with improvement of vascular endothelial dysfunction in patients with type 2 diabetes treated with GLP-1RAs remains to be clarified.

In the present study, we aimed to investigate whether treatment with exenatide, the first GLP-1RA approved for the treatment of T2DM in China in 2009, could increase TIR and decrease glycemic variability, and to identify the association between TIR and endothelial injury markers in patients with T2DM. Our study supplies evidence for the diabetes treatment and helps precise diabetes management.

Methods

Study Design and Participants

The participants in this study were from a randomized, multicenter, non-inferiority clinical trial. We enrolled patients with T2DM aged 20–70 years, with the HbA1c levels ranging from 7.0% to 10.0%. All patients included in the trial had received monotherapy or combination treatment with metformin and insulin secretagogues. Patients were randomized to receive branded exenatide (Byetta®, Lilly Pharma Fertigung und Distribution GmbH & Co. KG, Giessen, Germany) or generic exenatide (Qinghai Chenfei Pharmaceutical Co. Ltd, Qinghai, China). In both groups, exenatide was administered subcutaneously at an initial dose of 5 μg twice daily for 4 weeks, followed by 10 μg twice daily for an additional 12 weeks (keeping the primary dosage if patients were unable to tolerate the increase). Full details of study design and protocol have been published elsewhere [13]. The trial was registered at www.chictr.org.cn (ChiCTR-IPR-15006558). A total of 240 patients were treated with either of the exenatides in this study. Forty patients were excluded because of protocol violation, loss to follow-up, withdrawal due to adverse events, refusal to continue participation, or missing baseline data for derived TIR calculation. Therefore, 200 patients were included in the final analyses (Supplementary Fig. 1).

This study was approved by the Ethics Committee of Peking University Third Hospital and conducted in accordance with the ethical principles of the Declaration of Helsinki of 1964 and its later amendments. All patients signed written informed consent before enrollment.

Blood Collection and Measurement

Blood samples were collected from each patient at baseline and after 16 weeks of treatment with exenatide, and centrifuged at 4000 rpm for 10 min for serum collection. The laboratory tests performed for serum chemistry analysis included HbA1c, lipid profiles, hepatic parameters, and renal function. Biochemical analysis of endothelial injury marker levels in serum was performed by using enzyme-linked immunosorbent assay (ELISA) for soluble endothelial cell protein C receptor (sEPCR; Dogesce Biotechnology, Beijing, China) and von Willebrand factor (vWF; Abcam, Cambridge, MA, USA). To avoid the possible bias induced by different exenatide origins on the levels of serum endothelial injury markers, only 93 patients who were treated with the branded exenatide injection were selected to evaluate the effect of exenatide on endothelial injury markers. The clinical characteristics of patients with and without the evaluation of endothelial injury markers were almost identical (Supplementary Table 1).

Glycemic Metrics

Fingerstick blood glucose levels that were monitored seven times a day (before three meals, 2 h after three meals, and at bedtime) were obtained from all patients at baseline and at the interval of every 2 weeks after treatment with exenatide. The values of derived TIR, time above range (TAR), and time below range (TBR) were calculated as the percentage of blood glucose values within the target range of 3.9–10.0 mmol/L, above 10 mmol/L and below 3.9 mmol/L, respectively. Moreover, the parameters of glycemic variability, including standard deviation of blood glucose (SDBG), largest amplitude of glycemic excursions (LAGE), and postprandial glucose excursions (PPGE), were calculated as reported previously [14-16].

Statistical Analyses

The Shapiro–Wilk test was applied to test for the normality of continuous variables. Data are presented as mean ± standard deviation (SD) or median (interquartile range), as appropriate. For comparison of two groups, Student’s t test and Wilcoxon signed-rank test were used for the normally distributed variables and non-normally distributed variables, respectively. Categorical variables were reported as number (%) and Pearson’s chi-squared test was used to compare differences between groups. Univariate and multivariate logistic regression analyses were performed to evaluate the baseline parameters predicting derived TIR value after the exenatide treatment. P < 0.05 was considered as statistical significance.

Results

Clinical Characteristics of Participants at Baseline and After Treatment with Exenatide for 16 Weeks

A total of 200 patients with T2DM receiving the exenatide treatment were included in this study. Compared with baseline, a significant reduction in body weight (77.7 ± 14.8 kg vs. 79.7 ± 15.0 kg, P < 0.001), body mass index (27.7 ± 4.04 kg/m2 vs. 28.4 ± 4.14 kg/m2, P < 0.001), diastolic blood pressure (76.9 ± 7.38 mmHg vs. 78.9 ± 8.27 mmHg, P = 0.001), fasting blood glucose (8.09 ± 2.20 mmol/L vs. 9.39 ± 1.79 mmol/L, P < 0.001), postprandial 2 h blood glucose (13.3 ± 4.05 mmol/L vs. 16.2 ± 3.48 mmol/L, P < 0.001), and HbA1c (7.07 ± 1.16 % vs. 8.19 ± 0.90 %, P < 0.001) level was observed after treatment with exenatide for 16 weeks in patients with T2DM whose blood glucose levels were inadequately controlled with monotherapy or combination therapy of metformin and insulin secretagogues (Table 1). There were no significant differences between baseline and post-treatment in terms of lipid profiles, hepatic parameters, and renal function (all P > 0.05).

Table 1

Clinical characteristics before and after treatment with exenatide for 16 weeks in patients with T2DM

Parameters	Baseline	After treatment	t/Z value	P value
Age, years	49.6 ± 9.83
Sex–male	119 (59.5)
Duration of diabetes, years	6.15 ± 4.66
Background therapies
Metformin	89 (44.5)
Insulin secretagogues	18 (9.0)
Both	93 (46.5)
Category of exenatide–branded	98 (49.0)
Body weight, kg	79.7 ± 15.0	77.7 ± 14.8	8.521	< 0.001
Body mass index, kg/m2	28.4 ± 4.14	27.7 ± 4.04	9.022	< 0.001
Systolic blood pressure, mmHg	126.6 ± 12.8	125.7 ± 11.7	1.047	0.296
Diastolic blood pressure, mmHg	78.9 ± 8.27	76.9 ± 7.38	3.341	0.001
Fasting blood glucose, mmol/L	9.39 ± 1.79	8.09 ± 2.20	8.018	< 0.001
Postprandial 2 h blood glucose, mmol/L	16.2 ± 3.48	13.3 ± 4.05	9.101	< 0.001
Glycated hemoglobin A1c, %	8.19 ± 0.90	7.07 ± 1.16	12.934	< 0.001
Glycated hemoglobin A1c, mmol/mol	66.0 ± 9.81	53.8 ± 12.7	12.934	< 0.001
Total cholesterol, mmol/L	4.84 ± 0.92	4.79 ± 0.92	0.973	0.350
Low-density lipoprotein cholesterol, mmol/L	2.93 ± 0.83	2.92 ± 0.82	0.077	0.939
High-density lipoprotein cholesterol, mmol/L	1.25 ± 0.38	1.21 ± 0.32	1.640	0.103
Triglycerides, mmol/L	1.65 (1.21, 2.63)	1.66 (1.20, 2.51)	− 0.999	0.319
Uric acid, μmol/L	318.4 ± 87.8	325.0 ± 86.0	− 1.244	0.215
Alanine aminotransferase, U/L	24.2 (16.4, 39.8)	23.5 (16.2, 39.0)	1.706	0.090
Aspartate aminotransferase, U/L	25.0 (18.0, 32.0)	23.0 (18.0, 31.0)	0.679	0.498
Alkaline phosphatase, U/L	68.8 (57.2, 83.8)	67.0 (56.3, 84.0)	1.586	0.114
Creatinine, μmol/L	64.2 ± 14.7	65.1 ± 14.9	− 1.200	0.231
Blood urea nitrogen, mmol/L	4.91 ± 1.30	4.85 ± 1.33	0.754	0.451
Derived TIR, %	42.9 (14.9, 71.4)	85.7 (57.1, 100.0)	− 7.912	< 0.001
SDBG, mmol/L	2.36 (1.74, 2.98)	1.64 (1.11, 2.39)	− 6.553	< 0.001
LAGE, mmol/L	6.40 (4.80, 8.00)	4.50 (3.00, 6.75)	− 6.339	< 0.001
PPGE, mmol/L	3.27 (2.01, 4.43)	2.20 (1.38, 3.22)	− 6.094	< 0.001

Data are presented as number (%), mean ± SD or median (interquartile range), as appropriate

TIR time in range, SDBG standard deviation of blood glucose, LAGE largest amplitude of glycemic excursions, PPGE postprandial glucose excursions

Effects of Exenatide Treatment on Derived TIR and Glycemic Variability

After treatment with exenatide for 16 weeks, derived TIR value increased from baseline in a time-dependent manner, with a significant difference starting from the second week of treatment (Fig. 1). At the end of the 16-week treatment, the derived TIR value significantly increased in patients with T2DM when compared with baseline [85.7 (57.1, 100.0) % vs. 42.9 (14.9, 71.4) %, P < 0.001] (Table 1 and Fig. 1). Compared with baseline, the parameters of glycemic variability, such as SDBG [1.64 (1.11, 2.39) mmol/L vs. 2.36 (1.74, 2.98) mmol/L, P < 0.001], LAGE [4.50 (3.00, 6.75) mmol/L vs. 6.40 (4.80, 8.00) mmol/L, P < 0.001], and PPGE [2.20 (1.38, 3.22) mmol/L vs. 3.27 (2.01, 4.43) mmol/L, P < 0.001] significantly decreased after the treatment (Table 1 and Fig. 2). In addition, the similar effects of exenatide on the derived TIR and the parameters of glycemic variability were observed in subgroups of patients with and without the evaluation of endothelial injury markers (Supplementary Figs. 2 and 3).

Fig. 1

Changes in derived TIR value from baseline during the exenatide treatment. Data are presented as median (interquartile range). *P < 0.05 vs. baseline. TIR time in range

Fig. 2

Changes in the parameters of glycemic variability from baseline during the exenatide treatment. Data are presented as median (interquartile range). *P < 0.05 vs. baseline. SDBG standard deviation of blood glucose, LAGE largest amplitude of glycemic excursions, PPGE postprandial glucose excursions

Baseline Variables Predicting Derived TIR Value Induced by Exenatide Treatment

Results of univariate logistic regression analysis of baseline variables in relation to derived TIR value are listed in Table 2. The results showed that younger age, shorter duration of diabetes, lower blood glucose concentration at postprandial 2 h or fasting state, lower HbA1c level, smaller alkaline phosphatase value, and higher high-density lipoprotein cholesterol level at baseline were associated with higher derived TIR value after treatment with exenatide. To investigate the independent effects, the factors with significant differences in univariate logistic analysis were further analyzed by using stepwise multivariate logistic regression. As shown in Fig. 3, HbA1c level (OR 0.534; 95% CI 0.359, 0.793) and duration of diabetes (OR 0.885; 95% CI 0.807, 0.971) at baseline were associated with higher derived TIR value after the treatment.

Table 2

Association of baseline parameters with derived TIR after the exenatide treatment

	Derived TIR
	OR	95% CI	P value
Age	0.959	0.931, 0.989	0.008
Sex	0.908	0.502, 1.642	0.749
Duration of diabetes	0.863	0.795, 0.936	< 0.001
Body weight	1.009	0.989, 1.028	0.389
Body mass index	1.006	0.937, 1.079	0.837
Systolic blood pressure	0.987	0.964, 1.010	0.275
Diastolic blood pressure	0.993	0.959, 1.029	0.699
Fasting blood glucose	0.460	0.351, 0.603	< 0.001
Postprandial 2 h blood glucose	0.664	0.583, 0.757	< 0.001
Glycated hemoglobin A1c	0.566	0.394, 0.813	0.002
Total cholesterol	0.845	0.621, 1.149	0.283
Low-density lipoprotein cholesterol	0.841	0.596, 1.187	0.326
High-density lipoprotein cholesterol	2.959	1.343, 6.521	0.007
Triglycerides	0.899	0.688, 1.175	0.437
Uric acid	1.002	0.999, 1.006	0.165
Alanine aminotransferase	0.993	0.980, 1.007	0.321
Aspartate aminotransferase	0.992	0.967, 1.017	0.513
Alkaline phosphatase	0.981	0.965, 0.996	0.015
Creatinine	0.977	0.977, 1.017	0.777
Blood urea nitrogen	0.837	0.663, 1.058	0.136

Fig. 3

Forest plot of the multivariate logistic regression for the baseline factors associated with derived TIR value. LDL-C low-density lipoprotein cholesterol, HbA1c glycated hemoglobin A1c

Attainment of TIR Target is Associated with Improvement of Endothelial Injury in Patients Treated with Exenatide

According to the values of derived TIR at 16 weeks, the patients were divided into two groups (TIR > 70% and TIR ≤ 70%). Compared with baseline, a significant reduction in body weight (78.1 ± 14.3 kg vs. 80.6 ± 14.8 kg, P < 0.001), fasting blood glucose (7.30 ± 1.52 mmol/L vs. 9.12 ± 1.73 mmol/L, P < 0.001), alkaline phosphatase [65.0 (56.0, 79.0) U/L vs. 67.0 (57.0, 80.6) U/L, P = 0.005], SDBG [1.30 (0.98, 1.87) mmol/L vs. 2.21 (1.65, 2.87) mmol/L, P < 0.001], LAGE [3.80 (2.50, 5.20) mmol/L vs. 6.00 (4.30, 7.90) mmol/L, P < 0.001], and PPGE [1.87 (1.20, 2.60) mmol/L vs. 3.07 (1.73, 4.10) mmol/L, P < 0.001] was observed in patients with TIR > 70% but not in those with TIR ≤ 70% after treatment with exenatide (Table 3). Importantly, serum sEPCR level was significantly decreased from baseline in patients with TIR > 70% [74.5 (32.8, 122.5) ng/mL vs. 96.9 (48.5, 150.9) ng/mL, P = 0.006] but not in those with TIR ≤ 70% after the treatment. Serum vWF level was remarkably decreased from baseline in patients with TIR > 70% [907.4 (674.3, 1335.1) mIU/mL vs. 1166.2 (848.1, 1335.5) mIU/mL, P = 0.001], while this effect was modest in those with TIR ≤ 70% [1168.2 (802.2, 1455.3) mIU/mL vs. 1245.8 (1022.0, 1812.6) mIU/mL, P = 0.009] after the exenatide treatment (Fig. 4 and Supplementary Table 2).

Table 3

Clinical characteristics before and after treatment with exenatide for 16 weeks in patients with TIR > 70% and TIR ≤ 70%

Parameters	TIR > 70% (n = 136)		t/Z value	P value	TIR ≤ 70% (n = 64)		t/Z value	P value
	Baseline	After treatment			Baseline	After treatment
Age, years	48.1 ± 10.3				51.5 ± 8.61
Sex–male	80 (58.8)				39 (56.3)
Duration of diabetes, years	5.52 ± 4.35				7.46 ± 5.04
Background therapies
Metformin	64 (47.1)				25 (39.0)
Insulin secretagogues	9 (6.6)				9 (14.1)
Both	63 (46.3)				30 (46.9)
Body weight, kg	80.6 ± 14.8	78.1 ± 14.3	10.888	< 0.001	77.9 ± 15.5	76.8 ± 15.6	1.925	0.059
Body mass index, kg/m2	28.7 ± 4.14	27.8 ± 4.00	10.989	< 0.001	27.8 ± 4.11	27.4 ± 4.13	2.313	0.024
Systolic blood pressure, mmHg	126.7 ± 13.1	126.2 ± 12.4	0.466	0.642	126.5 ± 12.4	124.6 ± 10.1	1.195	0.237
Diastolic blood pressure, mmHg	78.6 ± 8.24	77.0 ± 7.27	2.196	0.030	79.6 ± 8.36	76.8 ± 7.68	2.710	0.009
Fasting blood glucose, mmol/L	9.12 ± 1.73	7.30 ± 1.52	10.784	< 0.001	9.95 ± 1.80	9.79 ± 2.44	0.524	0.602
Postprandial 2 h blood glucose, mmol/L	15.8 ± 3.49	12.4 ± 3.51	9.900	< 0.001	16.9 ± 3.37	15.4 ± 4.37	2.487	0.016
Glycated hemoglobin A1c, %	8.06 ± 0.87	6.58 ± 0.79	15.833	< 0.001	8.48 ± 0.89	8.12 ± 1.13	2.492	0.015
Glycated hemoglobin A1c, mmol/mol	64.6 ± 9.52	48.5 ± 8.61	15.833	< 0.001	69.1 ± 9.77	65.2 ± 12.4	2.492	0.015
Total cholesterol, mmol/L	4.77 ± 0.90	4.77 ± 0.93	0.091	0.928	5.01 ± 0.96	4.85 ± 0.92	1.437	0.156
Low-density lipoprotein cholesterol, mmol/L	2.90 ± 0.83	2.93 ± 0.83	− 0.479	0.633	2.99 ± 0.83	2.91 ± 0.79	0.672	0.504
High-density lipoprotein cholesterol, mmol/L	1.22 ± 0.34	1.21 ± 0.30	0.944	0.347	1.29 ± 0.46	1.23 ± 0.37	1.352	0.181
Triglycerides, mmol/L	1.65 (1.22, 2.54)	1.58 (1.17, 2.38)	− 0.026	0.979	1.67 (1.16, 2.76)	1.90 (1.32, 2.69)	− 1.344	0.179
Uric acid, μmol/L	322.5 ± 83.8	332.1 ± 84.5	− 1.415	0.160	309.8 ± 95.7	309.9 ± 87.8	− 0.018	0.986
Alanine aminotransferase, U/L	23.4 (16.4, 37.8)	22.0 (16.2, 33.0)	− 1.886	0.059	25.2 (16.4, 44.5)	28.1 (17.1, 47.0)	− 0.664	0.507
Aspartate aminotransferase, U/L	23.0 (18.0, 30.4)	22.9 (18.0, 28.5)	− 1.536	0.125	28.5 (19.0, 34.7)	27.0 (18.2, 38.1)	− 0.379	0.704
Alkaline phosphatase, U/L	67.0 (57.0, 80.6)	65.0 (56.0, 79.0)	− 2.803	0.005	72.5 (58.8, 87.2)	74.5 (60.0, 87.7)	− 0.883	0.377
Creatinine, μmol/L	64.4 ± 14.5	65.7 ± 14.7	− 1.538	0.126	63.9 ± 15.1	63.8 ± 15.5	0.037	0.971
Blood urea nitrogen, mmol/L	4.81 ± 1.18	4.80 ± 1.36	0.101	0.919	5.11 ± 1.52	4.94 ± 1.25	1.087	0.281
SDBG, mmol/L	2.21 (1.65, 2.87)	1.30 (0.98, 1.87)	− 7.460	< 0.001	2.62 (2.05, 3.59)	2.50 (1.98, 2.95)	− 1.004	0.315
LAGE, mmol/L	6.00 (4.30, 7.90)	3.80 (2.50, 5.20)	− 6.863	< 0.001	7.10 (5.45, 9.85)	6.55 (5.10, 7.95)	− 1.278	0.201
PPGE, mmol/L	3.07 (1.73, 4.10)	1.87 (1.20, 2.60)	− 6.633	< 0.001	3.80 (2.85, 4.73)	3.43 (2.69, 4.28)	− 1.253	0.210

Data are presented as number (%), mean ± SD or median (interquartile range), as appropriate

TIR time in range, SDBG standard deviation of blood glucose, LAGE largest amplitude of glycemic excursions, PPGE postprandial glucose excursions

Fig. 4

Changes of endothelial injury markers in patients with derived TIR > 70% and ≤ 70% after the exenatide treatment. Data are presented as median (interquartile range) and each dot represents an individual value. *P < 0.05 vs. baseline. sEPCR soluble endothelial cell protein C receptor, vWF von Willebrand factor

Discussion

In the present study, we demonstrated that treatment with exenatide for 16 weeks improved glycemic control and lowered body weight and diastolic blood pressure in patients with T2DM whose HbA1c levels were inadequately controlled by either monotherapy or combined therapy with metformin and insulin secretagogues. Moreover, the exenatide treatment resulted in an increased derived TIR value and a decreased glycemic variability as indicated by SDBG, LAGE, and PPGE. In addition, lower baseline HbA1c level and shorter duration of diabetes were identified as independent predictors for higher derived TIR value after treatment with exenatide. Notably, the amelioration of endothelial injury, characterized by the decreased levels of sEPCR and vWF, was more pronounced in patients with TIR > 70% than in those with TIR ≤ 70% after the exenatide treatment.

Several clinical studies have shown that treatment with exenatide improves glycemic control and reduces body weight in patients with T2DM [17, 18]. Similarly, this study indicated that treatment with exenatide for 16 weeks in patients with T2DM not only significantly lowered fasting blood glucose, postprandial 2 h blood glucose, and HbA1c levels but also remarkably reduced body weight and body mass index.

With growing evidence of correlation between TIR and diabetic vascular complications, TIR has been recommended as an important parameter to evaluate the favorable glycemic control by recent international guidelines and consensus [5, 19]. Glucose-lowering agents, such as teneligliptin (a dipeptidyl peptidase 4 inhibitor), dapagliflozin (a sodium-glucose cotransporter 2 inhibitor), and acarbose (an α-glucosidase inhibitor), have been demonstrated to increase TIR value in patients with T2DM [20-22]. However, whether exenatide, the first approved GLP-1RA, can increase TIR value is still unknown. In the present study, we found that treatment with exenatide for 16 weeks in patients with T2DM significantly increased derived TIR value. To the best of our knowledge, this is the first report to evaluate the effect of exenatide on TIR value in patients with diabetes.

Our study also showed that the parameters of glycemic variability, including SDBG, LAGE, and PPGE, were significantly decreased after treatment with exenatide, indicating that exenatide was potent in reducing blood glucose fluctuations in patients with T2DM. These results are in accordance with findings from a previous study, in which treatment with exenatide for 16 weeks markedly decreased SDBG, LAGE, and mean amplitude of glycemic excursions. However, a small sample size of participants (only 19 patients with T2DM) was included in that study [23]. This study included 200 patients with T2DM and made the observations that exenatide decreased glycemic variability more concrete.

Identification of the baseline parameters to predict which patients derive the most benefit from treatment with exenatide will help precision diabetes management [24]. We demonstrated that shorter duration of diabetes and lower HbA1c level at baseline were independently associated with higher derived TIR value, which indicated that patients with T2DM whose duration of diabetes was shorter and baseline HbA1c level was lower would benefit more from the exenatide treatment in terms of TIR control. This finding might be partly explained by the fact that shorter duration of diabetes and lower HbA1c level reflected better pancreatic β cell function, and exenatide was more prone to increase TIR in this subgroup of patients with T2DM [25, 26].

The results of cardiovascular outcome trials and meta-analysis show that GLP-1RAs can not only reduce the risk of major adverse cardiovascular events but also prevent the development of diabetic microvascular complications [11, 12], indicating that GLP-1RAs have preventive and therapeutic effects on diabetic vascular complications. It has been demonstrated that endothelial dysfunction is a critical mechanism of diabetic vascular complications, and amelioration of endothelial injury can help to prevent the progression of these complications [27]. In our previous studies, treatment with exenatide in patients with T2DM significantly decreased the levels of endothelial injury markers like soluble vascular cell adhesion molecule 1, intercellular cell adhesion molecule 1, soluble thrombomodulin, sEPCR, and vWF [28, 29]. However, which subgroup of people with T2DM will benefit more from the amelioration of endothelial injury remains unclear. In the present study, we found that the levels of the endothelial injury markers sEPCR and vWF were more significantly decreased in patients who achieved the goal of TIR > 70% than in those with TIR ≤ 70% after the exenatide treatment. These results indicated that elevation of TIR value had an important role in the improvement of endothelial dysfunction in patients with T2DM treated with exenatide.

There are some limitations in our study. First, seven-point fingerstick blood glucose monitoring is somewhat limited in assessing TIR and glycemic variability for individuals with diabetes. However, TIR derived from the seven-point blood glucose monitoring has been reported to have a strong association with the risk of the development and/or progression of diabetic retinopathy and diabetic kidney disease in several other studies [6, 30]. Second, even though 200 patients with T2DM were included in the present study, larger number of patients with T2DM would be required to confirm our findings. Third, this is a post hoc analysis with only single-arm intervention. Randomized clinical trials having a control group without treatment with exenatide can provide stronger evidence.

Conclusions

Our study demonstrates that treatment with exenatide for 16 weeks improves glycemic control and lowers body weight in patients with T2DM. Moreover, treatment with exenatide results in improved TIR control and decreased glycemic variability. In addition, lower baseline HbA1c level and shorter duration of diabetes are identified as independent predictors for better TIR control in the patients treated with exenatide. Importantly, the amelioration of endothelial injury is more pronounced in patients with TIR > 70% than in those with TIR ≤ 70% after treatment with exenatide.

Below is the link to the electronic supplementary material.

Supplementary file1 (PDF 319 KB)

Why carry out this study?

Intensive glycemic control can decrease the incidence of diabetic vascular complications and reduce all-cause mortality.

Whether time in range (TIR) is associated with vascular endothelial dysfunction in patients with type 2 diabetes treated with exenatide remains to be clarified.

This study aimed to investigate the association between TIR and endothelial injury markers in patients with type 2 diabetes treated with exenatide.

What was learned from the study?

Treatment with exenatide increases TIR and decreases glycemic variability in patients with T2DM.

The amelioration of endothelial injury is more pronounced in patients with TIR > 70% after the treatment with exenatide.