Literature DB >> 3580481

A theoretical analysis of the effects of sonication on differential absorption flattening in suspensions of membrane sheets.

C L Teeters, J Eccles, B A Wallace.   

Abstract

A theoretical analysis is presented for the flattening of absorption and circular dichroic spectra of suspensions of membrane sheets containing proteins. Equations are presented to describe the dependence of this artifact on the size of the sheets. Values for the flattening coefficients QA and QB are calculated both as a function of absorptivity and sheet size. These studies show that sonication in an inadequate procedure for eliminating flattening in these samples.

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Year:  1987        PMID： 3580481      PMCID： PMC1329925          DOI： 10.1016/S0006-3495(87)83378-7

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  7 in total

1.  The flattening of the absorption spectrum of suspensions, as compared to that of solutions.

Authors:  L N DUYSENS
Journal:  Biochim Biophys Acta       Date:  1956-01

2.  Further characterization of protein secondary structures in purple membrane by circular dichroism and polarized infrared spectroscopies.

Authors:  E Nabedryk; A M Bardin; J Breton
Journal:  Biophys J       Date:  1985-12       Impact factor: 4.033

3.  An experimental method correcting for absorption flattening and scattering in suspensions of absorbing particles: circular dichroism and absorption spectra of hemoglobin in situ in red blood cells.

Authors:  A S Schneider; D Harmatz
Journal:  Biochemistry       Date:  1976-09-21       Impact factor: 3.162

Review 4.  Protein conformation in biomembranes: optical rotation and absorption of membrane suspensions.

Authors:  D W Urry
Journal:  Biochim Biophys Acta       Date:  1972-02-14

5.  Artifacts in the measured optic activity of membrane suspensions.

Authors:  D J Gordon; G Holzwarth
Journal:  Arch Biochem Biophys       Date:  1971-02       Impact factor: 4.013

6.  Differential light scattering and absorption flattening optical effects are minimal in the circular dichroism spectra of small unilamellar vesicles.

Authors:  D Mao; B A Wallace
Journal:  Biochemistry       Date:  1984-06-05       Impact factor: 3.162

7.  Circular dichroism analyses of membrane proteins: an examination of differential light scattering and absorption flattening effects in large membrane vesicles and membrane sheets.

Authors:  B A Wallace; D Mao
Journal:  Anal Biochem       Date:  1984-11-01       Impact factor: 3.365
  7 in total
  4 in total

1.  Analyses of circular dichroism spectra of membrane proteins.

Authors:  B A Wallace; J G Lees; A J W Orry; A Lobley; Robert W Janes
Journal:  Protein Sci       Date:  2003-04       Impact factor: 6.725

2.  Circular-dichroism analyses of membrane proteins: examination of environmental effects on bacteriorhodopsin spectra.

Authors:  N A Swords; B A Wallace
Journal:  Biochem J       Date:  1993-01-01       Impact factor: 3.857

3.  In vitro reassembly of the ribose ATP-binding cassette transporter reveals a distinct set of transport complexes.

Authors:  Matthew C Clifton; Michael J Simon; Satchal K Erramilli; Huide Zhang; Jelena Zaitseva; Mark A Hermodson; Cynthia V Stauffacher
Journal:  J Biol Chem       Date:  2014-12-22       Impact factor: 5.157

4.  Hydrophobic Mismatch Drives the Interaction of E5 with the Transmembrane Segment of PDGF Receptor.

Authors:  Dirk Windisch; Colin Ziegler; Stephan L Grage; Jochen Bürck; Marcel Zeitler; Peter L Gor'kov; Anne S Ulrich
Journal:  Biophys J       Date:  2015-08-18       Impact factor: 4.033
  4 in total

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