Vallabhaneni Tilak Chowdary1, V Manoj Kumar2, P Kishore Varma3, B Sreekanth4, V Srinivas Rao5, R Selvarajan6, V Balasubramanian7. 1. Department of Plant Pathology, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India. tilakvallabhaneni@gmail.com. 2. Department of Plant Pathology, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India. 3. Department of Plant pathology, RARS, Anakapalle, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India. 4. Department of Crop Physiology, RARS, Lam, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India. 5. Department of Statistics and Computer applications, Agricultural College, Bapatla, Acharya N.G. Ranga Agricultural University, Guntur, Andhra Pradesh, India. 6. Molecular Virology Lab, Division of Crop Protection, ICAR National Research Centre for Banana, Thayanur Post, Tiruchirapalli, Tamil Nadu, India. selvarajanr@gmail.com. 7. Molecular Virology Lab, Division of Crop Protection, ICAR National Research Centre for Banana, Thayanur Post, Tiruchirapalli, Tamil Nadu, India.
Abstract
BACKGROUND: Mungbean yellow mosaic India virus (MYMIV) is a representative of the genus begomovirus/Begomoviridae, which is prevalent in the northern part of Indian subcontinent causing yellow mosaic disease (YMD). This virus is rapidly evolving and breaking the resistance in the advanced lines causing huge economic losses in the pulse production. In this context, the present investigation on characterization of the causal organism of YMD was undertaken METHODS AND RESULTS: A novel recombinant isolate (YMV-BG-BPT) causing YMD was identified from blackgram in Andhra Pradesh, southern peninsular region of India. The association of a bipartite begomovirus with the disease was done by sequence analyses of the cloned full-length genome. The full length genome sequences were submitted in NCBI GenBank with accession numbers MZ235792 (DNA-A) and MZ356197 (DNA-B). The sequence analysis of DNA-A of YMV-BG-BPT showed maximum of 99.12% similarity at nucleotide level with Mungbean yellow mosaic India virus (MYMIV) isolate reported from Tamil Nadu (KC911719), India which is also confirmed by clustering pattern in phylogenic analysis and DNA-B showed 95.79% with Mungbean yellow mosaic virus (MYMV) isolate reported from Tamil Nadu (KP319016) and 95.05% with MYMIV isolate reported from Karnataka (MT027037). The huge variation in DNA-B lead us to suspect a recombination in DNA-B, where a recombination event in the CR, region coding for nuclear shuttle protein and movement protein of DNA B was detected in which MYMV-BG-AP-IND (KF928962) and MYMIV-GG-CH-IND (MN020536) have been identified as major and minor parents, respectively. CONCLUSION: Overall, the present study revealed occurrence of MYMIV with recombinant DNA B component in southern peneinsular India.
BACKGROUND: Mungbean yellow mosaic India virus (MYMIV) is a representative of the genus begomovirus/Begomoviridae, which is prevalent in the northern part of Indian subcontinent causing yellow mosaic disease (YMD). This virus is rapidly evolving and breaking the resistance in the advanced lines causing huge economic losses in the pulse production. In this context, the present investigation on characterization of the causal organism of YMD was undertaken METHODS AND RESULTS: A novel recombinant isolate (YMV-BG-BPT) causing YMD was identified from blackgram in Andhra Pradesh, southern peninsular region of India. The association of a bipartite begomovirus with the disease was done by sequence analyses of the cloned full-length genome. The full length genome sequences were submitted in NCBI GenBank with accession numbers MZ235792 (DNA-A) and MZ356197 (DNA-B). The sequence analysis of DNA-A of YMV-BG-BPT showed maximum of 99.12% similarity at nucleotide level with Mungbean yellow mosaic India virus (MYMIV) isolate reported from Tamil Nadu (KC911719), India which is also confirmed by clustering pattern in phylogenic analysis and DNA-B showed 95.79% with Mungbean yellow mosaic virus (MYMV) isolate reported from Tamil Nadu (KP319016) and 95.05% with MYMIV isolate reported from Karnataka (MT027037). The huge variation in DNA-B lead us to suspect a recombination in DNA-B, where a recombination event in the CR, region coding for nuclear shuttle protein and movement protein of DNA B was detected in which MYMV-BG-AP-IND (KF928962) and MYMIV-GG-CH-IND (MN020536) have been identified as major and minor parents, respectively. CONCLUSION: Overall, the present study revealed occurrence of MYMIV with recombinant DNA B component in southern peneinsular India.
Authors: Judith K Brown; F Murilo Zerbini; Jesús Navas-Castillo; Enrique Moriones; Roberto Ramos-Sobrinho; José C F Silva; Elvira Fiallo-Olivé; Rob W Briddon; Cecilia Hernández-Zepeda; Ali Idris; V G Malathi; Darren P Martin; Rafael Rivera-Bustamante; Shigenori Ueda; Arvind Varsani Journal: Arch Virol Date: 2015-04-18 Impact factor: 2.574
Authors: G R Argüello-Astorga; R G Guevara-González; L R Herrera-Estrella; R F Rivera-Bustamante Journal: Virology Date: 1994-08-15 Impact factor: 3.616
Authors: A S Karthikeyan; R Vanitharani; V Balaji; S Anuradha; P Thillaichidambaram; P V Shivaprasad; C Parameswari; V Balamani; M Saminathan; K Veluthambi Journal: Arch Virol Date: 2004-04-05 Impact factor: 2.574