A Koro1, I Elezaj2, S Hadžiabdić3, A Alić4, E Rešidbegović5. 1. Ph.D. Student in Veterinary Medicine and Public Health, Department of Poultry Breeding, Production & Health Care, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. 2. MSc, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. 3. German Federal Institute for Risk Assessment, Unit Food Hygiene and Technologies, Supply Chains, Food Defense, Department of Biological Safety, Berlin, Germany. 4. Department of Pathology, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. 5. Department of Poultry Breeding, Production & Health Care, Veterinary Faculty, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Abstract
Background: Infected poultry is one of the most important reservoirs of Salmonella. Aims: The investigation presented here was conducted to examine the occurrence of Salmonella in fecal samples among selected flocks of backyard poultry in Bosnia and Herzegovina (B&H). Methods: Isolation and identification of Salmonella was performed in accordance with BAS EN ISO 6579/AMD 1:2007. When genus Salmonella was confirmed, the determination of the antigenic formula of Salmonella isolates was performed in accordance with BAS CEN ISO/TR 6579-3:2015. After that, Salmonella serotypes were subjected to antibiotic susceptibility testing using EUVSEC sensititre microtiter plates impregnated with different concentrations of antibiotics. At the end, real-time PCR was used to detect extended spectrum β-lactamases (ESBL) and carbapeneamase encoding genes (bla TEM, bla SHV, bla CTX-M, bla CMY, bla KPC, bla NDM, bla OXA-48, bla VIM and bla GES). Results: Salmonella spp. was detected in pooled feces from four backyards, housed by chickens only. Three isolates were confirmed by slide agglutination as serotype Enteritidis and one as serotype Typhimurium. Antibiotic susceptibility testing by microdilution did not reveal phenotypical resistance among these four isolates. Real-time PCR used to detect ESBL and carbapeneamase encoding genes revealed the bla TEM gene in one S. Enteritidis isolate. Conclusion: Data presented in this study provide further evidence on the circulation of different Salmonella serotypes in backyard poultry in B&H. These findings emphasize the potential role of backyard poultry in the epidemiology of salmonellosis and the risks it poses for keepers, consumers, and general public health.
Background: Infected poultry is one of the most important reservoirs of Salmonella. Aims: The investigation presented here was conducted to examine the occurrence of Salmonella in fecal samples among selected flocks of backyard poultry in Bosnia and Herzegovina (B&H). Methods: Isolation and identification of Salmonella was performed in accordance with BAS EN ISO 6579/AMD 1:2007. When genus Salmonella was confirmed, the determination of the antigenic formula of Salmonella isolates was performed in accordance with BAS CEN ISO/TR 6579-3:2015. After that, Salmonella serotypes were subjected to antibiotic susceptibility testing using EUVSEC sensititre microtiter plates impregnated with different concentrations of antibiotics. At the end, real-time PCR was used to detect extended spectrum β-lactamases (ESBL) and carbapeneamase encoding genes (bla TEM, bla SHV, bla CTX-M, bla CMY, bla KPC, bla NDM, bla OXA-48, bla VIM and bla GES). Results: Salmonella spp. was detected in pooled feces from four backyards, housed by chickens only. Three isolates were confirmed by slide agglutination as serotype Enteritidis and one as serotype Typhimurium. Antibiotic susceptibility testing by microdilution did not reveal phenotypical resistance among these four isolates. Real-time PCR used to detect ESBL and carbapeneamase encoding genes revealed the bla TEM gene in one S. Enteritidis isolate. Conclusion: Data presented in this study provide further evidence on the circulation of different Salmonella serotypes in backyard poultry in B&H. These findings emphasize the potential role of backyard poultry in the epidemiology of salmonellosis and the risks it poses for keepers, consumers, and general public health.
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