Evaluation of a Patient With Non-Myoinvasive Uterine Serous Carcinoma Confined to a Polyp and Positive Peritoneal Washings With Somatic ARHGAP35 and KRAS Mutations.

Currently, the application of peritoneal washings as a diagnostic tool for endometrial cancer staging is not well defined. The case described aims to highlight the current ambiguity surrounding the use of peritoneal washings in clinical practice.  A 69-year-old G3P3003 presented to her gynecologist with complaints of new-onset heavy vaginal bleeding. The patient sought an endometrial biopsy, which suggested serous endometrial intraepithelial carcinoma (EIC) focally suspicious for invasive carcinoma, with the involvement of polyps. Based on these results, a robotic-assisted total laparoscopic hysterectomy, bilateral salpingo-oophorectomy, bilateral sentinel lymph node dissection, and omentectomy were performed. Results from her final pathology exhibited a stage IA uterine serous carcinoma (USC) involving a polyp (4.2 cm in greatest dimension) with no myometrial or lymphovascular invasion, but washings were positive for adenocarcinoma. Based on her family history of malignancy, the patient underwent germline panel testing. The patient's somatic tumor testing demonstrated proficient DNA mismatch repair status, microsatellite stability, low tumor mutational burden (4 mut/Mb), low loss of heterozygosity (9%), amplification of the ERBB2 (HER2/neu) gene by both immunohistochemistry (3+, 20% positive) and fluorescence in-situ hybridization. Her tumor also had weakly positive estrogen receptor expression (1+, 10% positive); furthermore, some pathogenic variants in KRAS (c.37G>T), PIK3CA (c.263G>A), and TP53 (c.743G>A) were identified. Given the incongruent findings found with the positive peritoneal washing and negative lymph node involvement in addition to molecular testing, management for this patient was unclear. Ultimately, this case highlights a number of advances within the field of gynecological oncology but also emphasizes the persistent ambiguity and incongruency in the management of patients with early-stage high-risk histologies. Moving forward it will become increasingly important to be able to develop a more standardized process to assess how these diagnostic tools should inform prognosis and treatment plans.

Introduction

Endometrial cancer represents the most common gynecologic cancer in the United States with around 66,570 new cases estimated to be diagnosed in 2021 [1]. Only about 10% of endometrial cancers are uterine serous carcinomas (USC), but they make up around 40% of deaths [2]. USC is high-grade by definition and more aggressive than most endometrioid endometrial adenocarcinomas [2]. USC has a propensity to invade local lymphatics and other intra-peritoneal structures, sometimes even in the absence of identifiable myometrial invasion, which is why many women are diagnosed with advanced-stage disease and have relatively poorer prognoses compared to women with endometrioid adenocarcinoma [3].

USC, like all endometrial cancers, is staged using the FIGO (Fédération Internationale de Gynécologie et d’Obstétrique) staging system which was revised in 2009. Prior to this time, peritoneal washings were considered an important component of endometrial cancer staging, in order to rule out the presence of malignant cells in the peritoneal cavity. Numerous studies questioned the significance of peritoneal cytology and upon investigation found little to no evidence that it was an independent prognostic factor [4-6]. These findings led to the 2009 revision of the FIGO staging system, removing peritoneal washings from the staging guidelines [7]. Despite this revision, providers have continued to use peritoneal washings as part of their diagnostic evaluation, and more recent studies have found positive results correlating with poorer prognosis [8-12]. While peritoneal cytology does not currently affect staging, FIGO, the American Joint Committee on Cancer, and the National Comprehensive Cancer Network continue to recommend obtaining washings because positive cytology may add to the effect of other poor prognostic factors and impact treatment decision-making [13].

We also have a new molecular understanding of USC based in part on work published by The Cancer Genome Atlas Network (TCGA) [14]. Where previously endometrial cancer was classified into two subgroups defined by clinical and pathologic features, comprehensive profiling by TCGA classified four separate endometrial cancer genomic signatures that could augment or replace this dualistic model and individualize treatment decision-making [15,16]. In the TCGA study, serous tumors were characterized by frequent mutations in TP53, extensive copy number alterations, and few methylation changes [14]. KRAS mutations were not common in this group (only 1%) of all USC cases whereas they were much more common in endometrioid cancer.

The following report demonstrates an interesting case of a woman with USC who had positive peritoneal washings despite non-invasive disease and was found on comprehensive tumor profiling to have a number of somatic tumor mutations which may have contributed to the aggressiveness of her disease.

Case presentation

A 69-year-old G3P3003 presented to her gynecologist with complaints of new-onset heavy vaginal bleeding. She denied abdominal pain, dysuria, urinary frequency or urgency, weight loss, fevers, or recent injury or trauma. This was her first episode of bleeding since she became menopausal in her mid-50s. Her past medical history was significant for three spontaneous vaginal deliveries. She also had HER2/neu positive ductal carcinoma for which she had a lumpectomy and completed 10 years of anastrozole suppression and was in disease remission. Notably, the patient had a strong family history of cancer: the patient’s mother had breast and colon cancer, maternal grandmother had both breast and stomach cancer, maternal grandfather had pancreatic cancer, and a cousin had prostate cancer. Other than obesity (with a BMI of 36), she reported no other relevant medical history.

Her gynecologist performed an endometrial biopsy demonstrating at least serous endometrial intraepithelial carcinoma (EIC) focally suspicious for invasive carcinoma, with involvement of polyps. She had a normal CA125 (17.2 U/mL) and a CT scan of her chest abdomen and pelvis which showed a low-attenuating lesion in the central portion of the uterus but no lymphadenopathy or other evidence of metastatic disease. Based on these results, she was referred to a gynecologic oncologist who performed a robotic-assisted total laparoscopic hysterectomy, bilateral salpingo-oophorectomy, bilateral sentinel lymph node dissection, and omentectomy.

Her final pathology results showed a stage IA USC involving a polyp (4.2 cm in greatest dimension) with no myometrial invasion, no lymphovascular invasion, and three negative sentinel lymph nodes but washings positive for adenocarcinoma (Figures 1-3). Her immunohistochemical tests demonstrated intact expression of DNA mismatch repair proteins (MLH1, MSH2, MSH6, PMS2).

Figure 1

Hematoxylin and eosin stain demonstrating endometrial intraepithelial carcinoma involving a polyp. Original magnification x 100.

Figure 3

Cytology from pelvic washings demonstrating positivity for adenocarcinoma. Original magnification x 400.

Based on her family history of malignancy, the patient underwent germline panel testing of 47 genes frequently associated with hereditary cancers via the Invitae Common Hereditary Cancer Panel (https://www.invitae.com/). Other than a variant of uncertain significance in BARD1 (c.1409A>G; p.ASN470Ser), she had no deleterious germline mutations identified. Additionally, an archival tumor block was sent for Caris Molecular Intelligence testing. This tumor profiling strategy combines whole-exome sequencing, whole transcriptomic sequencing, and immunohistochemistry to examine cancer-type relevant biomarkers. Details regarding this assay can be found at Caris Life Sciences: Comprehensive Molecular Profiling (https://www.carismolecularintelligence.com/comprehensivetumorprofiling.)

The patient’s somatic tumor testing was notable for proficient DNA mismatch repair status, microsatellite stability, low tumor mutational burden (4 mut/Mb), low loss of heterozygosity (9%), amplification of the ERBB2 (Her2/Neu) gene by both immunohistochemistry (3+, 20% positive) and fluorescence in-situ hybridization. Her tumor also had weakly positive estrogen receptor expression (1+, 10% positive) but negative progesterone receptor staining (0%). There were notable somatic pathogenic variants in KRAS (c.37G>T), PIK3CA (c.263G>A), and TP53 (c.743G>A).

Given the results from the molecular profiling along with the incongruent findings found with the positive peritoneal washing and negative lymph node involvement, the steps to manage this patient were not well defined. Ultimately, the patient was presented at the tumor board where it was decided that she would proceed with six cycles of carboplatin and paclitaxel followed by cuff brachytherapy. This case, however, created an opportunity to discuss the current space for peritoneal washings in informing treatment regimens, and the way molecular profiling can help direct the management of patients that present with early-stage USC.

Discussion

Our understanding of the prognostic value of peritoneal cytology has been evolving since the 2009 FIGO staging modifications [11]. An estimated 11% of women who undergo staging surgery for endometrial cancer will have positive peritoneal cytology, most commonly in the presence of extra-uterine disease. The prognostic significance of isolated peritoneal cytology in the absence of other risk factors is controversial and therefore it is not often used as a high-risk criterion in the formulation of adjuvant treatment planning.

Several recent studies have found peritoneal cytology to be an important prognostic for survival in women with early-stage endometrial cancer [9,10]. One study conducted by Matsuo et al. found that in a population of 1668 women with early-stage endometrioid cancer, the presence of abnormal peritoneal cytology (11% of their cohort) was independently associated with decreased disease-free survival (HR 3.07, P<0.001) and cause-specific survival (HR 3.42, P = 0.008) [9]. Another recent study out of Michigan examined a cohort of 148 women with USC and found that 22% of the cohort had positive washings. The presence of malignant cytology in this study was significantly associated with recurrence and overall survival (HR 2.09, 95% CI [1.19-3.68]) [17]. A large Surveillance, Epidemiology, and End Results database study of over 14,000 patients with uterine cancer identified that five-year disease-specific survival for women with negative cytology was significantly better than those with positive cytology (95.1% vs 80.8% in endometrioid adenocarcinoma and 78.0% vs 50.4% in clear cell or serous carcinoma) [18]. Because of this, some experts have proposed a new staging schema with stage-specific incorporation of malignant peritoneal cytology [19].

These findings become especially relevant when evaluating a patient with peritoneal washing results that do not align with the degree of myometrial invasion, presence of lymphovascular invasion, or of nodal involvement. In situations like this case, if abnormal peritoneal cytology is a prognostic factor for decreased survival, as has been suggested in these studies, it may be necessary to approach treatment more aggressively [8].

We know that women with the completely staged disease and no residual tumor in their hysterectomy specimen after dilation and curettage or hysteroscopic resection do not often experience recurrence regardless of adjuvant therapy. However, multiple studies have demonstrated that women with early-stage USC experience improved disease-free survival and overall survival with platinum-based chemotherapy [2,20]. It remains unclear if women with early-stage high-risk histologies with positive pelvic washings would benefit from aggressive adjuvant therapy in the absence of other risk factors for recurrence. Mysona et al. published a case series of 1751 patients with stage IA USC in which 7% had malignant cytology [21]. The presence of positive peritoneal cytology was associated in multivariate analysis with an increased hazard of death (HR 2.62, 95% CI [1.79-3.82]). This group also produced a nomogram based on Cox regression analysis to help predict which women with stage IA USC would benefit from adjuvant chemotherapy. However, in their backward recursive elimination, peritoneal cytology was left out of their nomogram. Based on her age and tumor size, this Cox-based nomogram would have classified her case as “moderate risk” with a slightly greater than 70% probability of surviving at five years. In their group of moderate-risk women, chemotherapy improved five-year survival by 9%. They also published a clinical calculator based on a random survival forest approach which would have given our patient a score of 8.45, which suggests she would not have benefited from chemotherapy. These examples highlight the difficulty of risk-stratification of women with early-stage high-risk histologies and determining for whom adjuvant treatment is most appropriate.

In addition to reassessing the role of peritoneal washings in informing this patient’s treatment, it is important to take into consideration the results of her molecular testing. Recent advancements in molecular testing in this patient population have created opportunities for novel treatment modalities [22]. One area of current exploration is the role of adding trastuzumab to adjuvant chemotherapy in HER2+ serous cancers. Recent studies have revealed HER2/neu amplification to be present in 18-42% of USC patients [23]. These results suggest that HER2 therapies based on monoclonal antibodies or tyrosine kinase inhibitors could be an effective new approach to treating patients with USC [24]. Trastuzumab is a monoclonal antibody that specifically targets HER2/neu [25]. Early studies investigating trastuzumab combined with carboplatin and paclitaxel chemotherapy in advanced and recurrent USC patients overexpressing HER2/neu have shown promising results. In a landmark phase II trial of women with stage III-IV or recurrent HER2 over-expressing uterine serous carcinomas, the addition of trastuzumab to carboplatin and paclitaxel as adjuvant treatment improved overall survival by over five months (HR 0.58; 95% [CI 0.34-0.99]) [26]. More recent studies looking specifically at the benefit of this approach in early-stage HER2/neu positive patients found patients who received trastuzumab also showed improved progression-free survival. The greatest benefit, however, was still identified in patients with stage III-IV disease [23]. While only a handful of studies have been conducted in early-stage patients, they are starting to show promising results [23,27]. It remains unclear if the cost of this medication would be justifiable (or even beneficial) in women with early-stage, HER2 overexpressing disease.

This patient also had TP53 and PIK3CA mutations with a low mutation frequency and microsatellite stable. These results are not unusual for this patient population but do have implications on prognosis and treatment [28]. In one study it was found that individuals with both TP53 and PIK3CA mutations had worse survival prognoses than those with only TP53 mutation [29]. PIK3CA mutations, specifically, have also been implicated in the efficacy of HER2-targeted therapy. Despite the promise of HER2 targeted therapy, PIK3CA mutations may serve as a mechanism by which resistance to trastuzumab is acquired. Black et al found a statistically significant increase in the prevalence of acquired trastuzumab resistance in the presence of PIK3CA mutations using mouse models [30]. Nevertheless, both these mutations, TP53 and PIK3CA, offer promising alternative therapeutic targets for USC. Currently, clinical trials are being conducted to assess the efficacy of both WEE1, a key cell cycle regular, and PI3K/AKT/mTOR inhibitors. Both studies have already begun to report promising results when using these targeted therapies [25,31]. Overall, these studies highlight the significance of these molecular markers as both prognostic factors and therapeutic targets, especially in early-stage HER2+ USC patients.

This patient’s tumor also had several other mutations worth noting including a truncation in ARHGAP35 and a very rarely occurring KRAS variant. Mutations in ARHGAP35 were found in 18% of the uterine serous cancers in TCGA making it one of the most frequently mutated genes in this cancer type [32,33]. Whereas ARHGAP35 mutations often occur simultaneously with TP53 mutations in USC, molecular data from carcinosarcomas demonstrate these mutations occur more commonly in tumors without TP53 mutations [34]. The role of ARHGAP35 mutations in carcinogenesis is still not completely understood [35]. This gene is associated with the regulation of RhoA, a transforming protein associated with the regulation of the cytoskeleton [36]. Despite the emergence of ARHGAP35 mutations as a new major cancer gene, few studies have investigated its implication for the prognosis and treatment of USC. The ARHGAP35 mutation in this study was a truncation (p.R617) suggesting loss of ARHGAP35 normal functions.

A KRAS G13C mutation was also identified in this patient’s tumor molecular data. While KRAS mutation is common in endometrioid endometrial cancers it is rare in uterine serous tumors occurring in less than 3% of cases. This particular mutation was not seen in any other serous case. Despite the rarity of this mutation, it could, however, make this patient eligible for pan-RAS inhibitors which target KRAS mutations. No studies, however, have examined this specific mutation in this population; therefore, more investigation is required in order to understand how these results should inform prognosis and treatment. In addition to ARHGAP35 and KRAS genes, several other mutations were noted including those in BARD1 (also noted in the hereditary cancer germline analysis described above), YYAP1 (YAP), POLH, and MLH3 among others. The complete list of detected variants, amplifications, and fusion events is described in the supplemental table provided in the appendix.

Conclusions

Ultimately, this case highlights some exciting changes that have occurred in our field over the past 10 years, but also hints at persistent ambiguity and confusion in managing patients with early-stage high-risk histologies. Moving forward it will become increasingly important to be able to, firstly, integrate the results of a patient’s histopathology with their molecular testing, secondly, reconcile how these findings impact prognosis, and thirdly, develop treatment plans based on those results.

Table 1

Complete list of detected variants, amplifications, and fusion events identified in the patient’s tumor

Biomarker	Method	Analyte Descrip	Result	Variant Detail	Variant Frequency(%)
ABCB11	Seq	DNA-Tumor	Mutation Not Detected
ABL1	Seq	DNA-Tumor	Mutation Not Detected
ABL1	Seq	RNA-Tumor	Fusion Not Detected
ABL2	CNA-Seq	DNA-Tumor	Amplification Not Detected
ABL2	Seq	DNA-Tumor	Mutation Not Detected
ABRAXAS1	Seq	DNA-Tumor	Mutation Not Detected
ACD	Seq	DNA-Tumor	Mutation Not Detected
ACKR3	Seq	DNA-Tumor	Mutation Not Detected
ACSL3	CNA-Seq	DNA-Tumor	Amplification Not Detected
ACSL3	Seq	DNA-Tumor	Mutation Not Detected
ACSL6	CNA-Seq	DNA-Tumor	Amplification Not Detected
ACSL6	Seq	DNA-Tumor	Mutation Not Detected
ACVR1	Seq	DNA-Tumor	Mutation Not Detected
ACVR1B	Seq	DNA-Tumor	Indeterminate
ADGRA2	Seq	DNA-Tumor	Mutation Not Detected
AFDN	CNA-Seq	DNA-Tumor	Amplification Not Detected
AFDN	Seq	DNA-Tumor	Mutation Not Detected
AFF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
AFF1	Seq	DNA-Tumor	Mutation Not Detected
AFF3	CNA-Seq	DNA-Tumor	Amplification Not Detected
AFF3	Seq	DNA-Tumor	Mutation Not Detected
AIP	Seq	DNA-Tumor	Mutation Not Detected
AJUBA	Seq	DNA-Tumor	Mutation Not Detected
AKT1	Seq	DNA-Tumor	Mutation Not Detected
AKT2	CNA-Seq	DNA-Tumor	Amplification Not Detected
AKT2	Seq	DNA-Tumor	Mutation Not Detected
AKT3	CNA-Seq	DNA-Tumor	Amplification Not Detected
AKT3	Seq	DNA-Tumor	Mutation Not Detected
AKT3	Seq	RNA-Tumor	Fusion Not Detected
ALK	CNA-Seq	DNA-Tumor	Amplification Not Detected
ALK	Seq	DNA-Tumor	Mutation Not Detected
ALK	Seq	RNA-Tumor	Fusion Not Detected
ALOX12B	Seq	DNA-Tumor	Mutation Not Detected
AMER1	Seq	DNA-Tumor	Mutation Not Detected
ANKRD26	Seq	DNA-Tumor	Indeterminate
APC	CNA-Seq	DNA-Tumor	Amplification Not Detected
APC	Seq	DNA-Tumor	Mutation Not Detected
APLNR	Seq	DNA-Tumor	Mutation Not Detected
AR	Seq	DNA-Tumor	Mutation Not Detected
AR	Seq	RNA-Tumor	Variant Transcript Not Detected
ARAF	Seq	DNA-Tumor	Mutation Not Detected
ARFRP1	Seq	DNA-Tumor	Mutation Not Detected
ARHGAP26	Seq	RNA-Tumor	Fusion Not Detected
ARHGAP35	Seq	DNA-Tumor	Unclassified Variant	Exon 1 | p.R617	41
ARHGEF12	Seq	DNA-Tumor	Indeterminate
ARID1A	CNA-Seq	DNA-Tumor	Amplification Not Detected
ARID1A	Seq	DNA-Tumor	Mutation Not Detected
ARID1B	Seq	DNA-Tumor	Mutation Not Detected
ARID2	CNA-Seq	DNA-Tumor	Amplification Not Detected
ARID2	Seq	DNA-Tumor	Mutation Not Detected
ARID5B	Seq	DNA-Tumor	Mutation Not Detected
ARNT	CNA-Seq	DNA-Tumor	Amplification Not Detected
ARNT	Seq	DNA-Tumor	Mutation Not Detected
ASPSCR1	Seq	DNA-Tumor	Unclassified Variant	Exon 13 | p.A458T	56
ASXL1	CNA-Seq	DNA-Tumor	Amplification Not Detected
ASXL1	Seq	DNA-Tumor	Mutation Not Detected
ASXL2	Seq	DNA-Tumor	Indeterminate
ATF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
ATF1	Seq	DNA-Tumor	Mutation Not Detected
ATIC	CNA-Seq	DNA-Tumor	Amplification Not Detected
ATIC	Seq	DNA-Tumor	Mutation Not Detected
ATM	CNA-Seq	DNA-Tumor	Amplification Not Detected
ATM	Seq	DNA-Tumor	Mutation Not Detected
ATP1A1	CNA-Seq	DNA-Tumor	Amplification Not Detected
ATP1A1	Seq	DNA-Tumor	Mutation Not Detected
ATR	CNA-Seq	DNA-Tumor	Amplification Not Detected
ATR	Seq	DNA-Tumor	Mutation Not Detected
ATRX	Seq	DNA-Tumor	Mutation Not Detected
AURKA	Seq	DNA-Tumor	Indeterminate
AURKB	CNA-Seq	DNA-Tumor	Amplification Not Detected
AURKB	Seq	DNA-Tumor	Mutation Not Detected
AXIN1	Seq	DNA-Tumor	Mutation Not Detected
AXIN2	Seq	DNA-Tumor	Mutation Not Detected
AXL	Seq	DNA-Tumor	Mutation Not Detected
AXL	Seq	RNA-Tumor	Fusion Not Detected
B2M	Seq	DNA-Tumor	Mutation Not Detected
BAP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
BAP1	Seq	DNA-Tumor	Mutation Not Detected
BARD1	CNA-Seq	DNA-Tumor	Amplification Not Detected
BARD1	Seq	DNA-Tumor	Unclassified Variant	Exon 6 | p.N470S	48
BCL10	Seq	DNA-Tumor	Mutation Not Detected
BCL11A	CNA-Seq	DNA-Tumor	Amplification Not Detected
BCL11A	Seq	DNA-Tumor	Mutation Not Detected
BCL11B	Seq	DNA-Tumor	Mutation Not Detected
BCL2	Seq	DNA-Tumor	Mutation Not Detected
BCL2L1	Seq	DNA-Tumor	Mutation Not Detected
BCL2L11	CNA-Seq	DNA-Tumor	Amplification Not Detected
BCL2L11	Seq	DNA-Tumor	Mutation Not Detected
BCL2L12	Seq	DNA-Tumor	Indeterminate
BCL2L2	Seq	DNA-Tumor	Mutation Not Detected
BCL3	CNA-Seq	DNA-Tumor	Amplification Not Detected
BCL3	Seq	DNA-Tumor	Indeterminate
BCL6	CNA-Seq	DNA-Tumor	Amplification Not Detected
BCL6	Seq	DNA-Tumor	Mutation Not Detected
BCL9	CNA-Seq	DNA-Tumor	Amplification Not Detected
BCL9	Seq	DNA-Tumor	Mutation Not Detected
BCOR	Seq	DNA-Tumor	Mutation Not Detected
BCORL1	Seq	DNA-Tumor	Mutation Not Detected
BCR	Seq	RNA-Tumor	Fusion Not Detected
BIRC3	Seq	DNA-Tumor	Indeterminate
BLM	CNA-Seq	DNA-Tumor	Amplification Not Detected
BLM	Seq	DNA-Tumor	Mutation Not Detected
BMPR1A	CNA-Seq	DNA-Tumor	Amplification Not Detected
BMPR1A	Seq	DNA-Tumor	Mutation Not Detected
BRAF	CNA-Seq	DNA-Tumor	Amplification Not Detected
BRAF	Seq	DNA-Tumor	Mutation Not Detected
BRAF	Seq	RNA-Tumor	Fusion Not Detected
BRCA1	CNA-Seq	DNA-Tumor	Amplification Not Detected
BRCA1	Seq	DNA-Tumor	Mutation Not Detected
BRCA2	CNA-Seq	DNA-Tumor	Amplification Not Detected
BRCA2	Seq	DNA-Tumor	Mutation Not Detected
BRD3	Seq	DNA-Tumor	Mutation Not Detected
BRD3	Seq	RNA-Tumor	Fusion Not Detected
BRD4	Seq	DNA-Tumor	Mutation Not Detected
BRD4	Seq	RNA-Tumor	Fusion Not Detected
BRIP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
BRIP1	Seq	DNA-Tumor	Mutation Not Detected
BTG1	Seq	DNA-Tumor	Mutation Not Detected
BTG2	Seq	DNA-Tumor	Mutation Not Detected
BTK	Seq	DNA-Tumor	Mutation Not Detected
BUB1B	Seq	DNA-Tumor	Mutation Not Detected
C15orf65	Seq	DNA-Tumor	Mutation Not Detected
CACNA1D	CNA-Seq	DNA-Tumor	Amplification Not Detected
CACNA1D	Seq	DNA-Tumor	Mutation Not Detected
CALR	CNA-Seq	DNA-Tumor	Amplification Not Detected
CALR	Seq	DNA-Tumor	Mutation Not Detected
CAMTA1	CNA-Seq	DNA-Tumor	Amplification Not Detected
CAMTA1	Seq	DNA-Tumor	Indeterminate
CARD11	CNA-Seq	DNA-Tumor	Amplification Not Detected
CARD11	Seq	DNA-Tumor	Mutation Not Detected
CARS	CNA-Seq	DNA-Tumor	Amplification Not Detected
CARS	Seq	DNA-Tumor	Mutation Not Detected
CASP8	CNA-Seq	DNA-Tumor	Amplification Not Detected
CASP8	Seq	DNA-Tumor	Mutation Not Detected
CBFA2T3	Seq	DNA-Tumor	Mutation Not Detected
CBFB	CNA-Seq	DNA-Tumor	Amplification Not Detected
CBFB	Seq	DNA-Tumor	Mutation Not Detected
CBL	CNA-Seq	DNA-Tumor	Amplification Not Detected
CBL	Seq	DNA-Tumor	Mutation Not Detected
CBLB	Seq	DNA-Tumor	Mutation Not Detected
CCDC6	CNA-Seq	DNA-Tumor	Amplification Not Detected
CCDC6	Seq	DNA-Tumor	Mutation Not Detected
CCND1	CNA-Seq	DNA-Tumor	Amplification Not Detected
CCND1	Seq	DNA-Tumor	Mutation Not Detected
CCND2	CNA-Seq	DNA-Tumor	Amplification Not Detected
CCND2	Seq	DNA-Tumor	Mutation Not Detected
CCND3	CNA-Seq	DNA-Tumor	Amplification Not Detected
CCND3	Seq	DNA-Tumor	Mutation Not Detected
CCNE1	CNA-Seq	DNA-Tumor	Intermediate
CCNE1	Seq	DNA-Tumor	Mutation Not Detected
CD22	Seq	DNA-Tumor	Mutation Not Detected
CD274 (PD-L1)	CNA-Seq	DNA-Tumor	Amplification Not Detected
CD274 (PD-L1)	Seq	DNA-Tumor	Mutation Not Detected
CD70	Seq	DNA-Tumor	Mutation Not Detected
CD74	CNA-Seq	DNA-Tumor	Amplification Not Detected
CD74	Seq	DNA-Tumor	Mutation Not Detected
CD79A	CNA-Seq	DNA-Tumor	Amplification Not Detected
CD79A	Seq	DNA-Tumor	Mutation Not Detected
CD79B	Seq	DNA-Tumor	Mutation Not Detected
CDC73	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDC73	Seq	DNA-Tumor	Mutation Not Detected
CDH1	Seq	DNA-Tumor	Mutation Not Detected
CDH11	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDH11	Seq	DNA-Tumor	Mutation Not Detected
CDH23	Seq	DNA-Tumor	Mutation Not Detected
CDK12	Seq	DNA-Tumor	Mutation Not Detected
CDK4	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDK4	Seq	DNA-Tumor	Mutation Not Detected
CDK6	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDK6	Seq	DNA-Tumor	Mutation Not Detected
CDK8	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDK8	Seq	DNA-Tumor	Mutation Not Detected
CDKN1A	Seq	DNA-Tumor	Mutation Not Detected
CDKN1B	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDKN1B	Seq	DNA-Tumor	Mutation Not Detected
CDKN1C	Seq	DNA-Tumor	Mutation Not Detected
CDKN2A	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDKN2A	Seq	DNA-Tumor	Mutation Not Detected
CDKN2B	Seq	DNA-Tumor	Mutation Not Detected
CDKN2C	Seq	DNA-Tumor	Mutation Not Detected
CDX2	CNA-Seq	DNA-Tumor	Amplification Not Detected
CDX2	Seq	DNA-Tumor	Mutation Not Detected
CEBPA	Seq	DNA-Tumor	Mutation Not Detected
CHD2	Seq	DNA-Tumor	Indeterminate
CHD4	Seq	DNA-Tumor	Mutation Not Detected
CHEK1	CNA-Seq	DNA-Tumor	Amplification Not Detected
CHEK1	Seq	DNA-Tumor	Mutation Not Detected
CHEK2	CNA-Seq	DNA-Tumor	Amplification Not Detected
CHEK2	Seq	DNA-Tumor	Mutation Not Detected
CHIC2	CNA-Seq	DNA-Tumor	Amplification Not Detected
CHIC2	Seq	DNA-Tumor	Mutation Not Detected
CHN1	Seq	DNA-Tumor	Indeterminate
CIC	CNA-Seq	DNA-Tumor	Amplification Not Detected
CIC	Seq	DNA-Tumor	Unclassified Variant	Exon 11 | p.R934L	55
CIITA	Seq	DNA-Tumor	Indeterminate
CLTCL1	CNA-Seq	DNA-Tumor	Amplification Not Detected
CLTCL1	Seq	DNA-Tumor	Mutation Not Detected
CLYBL	Seq	DNA-Tumor	Mutation Not Detected
CNBP	CNA-Seq	DNA-Tumor	Amplification Not Detected
CNBP	Seq	DNA-Tumor	Mutation Not Detected
CNOT3	Seq	DNA-Tumor	Mutation Not Detected
COX6C	Seq	DNA-Tumor	Mutation Not Detected
CREB1	Seq	DNA-Tumor	Mutation Not Detected
CREB3L1	Seq	DNA-Tumor	Mutation Not Detected
CREB3L2	CNA-Seq	DNA-Tumor	Amplification Not Detected
CREB3L2	Seq	DNA-Tumor	Unclassified Variant	Exon 11 | p.V488M	5
CREBBP	CNA-Seq	DNA-Tumor	Amplification Not Detected
CREBBP	Seq	DNA-Tumor	Mutation Not Detected
CRKL	CNA-Seq	DNA-Tumor	Amplification Not Detected
CRKL	Seq	DNA-Tumor	Mutation Not Detected
CRLF2	Seq	DNA-Tumor	Indeterminate
CRTC1	Seq	DNA-Tumor	Mutation Not Detected
CRTC3	CNA-Seq	DNA-Tumor	Amplification Not Detected
CRTC3	Seq	DNA-Tumor	Mutation Not Detected
CSF1R	CNA-Seq	DNA-Tumor	Amplification Not Detected
CSF1R	Seq	DNA-Tumor	Mutation Not Detected
CSF3R	Seq	DNA-Tumor	Mutation Not Detected
CTCF	CNA-Seq	DNA-Tumor	Amplification Not Detected
CTCF	Seq	DNA-Tumor	Mutation Not Detected
CTLA4	Seq	DNA-Tumor	Mutation Not Detected
CTNNA1	CNA-Seq	DNA-Tumor	Amplification Not Detected
CTNNA1	Seq	DNA-Tumor	Mutation Not Detected
CTNNB1	CNA-Seq	DNA-Tumor	Amplification Not Detected
CTNNB1	Seq	DNA-Tumor	Mutation Not Detected
CUL3	Seq	DNA-Tumor	Mutation Not Detected
CUL4A	Seq	DNA-Tumor	Mutation Not Detected
CUX1	Seq	DNA-Tumor	Mutation Not Detected
CXCR4	Seq	DNA-Tumor	Mutation Not Detected
CYLD	CNA-Seq	DNA-Tumor	Amplification Not Detected
CYLD	Seq	DNA-Tumor	Mutation Not Detected
CYP17A1	Seq	DNA-Tumor	Mutation Not Detected
CYP2D6	Seq	DNA-Tumor	Mutation Not Detected
DAXX	CNA-Seq	DNA-Tumor	Amplification Not Detected
DAXX	Seq	DNA-Tumor	Mutation Not Detected
DDB2	Seq	DNA-Tumor	Mutation Not Detected
DDIT3	Seq	DNA-Tumor	Mutation Not Detected
DDR1	Seq	DNA-Tumor	Indeterminate
DDR2	CNA-Seq	DNA-Tumor	Amplification Not Detected
DDR2	Seq	DNA-Tumor	Mutation Not Detected
DDX3X	Seq	DNA-Tumor	Mutation Not Detected
DDX41	Seq	DNA-Tumor	Mutation Not Detected
DDX6	CNA-Seq	DNA-Tumor	Amplification Not Detected
DDX6	Seq	DNA-Tumor	Mutation Not Detected
DEK	CNA-Seq	DNA-Tumor	Amplification Not Detected
DEK	Seq	DNA-Tumor	Indeterminate
DICER1	CNA-Seq	DNA-Tumor	Amplification Not Detected
DICER1	Seq	DNA-Tumor	Mutation Not Detected
DIS3	Seq	DNA-Tumor	Mutation Not Detected
DIS3L2	Seq	DNA-Tumor	Mutation Not Detected
DKC1	Seq	DNA-Tumor	Mutation Not Detected
DMC1	Seq	DNA-Tumor	Mutation Not Detected
DNA2	Seq	DNA-Tumor	Indeterminate
DNAJB1	Seq	DNA-Tumor	Mutation Not Detected
DNMT3A	Seq	DNA-Tumor	Mutation Not Detected
DOT1L	Seq	DNA-Tumor	Indeterminate
EBF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
EBF1	Seq	DNA-Tumor	Mutation Not Detected
ECT2L	CNA-Seq	DNA-Tumor	Amplification Not Detected
ECT2L	Seq	DNA-Tumor	Mutation Not Detected
EED	Seq	DNA-Tumor	Mutation Not Detected
EGFR	CNA-Seq	DNA-Tumor	Amplification Not Detected
EGFR	Seq	DNA-Tumor	Mutation Not Detected
EGFR	Seq	RNA-Tumor	Fusion Not Detected
EGFRvIII	Seq	RNA-Tumor	Variant Transcript Not Detected
EGLN1	Seq	DNA-Tumor	Mutation Not Detected
EIF1AX	Seq	DNA-Tumor	Mutation Not Detected
EIF4A2	Seq	DNA-Tumor	Mutation Not Detected
ELF3	Seq	DNA-Tumor	Mutation Not Detected
ELK4	CNA-Seq	DNA-Tumor	Amplification Not Detected
ELK4	Seq	DNA-Tumor	Mutation Not Detected
ELOC	Seq	DNA-Tumor	Indeterminate
EME1	Seq	DNA-Tumor	Mutation Not Detected
EME2	Seq	DNA-Tumor	Mutation Not Detected
EML4	Seq	DNA-Tumor	Indeterminate
EMSY	Seq	DNA-Tumor	Mutation Not Detected
ENAH	Seq	RNA-Tumor	Unclassified Fusion Detected	ENAH:ACBD3 |
EP300	CNA-Seq	DNA-Tumor	Amplification Not Detected
EP300	Seq	DNA-Tumor	Mutation Not Detected
EPCAM	Seq	DNA-Tumor	Mutation Not Detected
EPHA2	Seq	DNA-Tumor	Mutation Not Detected
EPHA3	CNA-Seq	DNA-Tumor	Amplification Not Detected
EPHA3	Seq	DNA-Tumor	Mutation Not Detected
EPHA5	Seq	DNA-Tumor	Mutation Not Detected
EPHA7	Seq	DNA-Tumor	Mutation Not Detected
EPHB1	Seq	DNA-Tumor	Mutation Not Detected
EPHB4	Seq	DNA-Tumor	Mutation Not Detected
ER	IHC	Protein	Positive | 1+, 10%
ERBB2 (Her2/Neu)	CISH	DNA-Tumor	Amplified
ERBB2 (Her2/Neu)	CNA-Seq	DNA-Tumor	Intermediate
ERBB2 (Her2/Neu)	IHC	Protein	Positive | 3+, 20%
ERBB2 (Her2/Neu)	Seq	DNA-Tumor	Mutation Not Detected
ERBB3	CNA-Seq	DNA-Tumor	Amplification Not Detected
ERBB3	Seq	DNA-Tumor	Mutation Not Detected
ERBB4	CNA-Seq	DNA-Tumor	Amplification Not Detected
ERBB4	Seq	DNA-Tumor	Mutation Not Detected
ERC1	Seq	DNA-Tumor	Mutation Not Detected
ERCC1	Seq	DNA-Tumor	Mutation Not Detected
ERCC2	CNA-Seq	DNA-Tumor	Amplification Not Detected
ERCC2	Seq	DNA-Tumor	Mutation Not Detected
ERCC3	CNA-Seq	DNA-Tumor	Amplification Not Detected
ERCC3	Seq	DNA-Tumor	Mutation Not Detected
ERCC4	Seq	DNA-Tumor	Mutation Not Detected
ERCC5	Seq	DNA-Tumor	Mutation Not Detected
ERCC6	Seq	DNA-Tumor	Mutation Not Detected
EREG	Seq	DNA-Tumor	Mutation Not Detected
ERG	CNA-Seq	DNA-Tumor	Amplification Not Detected
ERG	Seq	DNA-Tumor	Mutation Not Detected
ERG	Seq	RNA-Tumor	Fusion Not Detected
ERRFI1	Seq	DNA-Tumor	Mutation Not Detected
ESR1	CNA-Seq	DNA-Tumor	Amplification Not Detected
ESR1	Seq	DNA-Tumor	Mutation Not Detected
ESR1	Seq	RNA-Tumor	Fusion Not Detected
ETS1	Seq	DNA-Tumor	Mutation Not Detected
ETV1	CNA-Seq	DNA-Tumor	Amplification Not Detected
ETV1	Seq	DNA-Tumor	Mutation Not Detected
ETV1	Seq	RNA-Tumor	Fusion Not Detected
ETV4	Seq	DNA-Tumor	Mutation Not Detected
ETV4	Seq	RNA-Tumor	Fusion Not Detected
ETV5	CNA-Seq	DNA-Tumor	Amplification Not Detected
ETV5	Seq	DNA-Tumor	Mutation Not Detected
ETV5	Seq	RNA-Tumor	Fusion Not Detected
ETV6	CNA-Seq	DNA-Tumor	Amplification Not Detected
ETV6	Seq	DNA-Tumor	Mutation Not Detected
ETV6	Seq	RNA-Tumor	Fusion Not Detected
EWSR1	CNA-Seq	DNA-Tumor	Amplification Not Detected
EWSR1	Seq	DNA-Tumor	Mutation Not Detected
EWSR1	Seq	RNA-Tumor	Fusion Not Detected
EXO1	Seq	DNA-Tumor	Mutation Not Detected
EXT1	CNA-Seq	DNA-Tumor	Amplification Not Detected
EXT1	Seq	DNA-Tumor	Mutation Not Detected
EXT2	CNA-Seq	DNA-Tumor	Amplification Not Detected
EXT2	Seq	DNA-Tumor	Mutation Not Detected
EZH2	CNA-Seq	DNA-Tumor	Amplification Not Detected
EZH2	Seq	DNA-Tumor	Mutation Not Detected
EZR	CNA-Seq	DNA-Tumor	Amplification Not Detected
EZR	Seq	DNA-Tumor	Mutation Not Detected
FAM46C	Seq	DNA-Tumor	Mutation Not Detected
FANCA	CNA-Seq	DNA-Tumor	Amplification Not Detected
FANCA	Seq	DNA-Tumor	Mutation Not Detected
FANCB	Seq	DNA-Tumor	Mutation Not Detected
FANCC	CNA-Seq	DNA-Tumor	Amplification Not Detected
FANCC	Seq	DNA-Tumor	Mutation Not Detected
FANCD2	CNA-Seq	DNA-Tumor	Amplification Not Detected
FANCD2	Seq	DNA-Tumor	Variant of Uncertain Significance	Exon 3 | p.Q26H	39
FANCE	CNA-Seq	DNA-Tumor	Amplification Not Detected
FANCE	Seq	DNA-Tumor	Mutation Not Detected
FANCF	Seq	DNA-Tumor	Mutation Not Detected
FANCG	CNA-Seq	DNA-Tumor	Amplification Not Detected
FANCG	Seq	DNA-Tumor	Mutation Not Detected
FANCI	Seq	DNA-Tumor	Mutation Not Detected
FANCL	CNA-Seq	DNA-Tumor	Amplification Not Detected
FANCL	Seq	DNA-Tumor	Mutation Not Detected
FANCM	Seq	DNA-Tumor	Mutation Not Detected
FAS	CNA-Seq	DNA-Tumor	Amplification Not Detected
FAS	Seq	DNA-Tumor	Mutation Not Detected
FAT1	Seq	DNA-Tumor	Mutation Not Detected
FAT3	Seq	DNA-Tumor	Mutation Not Detected
FBXO11	Seq	DNA-Tumor	Mutation Not Detected
FBXW7	CNA-Seq	DNA-Tumor	Amplification Not Detected
FBXW7	Seq	DNA-Tumor	Mutation Not Detected
FCRL4	CNA-Seq	DNA-Tumor	Amplification Not Detected
FCRL4	Seq	DNA-Tumor	Mutation Not Detected
FEN1	Seq	DNA-Tumor	Mutation Not Detected
FGF10	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGF10	Seq	DNA-Tumor	Mutation Not Detected
FGF12	Seq	DNA-Tumor	Mutation Not Detected
FGF14	Seq	DNA-Tumor	Mutation Not Detected
FGF19	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGF19	Seq	DNA-Tumor	Mutation Not Detected
FGF23	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGF23	Seq	DNA-Tumor	Mutation Not Detected
FGF3	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGF3	Seq	DNA-Tumor	Mutation Not Detected
FGF4	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGF4	Seq	DNA-Tumor	Mutation Not Detected
FGF6	Seq	DNA-Tumor	Mutation Not Detected
FGFR1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGFR1	Seq	DNA-Tumor	Mutation Not Detected
FGFR1	Seq	RNA-Tumor	Fusion Not Detected
FGFR1OP	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGFR1OP	Seq	DNA-Tumor	Mutation Not Detected
FGFR2	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGFR2	Seq	DNA-Tumor	Mutation Not Detected
FGFR2	Seq	RNA-Tumor	Fusion Not Detected
FGFR3	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGFR3	Seq	DNA-Tumor	Mutation Not Detected
FGFR3	Seq	RNA-Tumor	Fusion Not Detected
FGFR4	CNA-Seq	DNA-Tumor	Amplification Not Detected
FGFR4	Seq	DNA-Tumor	Mutation Not Detected
FGR	Seq	RNA-Tumor	Fusion Not Detected
FH	CNA-Seq	DNA-Tumor	Amplification Not Detected
FH	Seq	DNA-Tumor	Mutation Not Detected
FHIT	CNA-Seq	DNA-Tumor	Amplification Not Detected
FHIT	Seq	DNA-Tumor	Mutation Not Detected
FIP1L1	Seq	DNA-Tumor	Mutation Not Detected
FLCN	CNA-Seq	DNA-Tumor	Amplification Not Detected
FLCN	Seq	DNA-Tumor	Mutation Not Detected
FLI1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FLI1	Seq	DNA-Tumor	Mutation Not Detected
FLT1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FLT1	Seq	DNA-Tumor	Mutation Not Detected
FLT3	CNA-Seq	DNA-Tumor	Amplification Not Detected
FLT3	Seq	DNA-Tumor	Mutation Not Detected
FLT4	CNA-Seq	DNA-Tumor	Amplification Not Detected
FLT4	Seq	DNA-Tumor	Mutation Not Detected
FNBP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FNBP1	Seq	DNA-Tumor	Mutation Not Detected
FOXA1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FOXA1	Seq	DNA-Tumor	Mutation Not Detected
FOXL2	Seq	DNA-Tumor	Mutation Not Detected
FOXO1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FOXO1	Seq	DNA-Tumor	Mutation Not Detected
FOXO3	Seq	DNA-Tumor	Mutation Not Detected
FOXO4	Seq	DNA-Tumor	Mutation Not Detected
FOXP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FOXP1	Seq	DNA-Tumor	Mutation Not Detected
FRS2	Seq	DNA-Tumor	Mutation Not Detected
FSTL3	Seq	DNA-Tumor	Mutation Not Detected
FUBP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
FUBP1	Seq	DNA-Tumor	Mutation Not Detected
FUS	CNA-Seq	DNA-Tumor	Amplification Not Detected
FUS	Seq	DNA-Tumor	Mutation Not Detected
FYN	Seq	DNA-Tumor	Mutation Not Detected
GABRA6	Seq	DNA-Tumor	Mutation Not Detected
GALNT12	Seq	DNA-Tumor	Mutation Not Detected
GATA1	Seq	DNA-Tumor	Mutation Not Detected
GATA2	Seq	DNA-Tumor	Mutation Not Detected
GATA3	CNA-Seq	DNA-Tumor	Amplification Not Detected
GATA3	Seq	DNA-Tumor	Mutation Not Detected
GATA4	Seq	DNA-Tumor	Mutation Not Detected
GATA6	Seq	DNA-Tumor	Mutation Not Detected
GEN1	Seq	DNA-Tumor	Indeterminate
Genomic LOH	Seq	DNA-Tumor	Low		0
GID4	CNA-Seq	DNA-Tumor	Amplification Not Detected
GID4	Seq	DNA-Tumor	Mutation Not Detected
GLI1	Seq	DNA-Tumor	Indeterminate
GLI2	Seq	DNA-Tumor	Mutation Not Detected
GMPS	CNA-Seq	DNA-Tumor	Amplification Not Detected
GMPS	Seq	DNA-Tumor	Unclassified Variant	Exon 9 | p.G367S	58
GNA11	Seq	DNA-Tumor	Mutation Not Detected
GNA13	CNA-Seq	DNA-Tumor	Amplification Not Detected
GNA13	Seq	DNA-Tumor	Mutation Not Detected
GNAQ	CNA-Seq	DNA-Tumor	Amplification Not Detected
GNAQ	Seq	DNA-Tumor	Mutation Not Detected
GNAS	CNA-Seq	DNA-Tumor	Amplification Not Detected
GNAS	Seq	DNA-Tumor	Mutation Not Detected
GOPC	Seq	DNA-Tumor	Indeterminate
GPC3	Seq	DNA-Tumor	Mutation Not Detected
GPS2	Seq	DNA-Tumor	Mutation Not Detected
GREM1	Seq	DNA-Tumor	Mutation Not Detected
GRIN2A	CNA-Seq	DNA-Tumor	Amplification Not Detected
GRIN2A	Seq	DNA-Tumor	Mutation Not Detected
GRM3	Seq	DNA-Tumor	Mutation Not Detected
GSK3B	Seq	DNA-Tumor	Mutation Not Detected
H2AFX	Seq	DNA-Tumor	Mutation Not Detected
H3F3A	CNA-Seq	DNA-Tumor	Amplification Not Detected
H3F3A	Seq	DNA-Tumor	Mutation Not Detected
H3F3B	CNA-Seq	DNA-Tumor	Amplification Not Detected
H3F3B	Seq	DNA-Tumor	Mutation Not Detected
HDAC1	Seq	DNA-Tumor	Mutation Not Detected
HEY1	Seq	DNA-Tumor	Mutation Not Detected
HGF	Seq	DNA-Tumor	Mutation Not Detected
HIF1A	Seq	DNA-Tumor	Mutation Not Detected
HIST1H3B	Seq	DNA-Tumor	Mutation Not Detected
HIST1H3C	Seq	DNA-Tumor	Mutation Not Detected
HIST1H4I	Seq	DNA-Tumor	Mutation Not Detected
HLF	Seq	DNA-Tumor	Mutation Not Detected
HMGA2	CNA-Seq	DNA-Tumor	Amplification Not Detected
HMGA2	Seq	DNA-Tumor	Mutation Not Detected
HMGN2P46	Seq	DNA-Tumor	Indeterminate
HNF1A	Seq	DNA-Tumor	Mutation Not Detected
HOOK3	CNA-Seq	DNA-Tumor	Amplification Not Detected
HOOK3	Seq	DNA-Tumor	Mutation Not Detected
HOXA11	Seq	DNA-Tumor	Mutation Not Detected
HOXA13	Seq	DNA-Tumor	Mutation Not Detected
HOXA9	Seq	DNA-Tumor	Mutation Not Detected
HOXB13	Seq	DNA-Tumor	Mutation Not Detected
HOXD13	Seq	DNA-Tumor	Mutation Not Detected
HRAS	Seq	DNA-Tumor	Mutation Not Detected
HRG	Seq	DNA-Tumor	Mutation Not Detected
HSD3B1	Seq	DNA-Tumor	Mutation Not Detected
HSP90AA1	CNA-Seq	DNA-Tumor	Amplification Not Detected
HSP90AA1	Seq	DNA-Tumor	Mutation Not Detected
HSP90AB1	CNA-Seq	DNA-Tumor	Amplification Not Detected
HSP90AB1	Seq	DNA-Tumor	Mutation Not Detected
ID2	Seq	DNA-Tumor	Mutation Not Detected
ID3	Seq	DNA-Tumor	Mutation Not Detected
IDH1	CNA-Seq	DNA-Tumor	Amplification Not Detected
IDH1	Seq	DNA-Tumor	Mutation Not Detected
IDH2	CNA-Seq	DNA-Tumor	Amplification Not Detected
IDH2	Seq	DNA-Tumor	Mutation Not Detected
IFNGR1	Seq	DNA-Tumor	Mutation Not Detected
IGF1R	CNA-Seq	DNA-Tumor	Amplification Not Detected
IGF1R	Seq	DNA-Tumor	Mutation Not Detected
IGF2	Seq	DNA-Tumor	Mutation Not Detected
IKBKE	Seq	DNA-Tumor	Unclassified Variant	Exon 10 | p.S377N	45
IKZF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
IKZF1	Seq	DNA-Tumor	Mutation Not Detected
IL7R	CNA-Seq	DNA-Tumor	Amplification Not Detected
IL7R	Seq	DNA-Tumor	Mutation Not Detected
INHBA	Seq	DNA-Tumor	Mutation Not Detected
INPP4B	Seq	DNA-Tumor	Mutation Not Detected
INSR	Seq	RNA-Tumor	Fusion Not Detected
IRF1	Seq	DNA-Tumor	Mutation Not Detected
IRF2	Seq	DNA-Tumor	Mutation Not Detected
IRF4	CNA-Seq	DNA-Tumor	Amplification Not Detected
IRF4	Seq	DNA-Tumor	Mutation Not Detected
IRS2	Seq	DNA-Tumor	Mutation Not Detected
ITK	CNA-Seq	DNA-Tumor	Amplification Not Detected
ITK	Seq	DNA-Tumor	Mutation Not Detected
JAK1	CNA-Seq	DNA-Tumor	Amplification Not Detected
JAK1	Seq	DNA-Tumor	Mutation Not Detected
JAK2	CNA-Seq	DNA-Tumor	Amplification Not Detected
JAK2	Seq	DNA-Tumor	Mutation Not Detected
JAK3	CNA-Seq	DNA-Tumor	Amplification Not Detected
JAK3	Seq	DNA-Tumor	Mutation Not Detected
JAZF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
JAZF1	Seq	DNA-Tumor	Mutation Not Detected
JUN	Seq	DNA-Tumor	Mutation Not Detected
KAT6A	Seq	DNA-Tumor	Mutation Not Detected
KAT6B	Seq	DNA-Tumor	Mutation Not Detected
KCNJ5	Seq	DNA-Tumor	Mutation Not Detected
KDM5A	Seq	DNA-Tumor	Mutation Not Detected
KDM5C	Seq	DNA-Tumor	Mutation Not Detected
KDM6A	Seq	DNA-Tumor	Mutation Not Detected
KDR (VEGFR2)	CNA-Seq	DNA-Tumor	Amplification Not Detected
KDR (VEGFR2)	Seq	DNA-Tumor	Mutation Not Detected
KDSR	Seq	DNA-Tumor	Mutation Not Detected
KEAP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
KEAP1	Seq	DNA-Tumor	Mutation Not Detected
KEL	Seq	DNA-Tumor	Mutation Not Detected
KIAA1549	CNA-Seq	DNA-Tumor	Amplification Not Detected
KIAA1549	Seq	DNA-Tumor	Indeterminate
KIF1B	Seq	DNA-Tumor	Mutation Not Detected
KIF5B	CNA-Seq	DNA-Tumor	Amplification Not Detected
KIF5B	Seq	DNA-Tumor	Mutation Not Detected
KIT	CNA-Seq	DNA-Tumor	Amplification Not Detected
KIT	Seq	DNA-Tumor	Mutation Not Detected
KLF4	Seq	DNA-Tumor	Mutation Not Detected
KLHL6	CNA-Seq	DNA-Tumor	Amplification Not Detected
KLHL6	Seq	DNA-Tumor	Mutation Not Detected
KLK2	Seq	DNA-Tumor	Mutation Not Detected
KMT2A	CNA-Seq	DNA-Tumor	Amplification Not Detected
KMT2A	Seq	DNA-Tumor	Mutation Not Detected
KMT2C	CNA-Seq	DNA-Tumor	Amplification Not Detected
KMT2C	Seq	DNA-Tumor	Mutation Not Detected
KMT2D	CNA-Seq	DNA-Tumor	Amplification Not Detected
KMT2D	Seq	DNA-Tumor	Mutation Not Detected
KRAS	CNA-Seq	DNA-Tumor	Amplification Not Detected
KRAS	Seq	DNA-Tumor	Pathogenic Variant	Exon 2 | p.G13C	35
LCK	CNA-Seq	DNA-Tumor	Amplification Not Detected
LCK	Seq	DNA-Tumor	Mutation Not Detected
LDLR	Seq	DNA-Tumor	Mutation Not Detected
LHFPL6	CNA-Seq	DNA-Tumor	Amplification Not Detected
LHFPL6	Seq	DNA-Tumor	Mutation Not Detected
LIFR	CNA-Seq	DNA-Tumor	Amplification Not Detected
LIFR	Seq	DNA-Tumor	Indeterminate
LIG1	Seq	DNA-Tumor	Mutation Not Detected
LMNA	Seq	DNA-Tumor	Mutation Not Detected
LMO1	Seq	DNA-Tumor	Mutation Not Detected
LMO2	Seq	DNA-Tumor	Indeterminate
LPP	CNA-Seq	DNA-Tumor	Amplification Not Detected
LPP	Seq	DNA-Tumor	Mutation Not Detected
LRP1B	CNA-Seq	DNA-Tumor	Amplification Not Detected
LRP1B	Seq	DNA-Tumor	Mutation Not Detected
LTK	Seq	DNA-Tumor	Indeterminate
LYN	Seq	DNA-Tumor	Mutation Not Detected
LZTR1	Seq	DNA-Tumor	Mutation Not Detected
MAF	CNA-Seq	DNA-Tumor	Amplification Not Detected
MAF	Seq	DNA-Tumor	Mutation Not Detected
MAFB	Seq	DNA-Tumor	Mutation Not Detected
MAGI2	Seq	DNA-Tumor	Indeterminate
MALT1	Seq	DNA-Tumor	Indeterminate
MAML2	CNA-Seq	DNA-Tumor	Amplification Not Detected
MAML2	Seq	DNA-Tumor	Mutation Not Detected
MAML2	Seq	RNA-Tumor	Fusion Not Detected
MAP2K1 (MEK1)	CNA-Seq	DNA-Tumor	Amplification Not Detected
MAP2K1 (MEK1)	Seq	DNA-Tumor	Mutation Not Detected
MAP2K2 (MEK2)	CNA-Seq	DNA-Tumor	Amplification Not Detected
MAP2K2 (MEK2)	Seq	DNA-Tumor	Mutation Not Detected
MAP2K4	CNA-Seq	DNA-Tumor	Amplification Not Detected
MAP2K4	Seq	DNA-Tumor	Mutation Not Detected
MAP3K1	CNA-Seq	DNA-Tumor	Amplification Not Detected
MAP3K1	Seq	DNA-Tumor	Mutation Not Detected
MAP3K13	Seq	DNA-Tumor	Mutation Not Detected
MAPK1	Seq	DNA-Tumor	Mutation Not Detected
MAPK3	Seq	DNA-Tumor	Mutation Not Detected
MAST1	Seq	RNA-Tumor	Fusion Not Detected
MAST2	Seq	RNA-Tumor	Fusion Not Detected
MAX	Seq	DNA-Tumor	Mutation Not Detected
MBD4	Seq	DNA-Tumor	Mutation Not Detected
MCL1	CNA-Seq	DNA-Tumor	Amplification Not Detected
MCL1	Seq	DNA-Tumor	Mutation Not Detected
MDC1	Seq	DNA-Tumor	Mutation Not Detected
MDM2	CNA-Seq	DNA-Tumor	Amplification Not Detected
MDM2	Seq	DNA-Tumor	Mutation Not Detected
MDM4	CNA-Seq	DNA-Tumor	Amplification Not Detected
MDM4	Seq	DNA-Tumor	Mutation Not Detected
MDS2	CNA-Seq	DNA-Tumor	Amplification Not Detected
MDS2	Seq	DNA-Tumor	Mutation Not Detected
MECOM	Seq	DNA-Tumor	Mutation Not Detected
MED12	Seq	DNA-Tumor	Indeterminate
MEF2B	CNA-Seq	DNA-Tumor	Amplification Not Detected
MEF2B	Seq	DNA-Tumor	Mutation Not Detected
MEN1	CNA-Seq	DNA-Tumor	Amplification Not Detected
MEN1	Seq	DNA-Tumor	Mutation Not Detected
MERTK	Seq	DNA-Tumor	Mutation Not Detected
MET	CNA-Seq	DNA-Tumor	Amplification Not Detected
MET	Seq	DNA-Tumor	Mutation Not Detected
MET	Seq	RNA-Tumor	Fusion Not Detected
MET	Seq	RNA-Tumor	Variant Transcript Not Detected
MGA	Seq	DNA-Tumor	Mutation Not Detected
MGMT	Seq	DNA-Tumor	Mutation Not Detected
Mismatch Repair Status	IHC	Protein	Proficient
MITF	CNA-Seq	DNA-Tumor	Amplification Not Detected
MITF	Seq	DNA-Tumor	Mutation Not Detected
MKNK1	Seq	DNA-Tumor	Indeterminate
MLF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
MLF1	Seq	DNA-Tumor	Mutation Not Detected
MLH1	CNA-Seq	DNA-Tumor	Amplification Not Detected
MLH1	IHC	Protein	Positive | 1+, 80%
MLH1	Seq	DNA-Tumor	Mutation Not Detected
MLH3	Seq	DNA-Tumor	Unclassified Variant	Exon 2 | p.Q563R	48
MLLT10	CNA-Seq	DNA-Tumor	Amplification Not Detected
MLLT10	Seq	DNA-Tumor	Mutation Not Detected
MLLT11	Seq	DNA-Tumor	Mutation Not Detected
MLLT3	CNA-Seq	DNA-Tumor	Amplification Not Detected
MLLT3	Seq	DNA-Tumor	Mutation Not Detected
MN1	Seq	DNA-Tumor	Mutation Not Detected
MPL	Seq	DNA-Tumor	Mutation Not Detected
MRE11	CNA-Seq	DNA-Tumor	Amplification Not Detected
MRE11	Seq	DNA-Tumor	Mutation Not Detected
MSH2	CNA-Seq	DNA-Tumor	Amplification Not Detected
MSH2	IHC	Protein	Positive | 2+, 95%
MSH2	Seq	DNA-Tumor	Mutation Not Detected
MSH3	Seq	DNA-Tumor	Mutation Not Detected
MSH6	CNA-Seq	DNA-Tumor	Amplification Not Detected
MSH6	IHC	Protein	Positive | 2+, 95%
MSH6	Seq	DNA-Tumor	Mutation Not Detected
MSI	Seq	DNA-Tumor	Stable		0
MSI2	CNA-Seq	DNA-Tumor	Amplification Not Detected
MSI2	Seq	DNA-Tumor	Mutation Not Detected
MSMB	Seq	RNA-Tumor	Fusion Not Detected
MST1R (RON)	Seq	DNA-Tumor	Mutation Not Detected
MTAP	Seq	DNA-Tumor	Mutation Not Detected
MTCP1	Seq	DNA-Tumor	Mutation Not Detected
MTOR	CNA-Seq	DNA-Tumor	Amplification Not Detected
MTOR	Seq	DNA-Tumor	Mutation Not Detected
MUC1	Seq	DNA-Tumor	Mutation Not Detected
MUS81	Seq	DNA-Tumor	Mutation Not Detected
MUSK	Seq	RNA-Tumor	Fusion Not Detected
MUTYH	Seq	DNA-Tumor	Mutation Not Detected
MYB	CNA-Seq	DNA-Tumor	Amplification Not Detected
MYB	Seq	DNA-Tumor	Indeterminate
MYB	Seq	RNA-Tumor	Fusion Not Detected
MYC	CNA-Seq	DNA-Tumor	Amplification Not Detected
MYC	Seq	DNA-Tumor	Mutation Not Detected
MYCL	Seq	DNA-Tumor	Mutation Not Detected
MYCN	CNA-Seq	DNA-Tumor	Amplification Not Detected
MYCN	Seq	DNA-Tumor	Mutation Not Detected
MYD88	CNA-Seq	DNA-Tumor	Amplification Not Detected
MYD88	Seq	DNA-Tumor	Mutation Not Detected
MYH11	Seq	DNA-Tumor	Mutation Not Detected
MYH9	Seq	DNA-Tumor	Mutation Not Detected
NBN	Seq	DNA-Tumor	Mutation Not Detected
NCOA2	CNA-Seq	DNA-Tumor	Amplification Not Detected
NCOA2	Seq	DNA-Tumor	Mutation Not Detected
NCOA3	Seq	DNA-Tumor	Mutation Not Detected
NCOA4	Seq	DNA-Tumor	Mutation Not Detected
NCOR1	Seq	DNA-Tumor	Mutation Not Detected
NDRG1	Seq	DNA-Tumor	Mutation Not Detected
NF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
NF1	Seq	DNA-Tumor	Mutation Not Detected
NF2	CNA-Seq	DNA-Tumor	Amplification Not Detected
NF2	Seq	DNA-Tumor	Mutation Not Detected
NFE2L2	CNA-Seq	DNA-Tumor	Amplification Not Detected
NFE2L2	Seq	DNA-Tumor	Mutation Not Detected
NFIB	CNA-Seq	DNA-Tumor	Amplification Not Detected
NFIB	Seq	DNA-Tumor	Mutation Not Detected
NFKB2	CNA-Seq	DNA-Tumor	Amplification Not Detected
NFKB2	Seq	DNA-Tumor	Mutation Not Detected
NFKBIA	CNA-Seq	DNA-Tumor	Amplification Not Detected
NFKBIA	Seq	DNA-Tumor	Mutation Not Detected
NFKBIE	Seq	DNA-Tumor	Mutation Not Detected
NIN	CNA-Seq	DNA-Tumor	Amplification Not Detected
NIN	Seq	DNA-Tumor	Mutation Not Detected
NKX2-1	Seq	DNA-Tumor	Mutation Not Detected
NONO	Seq	DNA-Tumor	Mutation Not Detected
NOTCH1	Seq	DNA-Tumor	Mutation Not Detected
NOTCH1	Seq	RNA-Tumor	Fusion Not Detected
NOTCH2	CNA-Seq	DNA-Tumor	Amplification Not Detected
NOTCH2	Seq	DNA-Tumor	Mutation Not Detected
NOTCH2	Seq	RNA-Tumor	Fusion Not Detected
NOTCH3	Seq	DNA-Tumor	Indeterminate
NPM1	CNA-Seq	DNA-Tumor	Amplification Not Detected
NPM1	Seq	DNA-Tumor	Indeterminate
NR4A3	CNA-Seq	DNA-Tumor	Amplification Not Detected
NR4A3	Seq	DNA-Tumor	Mutation Not Detected
NRAS	Seq	DNA-Tumor	Mutation Not Detected
NRG1	Seq	RNA-Tumor	Fusion Not Detected
NSD1	CNA-Seq	DNA-Tumor	Amplification Not Detected
NSD1	Seq	DNA-Tumor	Mutation Not Detected
NSD2	Seq	DNA-Tumor	Mutation Not Detected
NSD3	Seq	DNA-Tumor	Mutation Not Detected
NT5C2	Seq	DNA-Tumor	Mutation Not Detected
NTHL1	Seq	DNA-Tumor	Mutation Not Detected
NTRK1	CNA-Seq	DNA-Tumor	Amplification Not Detected
NTRK1	Seq	DNA-Tumor	Mutation Not Detected
NTRK1	Seq	RNA-Tumor	Fusion Not Detected
NTRK2	CNA-Seq	DNA-Tumor	Amplification Not Detected
NTRK2	Seq	DNA-Tumor	Mutation Not Detected
NTRK2	Seq	RNA-Tumor	Fusion Not Detected
NTRK3	CNA-Seq	DNA-Tumor	Amplification Not Detected
NTRK3	Seq	DNA-Tumor	Mutation Not Detected
NTRK3	Seq	RNA-Tumor	Fusion Not Detected
NUMBL	Seq	RNA-Tumor	Fusion Not Detected
NUP214	CNA-Seq	DNA-Tumor	Amplification Not Detected
NUP214	Seq	DNA-Tumor	Mutation Not Detected
NUP93	CNA-Seq	DNA-Tumor	Amplification Not Detected
NUP93	Seq	DNA-Tumor	Mutation Not Detected
NUP98	CNA-Seq	DNA-Tumor	Amplification Not Detected
NUP98	Seq	DNA-Tumor	Mutation Not Detected
NUTM1	CNA-Seq	DNA-Tumor	Amplification Not Detected
NUTM1	Seq	DNA-Tumor	Mutation Not Detected
NUTM1	Seq	RNA-Tumor	Fusion Not Detected
OLIG2	Seq	DNA-Tumor	Mutation Not Detected
P2RY8	Seq	DNA-Tumor	Mutation Not Detected
PAFAH1B2	Seq	DNA-Tumor	Mutation Not Detected
PAK1	Seq	DNA-Tumor	Mutation Not Detected
PAK3	Seq	DNA-Tumor	Mutation Not Detected
PALB2	CNA-Seq	DNA-Tumor	Amplification Not Detected
PALB2	Seq	DNA-Tumor	Mutation Not Detected
PARP1	Seq	DNA-Tumor	Mutation Not Detected
PARP2	Seq	DNA-Tumor	Mutation Not Detected
PARP3	Seq	DNA-Tumor	Mutation Not Detected
PAX3	CNA-Seq	DNA-Tumor	Amplification Not Detected
PAX3	Seq	DNA-Tumor	Mutation Not Detected
PAX5	CNA-Seq	DNA-Tumor	Amplification Not Detected
PAX5	Seq	DNA-Tumor	Mutation Not Detected
PAX7	Seq	DNA-Tumor	Mutation Not Detected
PAX8	Seq	DNA-Tumor	Mutation Not Detected
PBRM1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PBRM1	Seq	DNA-Tumor	Mutation Not Detected
PBX1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PBX1	Seq	DNA-Tumor	Mutation Not Detected
PCM1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PCM1	Seq	DNA-Tumor	Mutation Not Detected
PDCD1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PDCD1	Seq	DNA-Tumor	Mutation Not Detected
PDCD1LG2	CNA-Seq	DNA-Tumor	Amplification Not Detected
PDCD1LG2	Seq	DNA-Tumor	Mutation Not Detected
PDE4DIP	Seq	DNA-Tumor	Mutation Not Detected
PDGFB	Seq	DNA-Tumor	Mutation Not Detected
PDGFRA	CNA-Seq	DNA-Tumor	Amplification Not Detected
PDGFRA	Seq	DNA-Tumor	Mutation Not Detected
PDGFRA	Seq	RNA-Tumor	Fusion Not Detected
PDGFRB	CNA-Seq	DNA-Tumor	Amplification Not Detected
PDGFRB	Seq	DNA-Tumor	Mutation Not Detected
PDGFRB	Seq	RNA-Tumor	Fusion Not Detected
PDK1	Seq	DNA-Tumor	Mutation Not Detected
PD-L1 (SP142)	IHC	Protein	Negative | 0, 0%
PDZD8	Seq	RNA-Tumor	Unclassified Fusion Detected	PDZD8:EFCAB5 |
PER1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PER1	Seq	DNA-Tumor	Mutation Not Detected
PGAP3	Seq	RNA-Tumor	Unclassified Fusion Detected	PGAP3:IKZF3 |
PGAP3	Seq	RNA-Tumor	Unclassified Fusion Detected	PGAP3:IKZF3 |
PHF6	Seq	DNA-Tumor	Mutation Not Detected
PHOX2B	Seq	DNA-Tumor	Mutation Not Detected
PIK3C2B	Seq	DNA-Tumor	Mutation Not Detected
PIK3C2G	Seq	DNA-Tumor	Indeterminate
PIK3CA	CNA-Seq	DNA-Tumor	Amplification Not Detected
PIK3CA	Seq	DNA-Tumor	Pathogenic Variant	Exon 2 | p.R88Q	45
PIK3CA	Seq	RNA-Tumor	Fusion Not Detected
PIK3CB	Seq	DNA-Tumor	Indeterminate
PIK3CD	Seq	DNA-Tumor	Mutation Not Detected
PIK3CG	Seq	DNA-Tumor	Mutation Not Detected
PIK3R1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PIK3R1	Seq	DNA-Tumor	Mutation Not Detected
PIK3R2	Seq	DNA-Tumor	Indeterminate
PIM1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PIM1	Seq	DNA-Tumor	Mutation Not Detected
PITPNC1	Seq	RNA-Tumor	Unclassified Fusion Detected	PITPNC1:CACNG4 |
PKN1	Seq	RNA-Tumor	Fusion Not Detected
PLAG1	Seq	DNA-Tumor	Mutation Not Detected
PLCG2	Seq	DNA-Tumor	Mutation Not Detected
PML	Seq	DNA-Tumor	Mutation Not Detected
PMS1	Seq	DNA-Tumor	Mutation Not Detected
PMS2	CNA-Seq	DNA-Tumor	Amplification Not Detected
PMS2	IHC	Protein	Positive | 1+, 70%
PMS2	Seq	DNA-Tumor	Mutation Not Detected
POLD1	Seq	DNA-Tumor	Mutation Not Detected
POLD2	Seq	DNA-Tumor	Mutation Not Detected
POLD3	Seq	DNA-Tumor	Mutation Not Detected
POLD4	Seq	DNA-Tumor	Mutation Not Detected
POLE	CNA-Seq	DNA-Tumor	Amplification Not Detected
POLE	Seq	DNA-Tumor	Mutation Not Detected
POLH	Seq	DNA-Tumor	Unclassified Variant	Exon 11 | p.E495K	51
POLH	Seq	DNA-Tumor	Unclassified Variant	Exon 4 | p.G153D	48
POLQ	Seq	DNA-Tumor	Mutation Not Detected
POT1	CNA-Seq	DNA-Tumor	Amplification Not Detected
POT1	Seq	DNA-Tumor	Mutation Not Detected
POU2AF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
POU2AF1	Seq	DNA-Tumor	Mutation Not Detected
PPARG	CNA-Seq	DNA-Tumor	Amplification Not Detected
PPARG	Seq	DNA-Tumor	Mutation Not Detected
PPARG	Seq	RNA-Tumor	Fusion Not Detected
PPM1D	Seq	DNA-Tumor	Mutation Not Detected
PPP2R1A	Seq	DNA-Tumor	Mutation Not Detected
PPP2R2A	Seq	DNA-Tumor	Mutation Not Detected
PPP6C	Seq	DNA-Tumor	Mutation Not Detected
PR	IHC	Protein	Negative | 0, 100%
PRCC	CNA-Seq	DNA-Tumor	Amplification Not Detected
PRCC	Seq	DNA-Tumor	Mutation Not Detected
PRDM1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PRDM1	Seq	DNA-Tumor	Mutation Not Detected
PREX2	Seq	DNA-Tumor	Mutation Not Detected
PRF1	Seq	DNA-Tumor	Mutation Not Detected
PRKACA	Seq	DNA-Tumor	Indeterminate
PRKAR1A	CNA-Seq	DNA-Tumor	Amplification Not Detected
PRKAR1A	Seq	DNA-Tumor	Mutation Not Detected
PRKCA	Seq	RNA-Tumor	Fusion Not Detected
PRKCB	Seq	RNA-Tumor	Fusion Not Detected
PRKCH	Seq	DNA-Tumor	Mutation Not Detected
PRKCI	Seq	DNA-Tumor	Mutation Not Detected
PRKDC	Seq	DNA-Tumor	Mutation Not Detected
PRKN	Seq	DNA-Tumor	Mutation Not Detected
PRRX1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PRRX1	Seq	DNA-Tumor	Mutation Not Detected
PRSS8	Seq	DNA-Tumor	Mutation Not Detected
PTCH1	CNA-Seq	DNA-Tumor	Amplification Not Detected
PTCH1	Seq	DNA-Tumor	Mutation Not Detected
PTCH2	Seq	DNA-Tumor	Mutation Not Detected
PTEN	CNA-Seq	DNA-Tumor	Amplification Not Detected
PTEN	IHC	Protein	Positive | 2+, 90%
PTEN	Seq	DNA-Tumor	Mutation Not Detected
PTK2B	Seq	DNA-Tumor	Indeterminate
PTPN11	CNA-Seq	DNA-Tumor	Amplification Not Detected
PTPN11	Seq	DNA-Tumor	Indeterminate
PTPN22	Seq	DNA-Tumor	Indeterminate
PTPRC	CNA-Seq	DNA-Tumor	Amplification Not Detected
PTPRC	Seq	DNA-Tumor	Mutation Not Detected
PTPRD	Seq	DNA-Tumor	Indeterminate
PTPRO	Seq	DNA-Tumor	Indeterminate
PTPRT	Seq	DNA-Tumor	Indeterminate
QKI	Seq	DNA-Tumor	Mutation Not Detected
RABL3	Seq	DNA-Tumor	Mutation Not Detected
RAC1	CNA-Seq	DNA-Tumor	Amplification Not Detected
RAC1	Seq	DNA-Tumor	Indeterminate
RAD21	Seq	DNA-Tumor	Mutation Not Detected
RAD50	CNA-Seq	DNA-Tumor	Amplification Not Detected
RAD50	Seq	DNA-Tumor	Mutation Not Detected
RAD51	Seq	DNA-Tumor	Mutation Not Detected
RAD51B	Seq	DNA-Tumor	Mutation Not Detected
RAD51C	Seq	DNA-Tumor	Mutation Not Detected
RAD51D	Seq	DNA-Tumor	Mutation Not Detected
RAD52	Seq	DNA-Tumor	Mutation Not Detected
RAD54B	Seq	DNA-Tumor	Mutation Not Detected
RAD54L	Seq	DNA-Tumor	Mutation Not Detected
RAF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
RAF1	Seq	DNA-Tumor	Mutation Not Detected
RAF1	Seq	RNA-Tumor	Fusion Not Detected
RANBP2	Seq	DNA-Tumor	Mutation Not Detected
RARA	Seq	DNA-Tumor	Mutation Not Detected
RASA1	Seq	DNA-Tumor	Indeterminate
RB1	CNA-Seq	DNA-Tumor	Amplification Not Detected
RB1	Seq	DNA-Tumor	Indeterminate
RBBP8	Seq	DNA-Tumor	Mutation Not Detected
RBM10	Seq	DNA-Tumor	Mutation Not Detected
RCAN1	Seq	DNA-Tumor	Mutation Not Detected
RECQL4	Seq	DNA-Tumor	Mutation Not Detected
REL	Seq	DNA-Tumor	Mutation Not Detected
RELA	Seq	DNA-Tumor	Indeterminate
RELA	Seq	RNA-Tumor	Fusion Not Detected
RET	CNA-Seq	DNA-Tumor	Amplification Not Detected
RET	Seq	DNA-Tumor	Mutation Not Detected
RET	Seq	RNA-Tumor	Fusion Not Detected
RHEB	Seq	DNA-Tumor	Mutation Not Detected
RHOA	Seq	DNA-Tumor	Mutation Not Detected
RHOH	Seq	DNA-Tumor	Mutation Not Detected
RICTOR	CNA-Seq	DNA-Tumor	Amplification Not Detected
RICTOR	Seq	DNA-Tumor	Mutation Not Detected
RINT1	Seq	DNA-Tumor	Mutation Not Detected
RIT1	Seq	DNA-Tumor	Mutation Not Detected
RMI2	Seq	DNA-Tumor	Mutation Not Detected
RNF43	CNA-Seq	DNA-Tumor	Amplification Not Detected
RNF43	Seq	DNA-Tumor	Mutation Not Detected
ROS1	CNA-Seq	DNA-Tumor	Amplification Not Detected
ROS1	Seq	DNA-Tumor	Mutation Not Detected
ROS1	Seq	RNA-Tumor	Fusion Not Detected
RPA1	Seq	DNA-Tumor	Indeterminate
RPA2	Seq	DNA-Tumor	Mutation Not Detected
RPA3	Seq	DNA-Tumor	Mutation Not Detected
RPA4	Seq	DNA-Tumor	Mutation Not Detected
RPL10	Seq	DNA-Tumor	Mutation Not Detected
RPL22	CNA-Seq	DNA-Tumor	Amplification Not Detected
RPL22	Seq	DNA-Tumor	Mutation Not Detected
RPL5	Seq	DNA-Tumor	Mutation Not Detected
RPN1	CNA-Seq	DNA-Tumor	Amplification Not Detected
RPN1	Seq	DNA-Tumor	Mutation Not Detected
RPTOR	Seq	DNA-Tumor	Mutation Not Detected
RRAS2	Seq	DNA-Tumor	Mutation Not Detected
RSPO1	Seq	DNA-Tumor	Mutation Not Detected
RSPO2	Seq	DNA-Tumor	Mutation Not Detected
RSPO2	Seq	RNA-Tumor	Fusion Not Detected
RSPO3	Seq	DNA-Tumor	Mutation Not Detected
RSPO3	Seq	RNA-Tumor	Fusion Not Detected
RUNX1	CNA-Seq	DNA-Tumor	Amplification Not Detected
RUNX1	Seq	DNA-Tumor	Mutation Not Detected
RUNx1T1	CNA-Seq	DNA-Tumor	Amplification Not Detected
RUNx1T1	Seq	DNA-Tumor	Mutation Not Detected
SBDS	CNA-Seq	DNA-Tumor	Amplification Not Detected
SBDS	Seq	DNA-Tumor	Mutation Not Detected
SDC4	CNA-Seq	DNA-Tumor	Amplification Not Detected
SDC4	Seq	DNA-Tumor	Mutation Not Detected
SDHA	Seq	DNA-Tumor	Mutation Not Detected
SDHAF2	CNA-Seq	DNA-Tumor	Amplification Not Detected
SDHAF2	Seq	DNA-Tumor	Mutation Not Detected
SDHB	CNA-Seq	DNA-Tumor	Amplification Not Detected
SDHB	Seq	DNA-Tumor	Mutation Not Detected
SDHC	CNA-Seq	DNA-Tumor	Amplification Not Detected
SDHC	Seq	DNA-Tumor	Mutation Not Detected
SDHD	CNA-Seq	DNA-Tumor	Amplification Not Detected
SDHD	Seq	DNA-Tumor	Mutation Not Detected
SEM1	Seq	DNA-Tumor	Mutation Not Detected
SERPINB3	Seq	DNA-Tumor	Mutation Not Detected
SET	Seq	DNA-Tumor	Indeterminate
SETBP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
SETBP1	Seq	DNA-Tumor	Mutation Not Detected
SETD2	CNA-Seq	DNA-Tumor	Amplification Not Detected
SETD2	Seq	DNA-Tumor	Mutation Not Detected
SF3B1	CNA-Seq	DNA-Tumor	Amplification Not Detected
SF3B1	Seq	DNA-Tumor	Mutation Not Detected
SFPQ	Seq	DNA-Tumor	Mutation Not Detected
SGK1	Seq	DNA-Tumor	Mutation Not Detected
SH2B3	Seq	DNA-Tumor	Mutation Not Detected
SLC34A2	CNA-Seq	DNA-Tumor	Amplification Not Detected
SLC34A2	Seq	DNA-Tumor	Mutation Not Detected
SLIT2	Seq	DNA-Tumor	Mutation Not Detected
SLX4	Seq	DNA-Tumor	Mutation Not Detected
SMAD2	CNA-Seq	DNA-Tumor	Amplification Not Detected
SMAD2	Seq	DNA-Tumor	Mutation Not Detected
SMAD3	Seq	DNA-Tumor	Mutation Not Detected
SMAD4	CNA-Seq	DNA-Tumor	Amplification Not Detected
SMAD4	Seq	DNA-Tumor	Mutation Not Detected
SMARCA1	Seq	DNA-Tumor	Indeterminate
SMARCA4	Seq	DNA-Tumor	Mutation Not Detected
SMARCB1	CNA-Seq	DNA-Tumor	Amplification Not Detected
SMARCB1	Seq	DNA-Tumor	Mutation Not Detected
SMARCE1	CNA-Seq	DNA-Tumor	Amplification Not Detected
SMARCE1	Seq	DNA-Tumor	Mutation Not Detected
SMC3	Seq	DNA-Tumor	Mutation Not Detected
SMO	CNA-Seq	DNA-Tumor	Amplification Not Detected
SMO	Seq	DNA-Tumor	Mutation Not Detected
SNCAIP	Seq	DNA-Tumor	Indeterminate
SNORA50A	Seq	RNA-Tumor	Unclassified Fusion Detected	SNORA50A:CNOT1 |
SNX29	CNA-Seq	DNA-Tumor	Amplification Not Detected
SNX29	Seq	DNA-Tumor	Mutation Not Detected
SOCS1	Seq	DNA-Tumor	Mutation Not Detected
SOS1	Seq	DNA-Tumor	Mutation Not Detected
SOX10	CNA-Seq	DNA-Tumor	Amplification Not Detected
SOX10	Seq	DNA-Tumor	Mutation Not Detected
SOX2	Seq	DNA-Tumor	Mutation Not Detected
SOX9	Seq	DNA-Tumor	Mutation Not Detected
SPECC1	CNA-Seq	DNA-Tumor	Amplification Not Detected
SPECC1	Seq	DNA-Tumor	Mutation Not Detected
SPEN	CNA-Seq	DNA-Tumor	Amplification Not Detected
SPEN	Seq	DNA-Tumor	Mutation Not Detected
SPOP	Seq	DNA-Tumor	Mutation Not Detected
SPRED1	Seq	DNA-Tumor	Mutation Not Detected
SPTA1	Seq	DNA-Tumor	Mutation Not Detected
SRC	Seq	DNA-Tumor	Mutation Not Detected
SRGAP3	CNA-Seq	DNA-Tumor	Amplification Not Detected
SRGAP3	Seq	DNA-Tumor	Mutation Not Detected
SRSF2	CNA-Seq	DNA-Tumor	Amplification Not Detected
SRSF2	Seq	DNA-Tumor	Mutation Not Detected
SRSF3	CNA-Seq	DNA-Tumor	Amplification Not Detected
SRSF3	Seq	DNA-Tumor	Mutation Not Detected
SS18	Seq	DNA-Tumor	Mutation Not Detected
SSBP1	Seq	DNA-Tumor	Mutation Not Detected
STAG2	Seq	DNA-Tumor	Indeterminate
STAT3	CNA-Seq	DNA-Tumor	Amplification Not Detected
STAT3	Seq	DNA-Tumor	Mutation Not Detected
STAT4	Seq	DNA-Tumor	Mutation Not Detected
STAT5B	CNA-Seq	DNA-Tumor	Amplification Not Detected
STAT5B	Seq	DNA-Tumor	Mutation Not Detected
STAT6	Seq	DNA-Tumor	Mutation Not Detected
STIL	CNA-Seq	DNA-Tumor	Amplification Not Detected
STIL	Seq	DNA-Tumor	Mutation Not Detected
STK11	CNA-Seq	DNA-Tumor	Amplification Not Detected
STK11	Seq	DNA-Tumor	Mutation Not Detected
SUFU	CNA-Seq	DNA-Tumor	Amplification Not Detected
SUFU	Seq	DNA-Tumor	Mutation Not Detected
SUZ12	CNA-Seq	DNA-Tumor	Amplification Not Detected
SUZ12	Seq	DNA-Tumor	Mutation Not Detected
SYK	CNA-Seq	DNA-Tumor	Amplification Not Detected
SYK	Seq	DNA-Tumor	Mutation Not Detected
TAF1	Seq	DNA-Tumor	Mutation Not Detected
TAF15	CNA-Seq	DNA-Tumor	Amplification Not Detected
TAF15	Seq	DNA-Tumor	Mutation Not Detected
TAL1	Seq	DNA-Tumor	Mutation Not Detected
TAL2	Seq	DNA-Tumor	Mutation Not Detected
TBX3	Seq	DNA-Tumor	Mutation Not Detected
TBXT	Seq	DNA-Tumor	Mutation Not Detected
TCEA1	Seq	DNA-Tumor	Mutation Not Detected
TCF3	Seq	DNA-Tumor	Mutation Not Detected
TCF7L2	CNA-Seq	DNA-Tumor	Amplification Not Detected
TCF7L2	Seq	DNA-Tumor	Mutation Not Detected
TEK	Seq	DNA-Tumor	Mutation Not Detected
TERC	Seq	DNA-Tumor	Mutation Not Detected
TERF2IP	Seq	DNA-Tumor	Mutation Not Detected
TERT	Seq	DNA-Tumor	Indeterminate
TERT	Seq	RNA-Tumor	Fusion Not Detected
TET1	CNA-Seq	DNA-Tumor	Amplification Not Detected
TET1	Seq	DNA-Tumor	Mutation Not Detected
TET2	Seq	DNA-Tumor	Mutation Not Detected
TFE3	Seq	DNA-Tumor	Mutation Not Detected
TFE3	Seq	RNA-Tumor	Fusion Not Detected
TFEB	Seq	DNA-Tumor	Mutation Not Detected
TFEB	Seq	RNA-Tumor	Fusion Not Detected
TFG	Seq	DNA-Tumor	Mutation Not Detected
TFRC	CNA-Seq	DNA-Tumor	Amplification Not Detected
TFRC	Seq	DNA-Tumor	Mutation Not Detected
TGFBR1	Seq	DNA-Tumor	Indeterminate
TGFBR2	CNA-Seq	DNA-Tumor	Amplification Not Detected
TGFBR2	Seq	DNA-Tumor	Mutation Not Detected
THADA	Seq	RNA-Tumor	Fusion Not Detected
THRAP3	Seq	DNA-Tumor	Mutation Not Detected
TIPARP	Seq	DNA-Tumor	Mutation Not Detected
TLX3	Seq	DNA-Tumor	Mutation Not Detected
TMB	Seq	DNA-Tumor	Low	4 Mutations/Mb	0
TMEM127	Seq	DNA-Tumor	Mutation Not Detected
TMPRSS2	Seq	RNA-Tumor	Fusion Not Detected
TNF	Seq	DNA-Tumor	Mutation Not Detected
TNFAIP3	CNA-Seq	DNA-Tumor	Amplification Not Detected
TNFAIP3	Seq	DNA-Tumor	Mutation Not Detected
TNFRSF14	CNA-Seq	DNA-Tumor	Amplification Not Detected
TNFRSF14	Seq	DNA-Tumor	Mutation Not Detected
TOP1	CNA-Seq	DNA-Tumor	Amplification Not Detected
TOP1	Seq	DNA-Tumor	Mutation Not Detected
TOP2A	Seq	DNA-Tumor	Mutation Not Detected
TOP3A	Seq	DNA-Tumor	Mutation Not Detected
TOP3B	Seq	DNA-Tumor	Mutation Not Detected
TP53	CNA-Seq	DNA-Tumor	Amplification Not Detected
TP53	Seq	DNA-Tumor	Pathogenic Variant	Exon 7 | p.R248Q	38
TPM3	CNA-Seq	DNA-Tumor	Amplification Not Detected
TPM3	Seq	DNA-Tumor	Mutation Not Detected
TPM4	CNA-Seq	DNA-Tumor	Amplification Not Detected
TPM4	Seq	DNA-Tumor	Mutation Not Detected
TRAF3	Seq	DNA-Tumor	Mutation Not Detected
TRAF7	Seq	DNA-Tumor	Indeterminate
TRIM27	CNA-Seq	DNA-Tumor	Amplification Not Detected
TRIM27	Seq	DNA-Tumor	Mutation Not Detected
TRRAP	CNA-Seq	DNA-Tumor	Amplification Not Detected
TRRAP	Seq	DNA-Tumor	Mutation Not Detected
TSC1	CNA-Seq	DNA-Tumor	Amplification Not Detected
TSC1	Seq	DNA-Tumor	Mutation Not Detected
TSC2	CNA-Seq	DNA-Tumor	Amplification Not Detected
TSC2	Seq	DNA-Tumor	Mutation Not Detected
TSHR	CNA-Seq	DNA-Tumor	Amplification Not Detected
TSHR	Seq	DNA-Tumor	Indeterminate
TSHZ3	Seq	DNA-Tumor	Mutation Not Detected
TYK2	Seq	DNA-Tumor	Mutation Not Detected
TYRO3	Seq	DNA-Tumor	Indeterminate
U2AF1	CNA-Seq	DNA-Tumor	Amplification Not Detected
U2AF1	Seq	DNA-Tumor	Mutation Not Detected
UBE2T	Seq	DNA-Tumor	Mutation Not Detected
UBR5	Seq	DNA-Tumor	Mutation Not Detected
USP6	CNA-Seq	DNA-Tumor	Amplification Not Detected
USP6	Seq	DNA-Tumor	Mutation Not Detected
VEGFA	Seq	DNA-Tumor	Mutation Not Detected
VHL	Seq	DNA-Tumor	Mutation Not Detected
VTI1A	CNA-Seq	DNA-Tumor	Amplification Not Detected
VTI1A	Seq	DNA-Tumor	Mutation Not Detected
WAS	Seq	DNA-Tumor	Mutation Not Detected
WDCP	CNA-Seq	DNA-Tumor	Amplification Not Detected
WDCP	Seq	DNA-Tumor	Mutation Not Detected
WISP3	CNA-Seq	DNA-Tumor	Amplification Not Detected
WISP3	Seq	DNA-Tumor	Mutation Not Detected
WRN	CNA-Seq	DNA-Tumor	Amplification Not Detected
WRN	Seq	DNA-Tumor	Mutation Not Detected
WT1	CNA-Seq	DNA-Tumor	Amplification Not Detected
WT1	Seq	DNA-Tumor	Mutation Not Detected
WWTR1	CNA-Seq	DNA-Tumor	Amplification Not Detected
WWTR1	Seq	DNA-Tumor	Mutation Not Detected
XPA	Seq	DNA-Tumor	Mutation Not Detected
XPC	CNA-Seq	DNA-Tumor	Amplification Not Detected
XPC	Seq	DNA-Tumor	Mutation Not Detected
XPO1	Seq	DNA-Tumor	Mutation Not Detected
XRCC1	Seq	DNA-Tumor	Mutation Not Detected
XRCC2	Seq	DNA-Tumor	Indeterminate
XRCC3	Seq	DNA-Tumor	Mutation Not Detected
YAP1	Seq	DNA-Tumor	Unclassified Variant	Exon 8 | p.G401V	8
YES1	Seq	DNA-Tumor	Mutation Not Detected
YWHAE	CNA-Seq	DNA-Tumor	Amplification Not Detected
YWHAE	Seq	DNA-Tumor	Mutation Not Detected
ZBTB16	Seq	DNA-Tumor	Mutation Not Detected
ZBTB2	Seq	DNA-Tumor	Mutation Not Detected
ZFHX3	Seq	DNA-Tumor	Mutation Not Detected
ZNF217	CNA-Seq	DNA-Tumor	Amplification Not Detected
ZNF217	Seq	DNA-Tumor	Mutation Not Detected
ZNF331	CNA-Seq	DNA-Tumor	Amplification Not Detected
ZNF331	Seq	DNA-Tumor	Mutation Not Detected
ZNF384	CNA-Seq	DNA-Tumor	Amplification Not Detected
ZNF384	Seq	DNA-Tumor	Mutation Not Detected
ZNF521	CNA-Seq	DNA-Tumor	Amplification Not Detected
ZNF521	Seq	DNA-Tumor	Mutation Not Detected
ZNF703	Seq	DNA-Tumor	Mutation Not Detected
ZNRF3	Seq	DNA-Tumor	Mutation Not Detected
ZRSR2	Seq	DNA-Tumor	Indeterminate