| Literature DB >> 35941476 |
Mei Han1, Richard Smith2, Dan A Rock2.
Abstract
Both capillary electrophoresis (CE) and mass spectrometry (MS) technologies are powerful analytical tools that have been used extensively in the characterization of biologics in the biopharmaceutical industry. The direct coupling of CE with MS is an attractive approach, in that the high separation capability of CE and the ultrasensitive detection and accurate identification performance of MS can be combined to provide a powerful system for the analysis of complex analytes. In this chapter, we discuss the detailed procedure of carrying out CE-MS analysis using a nano sheath-flow interface and its applications including intact mass analysis of monoclonal antibodies and fusion proteins, and a biotransformation study of two Fc-FGF21 molecules in a single-dose pharmacokinetic mice study. Optimization processes, including the finetuning of CE conditions and MS parameters, are illustrated in this chapter, with focuses on method robustness and assay reproducibility.Entities:
Keywords: Affinity capture; Affinity purification; Biotransformation; CE-MS; Capillary electrophoresis–mass spectrometry; FGF21; Fusion protein; Intact mass analysis; Monoclonal antibody; Time-of-flight
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Year: 2022 PMID： 35941476 DOI： 10.1007/978-1-0716-2493-7_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745