Literature DB >> 19431599

Further characterization of protein secondary structures in purple membrane by circular dichroism and polarized infrared spectroscopies.

E Nabedryk, A M Bardin, J Breton.   

Abstract

The conformation and the orientation of the protein secondary structures in purple membrane was analyzed by infrared absorption and linear dichroism of oriented membranes as well as by UV circular dichroism of bacteriorhodopsin in intact purple membrane and in lipid vesicles. A large amount (74 +/- 5%) of transmembrane alpha-helices is detected with no significant contribution of beta-sheet strands running perpendicular to the membrane plane. Thus, these data do not support the recent structural model proposed by Jap et al. (Biophys. J. 1983, 43:81-89).

Entities:  

Year:  1985        PMID： 19431599      PMCID： PMC1329418          DOI： 10.1016/S0006-3495(85)83848-0

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  20 in total

1.  Vibrational analysis of peptides, polypeptides, and proteins: Characteristic amide bands of beta-turns.

Authors:  J Bandekar; S Krimm
Journal:  Proc Natl Acad Sci U S A       Date:  1979-02       Impact factor: 11.205

2.  The structure of the purple membrane from Halobacterium hallobium: analysis of the X-ray diffraction pattern.

Authors:  R Henderson
Journal:  J Mol Biol       Date:  1975-04-05       Impact factor: 5.469

3.  Effects of light adaptation on the purple membrane structure of Halobacterium halobium.

Authors:  B Becher; J Y Cassim
Journal:  Biophys J       Date:  1976-10       Impact factor: 4.033

4.  Polarized infrared spectroscopy of oriented purple membrane.

Authors:  K J Rothschild; N A Clark
Journal:  Biophys J       Date:  1979-03       Impact factor: 4.033

5.  Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.

Authors:  Y H Chen; J T Yang; K H Chau
Journal:  Biochemistry       Date:  1974-07-30       Impact factor: 3.162

6.  Circular dichroism of biological membranes: purple membrane of Halobacterium halobium.

Authors:  M M Long; D W Urry; W Stoeckenius
Journal:  Biochem Biophys Res Commun       Date:  1977-04-11       Impact factor: 3.575

7.  Folding of the mitochondrial proton adenosinetriphosphatase proteolipid channel in phospholipid vesicles.

Authors:  D Mao; E Wachter; B A Wallace
Journal:  Biochemistry       Date:  1982-09-28       Impact factor: 3.162

8.  Differential light scattering and absorption flattening optical effects are minimal in the circular dichroism spectra of small unilamellar vesicles.

Authors:  D Mao; B A Wallace
Journal:  Biochemistry       Date:  1984-06-05       Impact factor: 3.162

9.  The C-terminus of bacteriorhodopsin is a random coil.

Authors:  B A Wallace; N Kohl
Journal:  Biochim Biophys Acta       Date:  1984-10-17

10.  Beta-turns in proteins.

Authors:  P Y Chou; G D Fasman
Journal:  J Mol Biol       Date:  1977-09-15       Impact factor: 5.469
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  11 in total

1.  Effect of membrane potential on the conformation of bacteriorhodopsin reconstituted in lipid vesicles.

Authors:  V Brumfeld; I R Miller
Journal:  Biophys J       Date:  1988-10       Impact factor: 4.033

2.  Evidence for unbenignant nature of glucose as a replacement for water in purple membranes.

Authors:  N J Gibson; J Y Cassim
Journal:  Biophys J       Date:  1993-05       Impact factor: 4.033

3.  The orientation of beta-sheets in porin. A polarized Fourier transform infrared spectroscopic investigation.

Authors:  E Nabedryk; R M Garavito; J Breton
Journal:  Biophys J       Date:  1988-05       Impact factor: 4.033

4.  Evaluation of methods for the prediction of membrane protein secondary structures.

Authors:  B A Wallace; M Cascio; D L Mielke
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

5.  A theoretical analysis of the effects of sonication on differential absorption flattening in suspensions of membrane sheets.

Authors:  C L Teeters; J Eccles; B A Wallace
Journal:  Biophys J       Date:  1987-04       Impact factor: 4.033

6.  Infrared dichroism of amide I and amide II modes of alpha I- and alpha II-helix segments in membrane proteins.

Authors:  W C Reisdorf; S Krimm
Journal:  Biophys J       Date:  1995-07       Impact factor: 4.033

7.  Fourier transform infrared spectroscopy and site-directed isotope labeling as a probe of local secondary structure in the transmembrane domain of phospholamban.

Authors:  C F Ludlam; I T Arkin; X M Liu; M S Rothman; P Rath; S Aimoto; S O Smith; D M Engelman; K J Rothschild
Journal:  Biophys J       Date:  1996-04       Impact factor: 4.033

8.  Polarized Fourier transform infrared spectroscopy of bacteriorhodopsin. Transmembrane alpha helices are resistant to hydrogen/deuterium exchange.

Authors:  T N Earnest; J Herzfeld; K J Rothschild
Journal:  Biophys J       Date:  1990-12       Impact factor: 4.033

9.  Nature of forces stabilizing the transmembrane protein bacteriorhodopsin in purple membrane.

Authors:  N J Gibson; J Y Cassim
Journal:  Biophys J       Date:  1989-10       Impact factor: 4.033

10.  Dehydration-induced molecular structural changes of purple membrane of halobacterium halobium.

Authors:  J E Draheim; N J Gibson; J Y Cassim
Journal:  Biophys J       Date:  1988-11       Impact factor: 4.033
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