Manikandan Velayutham1, B Haridevamuthu1, P Snega Priya1, Munuswamy Ramanujam Ganesh2, Annie Juliet3, Jesu Arockiaraj4. 1. Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, 603 203, Kattankulathur, Chennai, Tamil Nadu, India. 2. Department of Chemistry, Interdisciplinary Institute of Indian System of Medicine, SRM Institute of Science and Technology, 603 203, Kattankulathur, Chennai, Tamil Nadu, India. 3. Institute for Cellular and Molecular Biology, The University of Texas at Austin, University Station A4800, 78712, Austin, TX, USA. 4. Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, 603 203, Kattankulathur, Chennai, Tamil Nadu, India. jesuaraj@hotmail.com.
Abstract
BACKGROUND: Most of the bioactive peptides exhibit antioxidant effect and do elicit inhibitory effect on proliferation of cancer cells. This study investigates the in-vitro antioxidant and anti-cancer properties of NV14 peptide, derived from serine O-acetyltransferase (SAT) of spirulina, Arthrospira platensis. METHODS: The anti-cancer effect of the peptide was evaluated using human adenocarcinoma epithelial cells (MCF-7), while the anti-oxidant potential, as in reduction in ROS concentration, has been established using the H2O2-exposed, Madin-Darby canine kidney (MDCK) cells. The outcome of the in vitro analyses has been evaluated by in silico molecular docking analyses. RESULTS: The peptide, dose-dependently, reduced oxidative stress as well as cell proliferation. Besides, based on the binding scores between NV14 peptide and the important proteins associated with apoptosis and antioxidant defense, it is evident that the peptide has antioxidant and anti-cancer effect, in vitro. CONCLUSIONS: Together, this study demonstrates that NV14 has a potent antioxidant and anti-cancer capability; however, further direction needs to be focused on clinical or pharmacodynamics aspects.
BACKGROUND: Most of the bioactive peptides exhibit antioxidant effect and do elicit inhibitory effect on proliferation of cancer cells. This study investigates the in-vitro antioxidant and anti-cancer properties of NV14 peptide, derived from serine O-acetyltransferase (SAT) of spirulina, Arthrospira platensis. METHODS: The anti-cancer effect of the peptide was evaluated using human adenocarcinoma epithelial cells (MCF-7), while the anti-oxidant potential, as in reduction in ROS concentration, has been established using the H2O2-exposed, Madin-Darby canine kidney (MDCK) cells. The outcome of the in vitro analyses has been evaluated by in silico molecular docking analyses. RESULTS: The peptide, dose-dependently, reduced oxidative stress as well as cell proliferation. Besides, based on the binding scores between NV14 peptide and the important proteins associated with apoptosis and antioxidant defense, it is evident that the peptide has antioxidant and anti-cancer effect, in vitro. CONCLUSIONS: Together, this study demonstrates that NV14 has a potent antioxidant and anti-cancer capability; however, further direction needs to be focused on clinical or pharmacodynamics aspects.
Authors: Robin J Marles; Marilyn L Barrett; Joanne Barnes; Mary L Chavez; Paula Gardiner; Richard Ko; Gail B Mahady; Tieraona Low Dog; Nandakumara D Sarma; Gabriel I Giancaspro; Maged Sharaf; James Griffiths Journal: Crit Rev Food Sci Nutr Date: 2011-08 Impact factor: 11.176
Authors: Arthur G Mikaelian; Eric Traboulay; Xiaofei Michael Zhang; Emma Yeritsyan; Peter L Pedersen; Young Hee Ko; Khalid Z Matalka Journal: Drug Des Devel Ther Date: 2020-02-27 Impact factor: 4.162