Kazuyuki Hamada1,2,3, Yutaro Kubota4,2,3, Yusuke Aoki4,2, Norihiko Sugisawa4,2, Jun Yamamoto4,2, Yoshihiko Tashiro4,2, Michael Bouvet2, Takuya Tsunoda5, Robert M Hoffman6,2. 1. AntiCancer Inc, San Diego, CA, U.S.A.; hamadakaz@med.showa-u.ac.jp. 2. Department of Surgery, University of California, San Diego, CA, U.S.A. 3. Division of Medical Oncology, Department of Medicine, Showa University School of Medicine, Tokyo, Japan. 4. AntiCancer Inc, San Diego, CA, U.S.A. 5. Division of Medical Oncology, Department of Medicine, Showa University School of Medicine, Tokyo, Japan ttsunoda@med.showa-u.ac.jp. 6. AntiCancer Inc, San Diego, CA, U.S.A.; all@anticancer.com.
Abstract
BACKGROUND/AIM: Obesity is a major risk factor for colorectal cancer. The MC38 mouse colon-cancer cell line is a versatile syngeneic model of colon cancer in C57BL/6 mice. In the present study, the influence of a high-fat diet (HFD) on the growth of the MC38 mouse colon-cancer cell line was examined in an orthotopic-transplantation syngeneic model in C57BL/6 mice. MATERIALS AND METHODS: Five 6-week-old C57BL/6 male mice were fed a control diet (CD, 6.5% fat) or HFD (34.3% fat) for eight weeks. Then, a 2 mm3 fragment of a subcutaneous MC38 tumor was attached to the surface of the cecum of C57BL/6 mice with a single stitch using a 7-0 suture to establish an orthotopic-transplantation model. Each group continued their initial diet for 17 days. RESULTS: The HFD group had more than twice the tumor volume and tumor weight than the CD group (p=0.021 and p=0.014, respectively). CONCLUSION: HFD-induced obesity strongly increased MC38 colon-cancer progression in a C57BL/6 orthotopic-transplantation mouse model. The present study emphasizes the detrimental effect of obesity on coloncancer progression.
BACKGROUND/AIM: Obesity is a major risk factor for colorectal cancer. The MC38 mouse colon-cancer cell line is a versatile syngeneic model of colon cancer in C57BL/6 mice. In the present study, the influence of a high-fat diet (HFD) on the growth of the MC38 mouse colon-cancer cell line was examined in an orthotopic-transplantation syngeneic model in C57BL/6 mice. MATERIALS AND METHODS: Five 6-week-old C57BL/6 male mice were fed a control diet (CD, 6.5% fat) or HFD (34.3% fat) for eight weeks. Then, a 2 mm3 fragment of a subcutaneous MC38 tumor was attached to the surface of the cecum of C57BL/6 mice with a single stitch using a 7-0 suture to establish an orthotopic-transplantation model. Each group continued their initial diet for 17 days. RESULTS: The HFD group had more than twice the tumor volume and tumor weight than the CD group (p=0.021 and p=0.014, respectively). CONCLUSION: HFD-induced obesity strongly increased MC38 colon-cancer progression in a C57BL/6 orthotopic-transplantation mouse model. The present study emphasizes the detrimental effect of obesity on coloncancer progression.
Authors: Yoshihiko Tashiro; Qinghong Han; Yuying Tan; Norihiko Sugisawa; Jun Yamamoto; Hiroto Nishino; Sachiko Inubushi; Y U Sun; Hyein Lim; Takeshi Aoki; Masahiko Murakami; Yoshihisa Takahashi; Michael Bouvet; Robert M Hoffman Journal: In Vivo Date: 2020 May-Jun Impact factor: 2.155
Authors: Alison E Ringel; Jefte M Drijvers; Gregory J Baker; Alessia Catozzi; Juan C García-Cañaveras; Brandon M Gassaway; Brian C Miller; Vikram R Juneja; Thao H Nguyen; Shakchhi Joshi; Cong-Hui Yao; Haejin Yoon; Peter T Sage; Martin W LaFleur; Justin D Trombley; Connor A Jacobson; Zoltan Maliga; Steven P Gygi; Peter K Sorger; Joshua D Rabinowitz; Arlene H Sharpe; Marcia C Haigis Journal: Cell Date: 2020-12-09 Impact factor: 41.582
Authors: Ruifang Li; Sara A Grimm; Deepak Mav; Haiwei Gu; Danijel Djukovic; Ruchir Shah; B Alex Merrick; Daniel Raftery; Paul A Wade Journal: Cell Rep Date: 2018-01-16 Impact factor: 9.423