Literature DB >> 35773581

Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae.

Landon Haslem1, Marina Brown1, Xin A Zhang2,3, Jennifer M Hays1, Franklin A Hays4,5.   

Abstract

Membrane protein (MP) functional and structural characterization requires large quantities of high-purity protein for downstream studies. Barriers to MP characterization include ample overexpression, solubilization, and purification of target proteins while maintaining native activity and structure. These barriers can be overcome by utilizing an efficient purification protocol in a high-yield eukaryotic expression system such as Saccharomyces cerevisiae. S. cerevisiae offers improved protein folding and posttranslational modifications compared to prokaryotic expression systems. This chapter contains practices used to overcome barriers of solubilization and purification using S. cerevisiae that are broadly applicable to diverse membrane associated, and membrane integrated, protein targets.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Chromatography; Membrane protein; Protein production; Purification; Saccharomyces cerevisiae

Mesh：

Substances：

Year:  2022        PMID： 35773581      PMCID： PMC9400948          DOI： 10.1007/978-1-0716-2368-8_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  57 in total

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2.  Reconstitution of membrane proteins into liposomes.

Authors:  Jean-Louis Rigaud; Daniel Lévy
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3.  NMR structure determination of a membrane protein with two transmembrane helices in micelles: MerF of the bacterial mercury detoxification system.

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4.  Designer short peptide surfactants stabilize G protein-coupled receptor bovine rhodopsin.

Authors:  Xiaojun Zhao; Yusuke Nagai; Philip J Reeves; Patrick Kiley; H Gobind Khorana; Shuguang Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2006-11-10       Impact factor: 11.205

Review 5.  Ion-channel reconstitution.

Authors:  Francisco J Morera; Guillermo Vargas; Carlos González; Eduardo Rosenmann; Ramon Latorre
Journal:  Methods Mol Biol       Date:  2007

Review 6.  Lipopeptide detergents for membrane protein studies.

Authors:  Gilbert G Privé
Journal:  Curr Opin Struct Biol       Date:  2009-08-12       Impact factor: 6.809

Review 7.  Crystallization of membrane proteins.

Authors:  C Ostermeier; H Michel
Journal:  Curr Opin Struct Biol       Date:  1997-10       Impact factor: 6.809

Review 8.  Radioligand binding methods: practical guide and tips.

Authors:  D B Bylund; M L Toews
Journal:  Am J Physiol       Date:  1993-11

9.  Purification and Bicelle Crystallization for Structure Determination of the E. coli Outer Membrane Protein TamA.

Authors:  Fabian Gruss; Sebastian Hiller; Timm Maier
Journal:  Methods Mol Biol       Date:  2015

10.  Highly Branched Pentasaccharide-Bearing Amphiphiles for Membrane Protein Studies.

Authors:  Muhammad Ehsan; Yang Du; Nicola J Scull; Elena Tikhonova; Jeffrey Tarrasch; Jonas S Mortensen; Claus J Loland; Georgios Skiniotis; Lan Guan; Bernadette Byrne; Brian K Kobilka; Pil Seok Chae
Journal:  J Am Chem Soc       Date:  2016-03-11       Impact factor: 15.419
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