Literature DB >> 35796964

CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells.

Akihiko Sakashita1, Masaru Ariura2, Satoshi H Namekawa3.   

Abstract

Mounting evidence has established that subsets of transposable elements (TEs) function as gene regulatory elements in a cell type- and species-specific manner. Here we describe an in vitro system to ectopically activate TEs using CRISPR-mediated activation (CRISPRa) for functional studies in mouse embryonic stem cells (ESCs). We established a stable mouse CRISPRa ESC line, in which expression of guide RNA enables the activation of TE-derived enhancers and the expression of their adjacent genes. We show an example of ectopic activation of TE-derived enhancers that function in male meiosis, as well as the expression of adjacent germline genes in ESCs. This system can also be applied to functional studies of TEs that are not active in ESCs.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  CRISPR activation; Embryonic stem cells; Endogenous retroviruses; Germ cells; Meiosis; Spermatogenesis; Transposable elements

Mesh：

Substances：

Year:  2022        PMID： 35796964     DOI： 10.1007/978-1-0716-2380-0_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

Review 1.  PIWI-Interacting RNA: Its Biogenesis and Functions.

Authors:  Yuka W Iwasaki; Mikiko C Siomi; Haruhiko Siomi
Journal:  Annu Rev Biochem       Date:  2015-03-05       Impact factor: 23.643

Review 2.  Transposable elements: an abundant and natural source of regulatory sequences for host genes.

Authors:  Rita Rebollo; Mark T Romanish; Dixie L Mager
Journal:  Annu Rev Genet       Date:  2012-08-16       Impact factor: 16.830

Review 3.  Regulatory activities of transposable elements: from conflicts to benefits.

Authors:  Edward B Chuong; Nels C Elde; Cédric Feschotte
Journal:  Nat Rev Genet       Date:  2016-11-21       Impact factor: 53.242

4.  Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.

Authors:  E Li; T H Bestor; R Jaenisch
Journal:  Cell       Date:  1992-06-12       Impact factor: 41.582

Review 5.  Long Terminal Repeats: From Parasitic Elements to Building Blocks of the Transcriptional Regulatory Repertoire.

Authors:  Peter J Thompson; Todd S Macfarlan; Matthew C Lorincz
Journal:  Mol Cell       Date:  2016-06-02       Impact factor: 17.970

Review 6.  Transposable elements in human genetic disease.

Authors:  Lindsay M Payer; Kathleen H Burns
Journal:  Nat Rev Genet       Date:  2019-09-12       Impact factor: 53.242

7.  Highly efficient Cas9-mediated transcriptional programming.

Authors:  Alejandro Chavez; Jonathan Scheiman; Suhani Vora; Benjamin W Pruitt; Marcelle Tuttle; Eswar P R Iyer; Shuailiang Lin; Samira Kiani; Christopher D Guzman; Daniel J Wiegand; Dmitry Ter-Ovanesyan; Jonathan L Braff; Noah Davidsohn; Benjamin E Housden; Norbert Perrimon; Ron Weiss; John Aach; James J Collins; George M Church
Journal:  Nat Methods       Date:  2015-03-02       Impact factor: 28.547

8.  Endogenous retroviruses drive species-specific germline transcriptomes in mammals.

Authors:  Akihiko Sakashita; So Maezawa; Kazuki Takahashi; Kris G Alavattam; Masashi Yukawa; Yueh-Chiang Hu; Shohei Kojima; Nicholas F Parrish; Artem Barski; Mihaela Pavlicev; Satoshi H Namekawa
Journal:  Nat Struct Mol Biol       Date:  2020-09-07       Impact factor: 15.369

Review 9.  Ten things you should know about transposable elements.

Authors:  Guillaume Bourque; Kathleen H Burns; Mary Gehring; Vera Gorbunova; Andrei Seluanov; Molly Hammell; Michaël Imbeault; Zsuzsanna Izsvák; Henry L Levin; Todd S Macfarlan; Dixie L Mager; Cédric Feschotte
Journal:  Genome Biol       Date:  2018-11-19       Impact factor: 13.583

10.  Co-opted transposons help perpetuate conserved higher-order chromosomal structures.

Authors:  Mayank Nk Choudhary; Ryan Z Friedman; Julia T Wang; Hyo Sik Jang; Xiaoyu Zhuo; Ting Wang
Journal:  Genome Biol       Date:  2020-01-24       Impact factor: 13.583
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