Accuracy of attenuation imaging in the assessment of pediatric hepatic steatosis: correlation with the controlled attenuation parameter.

PURPOSE: This study evaluated the accuracy of attenuation imaging (ATI) for the assessment of hepatic steatosis in pediatric patients, in comparison with the FibroScan vibration-controlled transient elastography controlled attenuation parameter (CAP).
METHODS: Consecutive pediatric patients referred for evaluation of obesity who underwent both ATI and FibroScan between February 2020 and September 2021 were included. The correlation between attenuation coefficient (AC) and CAP values was assessed using the Spearman test. The AC cutoff value for discriminating hepatic steatosis corresponding to a CAP value of 241 dB/m was calculated. Multivariable linear regression analysis was performed to estimate the strength of the association between AC and CAP. The diagnostic accuracy of AC cutoffs was estimated using the imperfect gold-standard methodology based on a two-level Bayesian latent class model.
RESULTS: Seventy patients (median age, 12.5 years; interquartile range, 11.0 to 14.0 years; male:female, 58:12) were included. AC and CAP showed a moderate-to-good correlation (ρ =0.646, P<0.001). Multivariable regression analysis affirmed the significant association between AC and CAP (P<0.001). The correlation was not evident in patients with a body mass index ≥30 kg/m2 (ρ=-0.202, P=0.551). Linear regression revealed that an AC cutoff of 0.66 dB/cm/MHz corresponded to a CAP of 241 dB/m (sensitivity, 0.93; 95% confidence interval [CI], 0.85 to 0.98 and specificity, 0.87; 95% CI, 0.56 to 1.00).
CONCLUSION: ATI showed an acceptable correlation with CAP values in a pediatric population, especially in patients with a body mass index <30 kg/m2. An AC cutoff of 0.66 dB/cm/MHz, corresponding to a CAP of 241 dB/m, can accurately diagnose hepatic steatosis.

Introduction

Nonalcoholic fatty liver disease (NAFLD) is common in pediatric populations, and fatty liver is the most common liver disease in children and adolescents in the United States, being present in 13% of subjects in autopsy studies [1]. It is more common in Asians, boys, obese patients, and patients with prediabetes or diabetes [1-3]. As children with NAFLD have a higher risk of end-stage liver disease [4], cardiovascular disease [5], and hepatocellular carcinoma [6,7], it is obvious that early diagnosis and treatment of pediatric NAFLD are important issues.

Currently, liver biopsy is the most reliable reference standard for the diagnosis of fatty liver, and beyond its role in diagnostic confirmation, it also provides useful information on the degree of liver damage, architectural change, and fibrosis [3]. However, liver biopsy is not practical for screening purposes because of its invasive nature. Various noninvasive imaging modalities, including ultrasonography (US), computed tomography (CT), and magnetic resonance imaging (MRI), have been used to provide alternative methods for the diagnosis of fatty liver [8]. Among them, the FibroScan (Echosens, Paris, France) vibration-controlled transient elastography controlled attenuation parameter (CAP), which enables quantification of hepatic steatosis by measuring the ultrasonic attenuation of the echo wave [9], is widely used because of its noninvasive and reproducible characteristics. The diagnostic accuracy of CAP is well-validated in adults [10] and also relatively wellvalidated in pediatric populations [11,12]. However, it has several disadvantages: (1) it requires a separate FibroScan machine in addition to a general US scanner; (2) CAP is measured in a blind fashion and inevitably includes large vessels, focal hepatic lesions, and ascites; and (3) CAP is affected by abdominal wall thickness [13].

In recent studies, attenuation imaging (ATI), a two-dimensional US-based fat-quantification technique that measures the attenuation coefficient (AC) within a region of interest (ROI) placed on a grayscale US image, has shown acceptable accuracy in the detection of fatty liver in adult populations [14-16]. As ATI enables hepatic fat quantification during a single-sitting US examination, it has a potential role in pediatric patients. However, its diagnostic accuracy has not been clearly validated in a pediatric population. Therefore, the aim of this study was to evaluate the accuracy of ATI in the assessment of hepatic steatosis in a pediatric population by evaluating its correlation with CAP.

Materials and Methods

Compliance with Ethical Standards

This retrospective observational study was conducted at a single tertiary hospital and was approved by the Institutional Review Board (2021-1663). The requirement for informed consent was waived. This retrospective study was conducted in accordance with Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guidelines [17].

Patient Selection and Clinical Data Collection

Consecutive pediatric patients (age ≤18 years) who were referred to the authors’ institution for the evaluation of fatty liver, and who underwent both ATI and FibroScan between February 2020 and September 2021, were included. At the authors’ institution, the diagnostic work-up for NAFLD (including FibroScan and US) was generally indicated in patients with abnormal alanine aminotransferase (ALT) levels (e.g., persistently [>3 months] elevated ALT higher than cutoff value of 52 IU/L for boys and 44 IU/L for girls, or ALT higher than 80 IU/L) with/without body mass index (BMI) higher than the 85th percentile. This work-up was also indicated for patients who showed sudden weight gain regardless of BMI, or incidentally detected imaging findings suspicious of fatty liver (e.g., diffuse increase in echogenicity on US; diffuse decrease in attenuation on CT). ATI was performed in addition to grayscale US if the liver showed high echogenicity resulting in a suspicion of fatty liver. Patients who met any of the following criteria were excluded: (1) an interval between ATI and FibroScan of >7 days; (2) liver transplantation; and (3) confirmation of disease other than fatty liver that might affect the liver (e.g., biliary atresia, metabolic disease, or cardiac disease). None of the patients included in this study were previously reported.

Clinical data including sex, age at examination, BMI, and levels of aspartate transaminase (AST), ALT, gamma-glutamyl transferase (r-GT), fasting glucose, triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were collected.

Controlled Attenuation Parameter

The CAP, which is based on the ultrasonic properties of the backpropagated radiofrequency signals acquired by the FibroScan system [18], was measured by one of three trained radiologists (P.H.K., H.M.Y., and Y.A.C.; with 5, 12, and 27 years of experience in radiology, respectively). The radiologists were blinded to the clinical data except for age and sex. All measurements were performed using the M probe (3.5 MHz) of a FibroScan-502 system. Patients were asked to fast for at least 3 hours before the examination. The probe was placed in the intercostal space, and CAP was measured in the right liver. During the examinations, patients were instructed to lie in the supine position with their right arm raised. The procedure was deemed to be valid only when measurements were obtained at least 10 times.

Attenuation Imaging

The ATI-based AC was routinely measured in patients suspected to have NAFLD by one of three trained radiologists using an Aplio i800 US machine (Canon Medical Systems, Otawara, Japan) using an i8CX1 transducer. It is well known that the US beam is more attenuated by intracellular fat vacuoles than by normal liver tissue, resulting in an increase of the liver parenchymal echogenicity in fatty liver. In contrast to CAP, which is a one-dimensional attenuation based quantification technique from transient elastography, ATI is a two-dimensional attenuation technique based on B-mode US images. It enables quantification of the degree of loss of US energy passing through the liver by calculating the AC. For an accurate measurement of the AC, the systemic influences from the beam profile and internal gain control are automatically removed. The AC then can be calculated as the slope of the line profile [19].

Before the US examination, patients were asked to fast for at least 3 hours and to lie in the supine position with their right arm raised. Grayscale US was initially performed to find the optimal location for observing the liver parenchyma, and then the ATI mode was activated. Patients were asked to hold their breath during the examination, if possible. The sampling was set as wide as possible, and the ROI was placed at least 2 cm below the skin to avoid reverberation artifacts. Of note, structures other than liver parenchyma, such as vessels, were automatically excluded from the ATI measurements on the basis of their intensity variance. When the ROI was placed in an appropriate position, the AC value and an R2 value indicating the reliability of the result were displayed. The ATI measurement was deemed to be appropriate when R2 was 0.80 or more, and the measurement was deemed to be valid only when appropriate measurements were obtained at least five times [19].

Outcomes and Statistical Analysis

The Spearman correlation test was used to estimate the correlation between AC and CAP, using the median of five appropriately measured AC values. In addition, the strength of the association between AC and CAP was evaluated using multivariable linear regression analysis. In detail, univariable linear regression analysis was first performed to explore potential factors associated with CAP. The covariates considered in this regression analysis, which included AC, were as follows: age, sex, BMI, obesity (normal, BMI <85th percentile; overweight, BMI 85th-94th percentile; obesity, BMI 95th-98th percentile; severe obesity, BMI ≥99th percentile; according to 2019 Korean Society of Pediatric Gastroenterology Hepatology and Nutrition clinical practice guideline [20]) and levels of ALT, AST, r-GT, fasting glucose, triglycerides, LDL, and HDL.

Multivariable linear regression analysis was then performed to calculate the adjusted regression coefficient and P-value of AC, including the covariates that showed significant correlations with CAP in the univariable analysis (P<0.05) and ALT, which is a well-known risk factor for fatty liver [21].

A subgroup analysis was also performed to estimate the degree of correlation between CAP and AC within specific subgroups. In this analysis, the study population was grouped not only according to the covariates showing significant correlations with CAP in the linear regression analysis (i.e., sex and obesity), but also according to BMI (with a cutoff value of 30 kg/m2) and ALT. The BMI cutoff of 30 kg/m2 was used for the subgroup analysis because a previous study found that pediatric patients with a BMI ≥30 kg/m2 showed a poor correlation between CAP and MRI proton density fat fraction (PDFF) [12]. Patients were dichotomized using an ALT cutoff value of 52 IU/L for boys and 44 IU/L for girls, which are the suggested cutoff values for discriminating fatty liver [21].

A linear regression model was also used to calculate an AC cutoff value for the diagnosis of fatty liver by determining the AC value corresponding to a CAP value of 241 dB/m, which is the suggested cutoff value for discriminating fatty liver in the Korean pediatric population [12]. In this cited study, the reference standard was MRI-PDFF, with the PDFF cutoff values being 6% for histologic steatosis grade 1 (S1), 17.5% for grade 2 (S2), and 23.3% for grade 3 (S3) [22]. Of note, the histologic steatosis grade is determined according to the proportion of hepatocytes containing fat macrovesicles: S0 for <5%, S1 for 5%-33%, S2 for 34%-66%, and S3 for >66% [23]. Furthermore, AC cutoffs corresponding to 299 dB/m (the suggested CAP cutoff value for discriminating S0-1 from S2-3 based on MRI-PDFF as the reference standard) and 303 dB/m (the suggested CAP cutoff for discriminating S0-2 from S3 based on MRI-PDFF as the reference standard) were also calculated [12].

The diagnostic accuracies of CAP, AC, obesity (BMI 95th-98th percentile) or severe obesity (BMI ≥99th percentile), and ALT cut-offs were estimated using the imperfect gold-standard methodology based on a two-level Bayesian latent class model [24], as previously reported [11,25]. Briefly, the latent class model analysis combines different index tests, all of which are influenced by the unknown true status (disease-positive or disease-negative), which is treated as the latent variable. In this Bayesian approach, disease prevalence is incorporated into the model, and unknown parameters are treated as random variables with a probability distribution, rather than as fixed unknown quantities. The diagnostic accuracies of ATI for discrimination of S0-1 from S2-3 and S0-2 from S3 were not calculated because the cutoff values for BMI and ALT are not clearly established.

As a rule of thumb, the Spearman correlation coefficient is interpreted as follows: 0-0.25, little or no correlation; 0.25-0.50, a fair degree of correlation; 0.50-0.75, moderate-to-good correlation; and 0.75-1.00, very good to excellent correlation [26]. The two-level Bayesian latent class model used to estimate diagnostic accuracy was constructed using a web-based tool (http://mice.tropmedres.ac/home.aspx), whereas the other statistical analyses were conducted using R version 3.1.2 (R Foundation for Statistical Computing, Vienna, Austria). A two-sided P-value of <0.05 was considered statistically significant.

Results

Patients

During the study period, 91 pediatric patients underwent abdominal US for the evaluation of fatty liver. Of these, 20 patients did not undergo ATI since the Aplio i800 US machine providing ATI function was not available at the time of the US exam (instead, the other US machine, which did not provide ATI, was used for grayscale US). Thus, 71 patients underwent both FibroScan and ATI, although in one patient the interval between FibroScan and ATI was longer than 7 days. Consequently, a total of 70 patients (median age, 12.5 years; interquartile range, 11.0 to 14.0 years; male:female, 58:12) were included in the analysis. There was no case of technical failure with either FibroScan or ATI. The interquartile range and median values of AC did not show statistically significant differences depending on BMI (mean±standard deviation: <25 kg/m2, 0.08±0.04; 25-30 kg/m2, 0.06±0.04; ≥30 kg/m2, 0.06±0.02 dB/cm/MHz; P=0.280) or BMI percentile (mean±standard deviation: normal, 0.08±0.04; overweight, 0.07±0.04; obesity, 0.06±0.02; severe obesity, 0.06±0.04 dB/cm/MHz; P=0.371). The detailed demographics and clinical characteristics of the included patients are summarized in Table 1.

Table 1.

Demographics and clinical characteristics of the 70 included patients

Characteristic	Value
Age (year)	12.5 (11.0–14.0)
Sex
Male	58 (82.9)
Female	12 (17.1)
BMI (kg/m2)	26.4±3.8
<25	22 (31.4)
25–30	37 (52.9)
≥30	11 (15.7)
BMI percentile
Normal (<85th percentile)	10 (14.3)
Overweight (85th–94th percentile)	14 (20.0)
Obesity (95th–98th percentile)	6 (8.6)
Severe obesity (≥99th percentile)	40 (57.1)
ALT (IU/L)	106.3±84.9
Normal	22 (31.4)
Abnormal[a)]	48 (68.6)
AST (IU/L)	57.2±36.8
r-GT (IU/L)	49.7±30.6
Fasting glucose (mg/dL)	97.2±9.8
Triglycerides (mg/dL)	133.1±60.1
LDL (mg/dL)	124.3±26.2
HDL (mg/dL)	46.1±9.2
CAP (dB/m)	295.8±43.3
<241	9 (12.9)
≥241	61 (87.1)

Values are presented as median (IQR), number (%), or mean±SD.

BMI, body mass index; ALT, alanine transaminase; AST, aspartate transaminase; r-GT, gamma-glutamyl transferase; LDL, low-density lipoprotein; HDL, high-density lipoprotein; CAP, controlled attenuation parameter; IQR, interquartile range; SD, standard deviation.

Abnormal ALT was defined as elevated ALT (≥52 IU/L for boys, ≥44 IU/L for girls) more than twice the upper limit of normal (26 IU/L for boys, 22 IU/L for girls).

Correlation between CAP and ATI

The Spearman correlation test demonstrated a good correlation between CAP and AC (ρ=0.646. P<0.001). The univariable regression analysis also showed a significant association, with the following regression equation being estimated: AC=0.001925×CAP+0.195454 (Fig. 1). The multivariable regression analysis, which included sex (as a variable that showed a significant association with CAP in the univariable analysis), obesity, and ALT as covariates, also showed a significant association between CAP and AC (P<0.001) (Table 2).

Fig. 1.

Scatterplot with linear regression line for the attenuation coefficient in relation to the controlled attenuation parameter (CAP).

There was a moderate-to-good correlation between the attenuation coefficient and CAP (ρ=0.646, P<0.001).

Table 2.

Multivariable linear regression analysis assessing the strength of the association between the attenuation coefficient and controlled attenuation parameter

Variable		Univariable analysis			Multivariable analysis
		Regression coefficient (standard error)	95% CI	P-value	Regression coefficient (standard error)	95% CI	P-value
Main factor
	Attenuation coefficient (dB/cm/MHz)	279.900 (31.410)	217.231 to 342.573	<0.001	299.815 (33.717)	232.437 to 367.192	<0.001
Covariates
	Age (year)	3.235 (1.963)	-0.680 to 7.151	0.104
	Sex
	Female	Reference category			Reference category
	Male	27.240 (13.420)	0.455 to 54.016	0.046	23.527 (9.652)	4.240 to 42.814	0.018
	BMI (kg/m2)	1.982 (1.373)	-0.758 to 4.721	0.153
	<30 kg/m2	Reference category
	≥30 kg/m2	21.049 (13.721)	-6.339 to 48.437	0.130
BMI percentile
	Normal (<85th percentile)	Reference category			Reference category
	Overweight (85th–94th percentile)	26.910 (17.980)	-8.991 to 62.819	0.139	-3.691 (12.429)	-28.529 to 21.147	0.768
	Obesity (95th–98th percentile)	25.030 (22.430)	-19.748 to 69.815	0.268	-1.583 (15.198)	-31.953 to 28.787	0.917
	Severe obesity (≥99th percentile)	17.630 (15.360)	-13.035 to 48.285	0.255	14.952 (10.494)	-6.018 to 35.922	0.159
ALT (IU/L)		0.085 (0.061)	-0.036 to 0.207	0.166	-0.066 (0.044)	-0.155 to 0.023	0.144
AST (IU/L)		0.102 (0.142)	-0.182 to 0.385	0.476
r-GT (IU/L)		0.199 (0.170)	–0.145 to 0.542	0.249
Fasting glucose (mg/dL)		0.550 (0.534)	–0.516 to 1.616	0.307
Triglycerides (mg/dL)		0.026 (0.091)	–0.155 to 0.208	0.773
LDL (mg/dL)		-0.042 (0.214)	–0.471 to 0.387	0.845
HDL (mg/dL)		-0.028 (0.596)	–1.219 to 1.162	0.962

CI, confidence interval; BMI, body mass index; AST, aspartate transaminase; ALT, alanine transaminase; r-GT, gamma-glutamyl transferase; LDL, low-density lipoprotein; HDL, high-density lipoprotein.

The results of the subgroup analyses are summarized in Table 3. Significant correlations between CAP and AC were still found when the patients were divided into subgroups according to sex and ALT. However, when the patients were dichotomized according to BMI, a correlation between CAP and AC was not evident in patients with a BMI ≥30 kg/m2 (ρ=-0.202, P=0.551). Indeed, when those patients were excluded, the Spearman rank correlation coefficient in patients with a BMI <30 kg/m2 increased to 0.730 (P<0.001).

Table 3.

Subgroup analysis of the correlation between the attenuation coefficient and controlled attenuation parameter

Subgroup	Spearman correlation coefficient	P-value
Sex
Female	0.779	0.003
Male	0.610	<0.001
BMI (kg/m2)
<30	0.730	<0.001
≥30	-0.202	0.551
BMI percentile
Normal (<85th percentile)	0.772	0.009
Overweight (85th–94th percentile)	0.738	0.003
Obesity (95th–98th percentile)	0.912	0.011
Severe obesity (≥99th percentile)	0.544	<0.001
ALT
Normal	0.850	0.007
Abnormal[a)]	0.548	<0.001

BMI, body mass index; ALT, alanine transaminase.

Abnormal ALT was defined as elevated ALT (≥52 IU/L for boys, ≥44 IU/L for girls) more than twice the upper limit of normal (26 IU/L for boys, 22 IU/L for girls).

Diagnostic Accuracy

According to the univariable linear regression model for the relationship between AC and CAP, the AC value corresponding to 241 dB/m (the suggested CAP cutoff for the diagnosis of fatty liver) was 0.66 dB/cm/MHz. In addition, the AC values corresponding to 299 dB/m (the suggested CAP cutoff for discriminating S0-1 from S2-3) and 303 dB/m (the suggested CAP cutoff for discriminating S0-2 from S3) were 0.77 and 0.78 dB/cm/MHz, respectively. When an AC value of 0.66 dB/cm/MHz was adopted as the cutoff value for the diagnosis of fatty liver, the sensitivity and specificity of AC were 0.93 (95% confidence interval [CI], 0.85 to 0.98) and 0.87 (95% CI, 0.56 to 1.00), respectively (Table 4). Regarding the other variables, the sensitivity and specificity were 0.99 (95% CI, 0.95 to 1.00) and 1.00 (1.00 to 1.00) for CAP, 0.69 (95% CI, 0.56 to 0.79) and 0.63 (95% CI, 0.42 to 0.88) for obesity or severe obesity, and 0.75 (95% CI, 0.63 to 0.85) and 0.86 (95% CI, 0.55 to 0.99) for ALT, respectively.

Table 4.

Diagnostic accuracy of the dichotomized CAP, ATI, obesity or severe obesity, and ALT in the diagnosis of fatty liver

Index test	Cutoff value	Sensitivity (95% CI)	Specificity (95% CI)	Accuracy (95% CI)
CAP	241 dB/m	0.99 (0.95–1.00)	1.00 (1.00–1.00)	0.99 (0.92–1.00)
ATI	0.66 dB/cm/MHz	0.93 (0.85–0.98)	0.87 (0.56–1.00)	0.93 (0.84–0.97)
Obesity or severe obesity	BMI ≥95th percentile	0.69 (0.56–0.79)	0.63 (0.42–0.88)	0.69 (0.57–0.78)
ALT	52 IU/L for boys, 44 IU/L for girls	0.75 (0.63–0.85)	0.86 (0.55–0.99)	0.76 (0.64–0.84)

Diagnostic accuracy was estimated using the imperfect gold-standard methodology based on a two-level Bayesian latent class model.

CAP, controlled attenuation parameter; ATI, attenuation imaging; ALT, alanine aminotransferase; CI, confidence interval.

Discussion

The present study demonstrated that ATI is a good alternative to the FibroScan vibration-controlled transient elastography CAP measurement for the diagnosis of fatty liver in a pediatric population. A moderate-to-good correlation was found between AC and CAP, as confirmed by multivariable regression analysis. In addition, ATI with an AC cutoff value of 0.66 dB/cm/MHz was accurate for the diagnosis of fatty liver.

The diagnostic performance of ATI in a pediatric population in this study was comparable to the values reported in adult populations [15,16,27,28]. A previous study that included 48 pediatric patients also showed that AC had good diagnostic performance in comparison with a reference standard of MRI-PDFF, with a sensitivity of 0.80 and a specificity of 0.98 using an AC threshold of 0.60 dB/cm/MHz [29]. In a meta-analysis of adult populations, the pooled sensitivity and specificity of AC were 0.76 and 0.84, respectively [28].

Compared with the imaging modalities available for adults, those available for diagnosing fatty liver in children are limited. Although CT is easy to perform and widely used in adults, it should be cautiously used in children because of its relative radiation hazard [30,31]. MRI is a valuable noninvasive imaging tool for the detection and quantification of liver fat and does not pose a risk of exposure to ionizing radiation. However, sedation is generally required for young children. Furthermore, MRI is expensive and requires dedicated sequences for fat quantification. Liver biopsy is, needless to say, invasive and unsuitable for screening purposes. By contrast, US is a simple radiation-free inexpensive modality. Considering this, it is important to demonstrate the diagnostic performance of ATI for fatty liver.

ATI has several advantages over FibroScan CAP. First, ATI can be performed in one sitting along with the main abdominal US examination, whereas CAP can only be obtained using a FibroScan machine, which does not provide any grayscale imaging. Second, the ROI for ATI measurement can be placed in an appropriate area, avoiding unreliable structures and lesions such as hepatic vessels, biliary tree, gallbladder, and ascites. Third, ATI can measure AC differently in cases of uneven hepatic fat infiltration. Last, because of its inexpensiveness and simplicity, ATI is suitable for repetitive monitoring.

In addition, AC is theoretically less affected by tissue outside of the ROI because AC is estimated by calculating the spatial rate of ultrasound energy loss in tissue limited to the ROI [19]. Bae et al. [15] also reported that AC was not statistically associated with the skin-to-liver capsule distance in their multivariable analysis. Furthermore, the intra-observer reliability of AC was reported to be excellent independently of the skin-to-liver capsule distance, although a sensitivity analysis of interobserver reliability was not available [32]. In contrast, it has been reported that CAP has a positive correlation with abdominal wall thickness [12]. In the present study, there was a poor correlation between CAP and ATI in patients with a BMI ≥30 kg/m2; indeed, FibroScan measurement failure was more frequently observed in obese patients, especially when using the M probe [33,34]. Furthermore, CAP was overestimated in patients with a large skin-to-capsular distance, leading to inferior diagnostic accuracy for the diagnosis of hepatic steatosis [35]. In addition, one study showed that interobserver agreement was inversely correlated with BMI [36]. As obesity has an apparent correlation with NAFLD [37], patients with suspected fatty liver tend to be obese and have a thick abdominal wall. Therefore, ATI may be more accurate in very obese patients. However, further research is necessary to clarify this point.

The present study has several limitations of note. First, the number of included patients was modest, limiting the statistical power. Second, a robust reference standard such as liver biopsy or MRI was not obtained. Instead, this study used the imperfect gold-standard methodology based on a two-level Bayesian latent class model. However, this method generates results for the "relative" sensitivity and specificity in comparison with other index tests included in the model [38]. Considering that FibroScan CAP is not a perfect diagnostic test, the calculated sensitivity and specificity of ATI would be inevitably overestimated. Further validation studies with the use of a robust standard are mandatory. Third, the diagnostic performance of ATI for detection of moderate-to-severe or severe degree of fatty liver was not determined. Fourth, an intra- or inter-reader reliability analysis was not conducted. Measurements may be particularly difficult in children because of their irregular breathing and poor cooperation. Further research with a larger sample size and a robust reference standard seems mandatory to clarify these issues. Fifth, although the persistence of elevated ALT levels (>3 months) is emphasized for the screening of pediatric fatty liver, only the ALT values at the time of US exams were used in the present study due to the unavailability of previous ALT values in general.

In conclusion, ATI showed an acceptable correlation with CAP values in a pediatric population, especially in patients with a BMI <30 kg/m2. An AC cutoff of 0.66 dB/cm/MHz, corresponding to a CAP of 241 dB/m, can accurately diagnose liver steatosis.