Literature DB >> 3571480

Rapid screening assay for phospholipase C activity in mycoplasmas.

N S De Silva, P A Quinn.   

Abstract

A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presence in Ureaplasma urealyticum of phospholipase C, which is predominantly localized in the membrane fraction. The procedure has the potential to screen phospholipase C activity in other mycoplasmas and microorganisms in general.

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Year:  1987        PMID： 3571480      PMCID： PMC266069          DOI： 10.1128/jcm.25.4.729-731.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Phospholipase C assay using p-nitrophenylphosphoryl-choline together with sorbitol and its application to studying the metal and detergent requirement of the enzyme.

Authors:  S Kurioka; M Matsuda
Journal:  Anal Biochem       Date:  1976-09       Impact factor: 3.365

3.  The egg yolk reaction produced by several species of bacteria.

Authors:  J J Owens
Journal:  J Appl Bacteriol       Date:  1974-03

4.  Thin-layer chromatographic technique for rapid detection of bacterial phospholipases.

Authors:  N J Legakis; J Papavassiliou
Journal:  J Clin Microbiol       Date:  1975-11       Impact factor: 5.948

5.  Exotoxin activity associated with the Legionnaires disease bacterium.

Authors:  W B Baine; J K Rasheed; D C Mackel; C A Bopp; J G Wells; A F Kaufmann
Journal:  J Clin Microbiol       Date:  1979-03       Impact factor: 5.948

6.  Premature labor. II. Bacterial sources of phospholipase.

Authors:  R Bejar; V Curbelo; C Davis; L Gluck
Journal:  Obstet Gynecol       Date:  1981-04       Impact factor: 7.661

7.  Endogenous activity of phospholipases A and C in Ureaplasma urealyticum.

Authors:  N S De Silva; P A Quinn
Journal:  J Clin Microbiol       Date:  1986-02       Impact factor: 5.948

8.  Enzyme-linked immunosorbent assay for detection of specific antibodies to Ureaplasma urealyticum serotypes.

Authors:  C A Wiley; P A Quinn
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948
  8 in total
  7 in total

1.  Characterization of phospholipase A1, A2, C activity in Ureaplasma urealyticum membranes.

Authors:  N S DeSilva; P A Quinn
Journal:  Mol Cell Biochem       Date:  1999-11       Impact factor: 3.396

Review 2.  Potential role of phospholipases in virulence and fungal pathogenesis.

Authors:  M A Ghannoum
Journal:  Clin Microbiol Rev       Date:  2000-01       Impact factor: 26.132

3.  Synthesis and secretion of phospholipase C by oral spirochetes.

Authors:  R Siboo; W al-Joburi; M Gornitsky; E C Chan
Journal:  J Clin Microbiol       Date:  1989-03       Impact factor: 5.948

Review 4.  Bacterial phospholipases C.

Authors:  R W Titball
Journal:  Microbiol Rev       Date:  1993-06

5.  GPR83 engages endogenous peptides from two distinct precursors to elicit differential signaling.

Authors:  Seshat M Mack; Ivone Gomes; Amanda K Fakira; Mariana L Duarte; Achla Gupta; Lloyd Fricker; Lakshmi A Devi
Journal:  Mol Pharmacol       Date:  2022-05-23       Impact factor: 4.054

6.  Identification of GPR83 as the receptor for the neuroendocrine peptide PEN.

Authors:  Ivone Gomes; Erin N Bobeck; Elyssa B Margolis; Achla Gupta; Salvador Sierra; Amanda K Fakira; Wakako Fujita; Timo D Müller; Anne Müller; Matthias H Tschöp; Gunnar Kleinau; Lloyd D Fricker; Lakshmi A Devi
Journal:  Sci Signal       Date:  2016-04-26       Impact factor: 8.192

7.  Comparative genome analysis of 19 Ureaplasma urealyticum and Ureaplasma parvum strains.

Authors:  Vanya Paralanov; Jin Lu; Lynn B Duffy; Donna M Crabb; Susmita Shrivastava; Barbara A Methé; Jason Inman; Shibu Yooseph; Li Xiao; Gail H Cassell; Ken B Waites; John I Glass
Journal:  BMC Microbiol       Date:  2012-05-30       Impact factor: 3.605
  7 in total

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