| Literature DB >> 35727444 |
Joanna C Sadler1,2,3,4,5, Neil Swainston6, Mark S Dunstan1,3,4, Andrew Currin6, Douglas B Kell7,8.
Abstract
Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as kcat, thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein's vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.Entities:
Keywords: Automation; Bespoke gene variant library synthesis; Directed evolution; Synthetic biology
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Year: 2022 PMID： 35727444 DOI： 10.1007/978-1-0716-2152-3_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745