Kannikar Vongbhavit1,2, Lauren K Salinero3, Karen M Kalanetra3, Chad Masarweh3, Alice Yu3, Diana H Taft3, David A Mills3, Mark A Underwood4. 1. Department of Pediatrics, HRH Princess Maha Chakri Sirindhorn Medical Center, Srinakharinwirot University, Nakornayok, Thailand. 2. Division of Neonatology, Department of Pediatrics, University of California Davis, Sacramento, CA, USA. 3. Department of Food Science and Technology, University of California Davis, Davis, CA, USA. 4. Division of Neonatology, Department of Pediatrics, University of California Davis, Sacramento, CA, USA. munderwood@ucdavis.edu.
Abstract
OBJECTIVE: Feeding tubes harbor microbial contaminants; studies to date have not explored differences between orogastric (OG) and nasogastric (NG) tube biofilms. We sought to extend a previous analysis by comparing bacterial colonization by location (OG v NG) and by evaluating clinical factors that may affect tube bacterial populations. STUDY DESIGN: The pharyngeal segments of 41 infant feeding tubes (14 OG and 27 NG) from 41 infants were analyzed by next generation 16 S rRNA sequencing on the MiSeq platform. RESULTS: At the phylum level, Proteobacteria had the highest relative abundance of both OG and NG tubes. At the genus/species level, nine taxa differed significantly between OG and NG tubes. Alpha and beta diversity analyses showed significant differences between OG and NG tubes with relatively little contribution from clinical factors. CONCLUSION: The route of feeding tube insertion (oral vs nasal) had a greater impact on bacterial colonization than the assessed clinical factors.
OBJECTIVE: Feeding tubes harbor microbial contaminants; studies to date have not explored differences between orogastric (OG) and nasogastric (NG) tube biofilms. We sought to extend a previous analysis by comparing bacterial colonization by location (OG v NG) and by evaluating clinical factors that may affect tube bacterial populations. STUDY DESIGN: The pharyngeal segments of 41 infant feeding tubes (14 OG and 27 NG) from 41 infants were analyzed by next generation 16 S rRNA sequencing on the MiSeq platform. RESULTS: At the phylum level, Proteobacteria had the highest relative abundance of both OG and NG tubes. At the genus/species level, nine taxa differed significantly between OG and NG tubes. Alpha and beta diversity analyses showed significant differences between OG and NG tubes with relatively little contribution from clinical factors. CONCLUSION: The route of feeding tube insertion (oral vs nasal) had a greater impact on bacterial colonization than the assessed clinical factors.
Authors: John R Mehall; Cheryl A Kite; Daniel A Saltzman; Traci Wallett; Richard J Jackson; Samuel D Smith Journal: J Pediatr Surg Date: 2002-08 Impact factor: 2.545
Authors: Edward Hurrell; Eva Kucerova; Michael Loughlin; Juncal Caubilla-Barron; Anthony Hilton; Richard Armstrong; Craig Smith; Judith Grant; Shiu Shoo; Stephen Forsythe Journal: BMC Infect Dis Date: 2009-09-01 Impact factor: 3.090