Literature DB >> 35796957

Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA Sequencing.

Makoto Kashima1, Atsumi Miyata2, Norito Shibata3.   

Abstract

The freshwater planarian Dugesia japonica is a good in vivo model for studying the function of piwi genes in adult pluripotent stem cell (aPSC) due to their abundant aPSCs. Generally, PIWI family proteins encoded by piwi genes bind to small noncoding RNAs called piRNAs (PIWI-interacting piRNAs). The analysis of PIWI-piRNA complexes in the planarian is useful for revealing the functions of piwi genes in the aPSC system. In this chapter, we present an immunoprecipitation protocol for PIWI-piRNA complexes from whole planarians.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Immunoprecipitation; Neoblast; PIWI; Planarian; RNA-binding protein; Stem cell; piRNA

Mesh：

Substances：

Year:  2022        PMID： 35796957     DOI： 10.1007/978-1-0716-2380-0_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

Review 1.  Molecular and cellular aspects of planarian regeneration.

Authors:  K Agata; K Watanabe
Journal:  Semin Cell Dev Biol       Date:  1999-08       Impact factor: 7.727

2.  Distribution of the stem cells (neoblasts) in the planarian Dugesia japonica.

Authors:  Hidefumi Orii; Takashige Sakurai; Kenji Watanabe
Journal:  Dev Genes Evol       Date:  2005-01-19       Impact factor: 0.900

Review 3.  Two different evolutionary origins of stem cell systems and their molecular basis.

Authors:  Kiyokazu Agata; Elizabeth Nakajima; Noriko Funayama; Norito Shibata; Yumi Saito; Yoshihiko Umesono
Journal:  Semin Cell Dev Biol       Date:  2006-05-27       Impact factor: 7.727

4.  DjCBC-1, a conserved DEAD box RNA helicase of the RCK/p54/Me31B family, is a component of RNA-protein complexes in planarian stem cells and neurons.

Authors:  Maki Yoshida-Kashikawa; Norito Shibata; Katsuaki Takechi; Kiyokazu Agata
Journal:  Dev Dyn       Date:  2007-12       Impact factor: 3.780

Review 5.  PIWI-interacting small RNAs: the vanguard of genome defence.

Authors:  Mikiko C Siomi; Kaoru Sato; Dubravka Pezic; Alexei A Aravin
Journal:  Nat Rev Mol Cell Biol       Date:  2011-04       Impact factor: 94.444

Review 6.  PIWI-Interacting RNA: Its Biogenesis and Functions.

Authors:  Yuka W Iwasaki; Mikiko C Siomi; Haruhiko Siomi
Journal:  Annu Rev Biochem       Date:  2015-03-05       Impact factor: 23.643

Review 7.  The cellular and molecular bases of the sponge stem cell systems underlying reproduction, homeostasis and regeneration.

Authors:  Noriko Funayama
Journal:  Int J Dev Biol       Date:  2018       Impact factor: 2.203

Review 8.  Rethinking differentiation: stem cells, regeneration, and plasticity.

Authors:  Alejandro Sánchez Alvarado; Shinya Yamanaka
Journal:  Cell       Date:  2014-03-27       Impact factor: 41.582

9.  Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian.

Authors:  Norito Shibata; Makoto Kashima; Taisuke Ishiko; Osamu Nishimura; Labib Rouhana; Kazuyo Misaki; Shigenobu Yonemura; Kuniaki Saito; Haruhiko Siomi; Mikiko C Siomi; Kiyokazu Agata
Journal:  Dev Cell       Date:  2016-05-09       Impact factor: 12.270

Review 10.  Piwi and potency: PIWI proteins in animal stem cells and regeneration.

Authors:  Josien C van Wolfswinkel
Journal:  Integr Comp Biol       Date:  2014-06-19       Impact factor: 3.326
  10 in total

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