| Literature DB >> 35712867 |
Jian Chen1,2,3, Chenxu Gao1,2,3,4, Mengcheng Luo5, Chunwei Zheng1,2,3, Xiwen Lin1,2,3, Yan Ning1,2,3,4, Longfei Ma1,2,3,4, Wei He1,2,3,4, Dan Xie1,2,3,4, Kui Liu6,7, Kai Hong8, Chunsheng Han1,2,3,4.
Abstract
MicroRNAs (miRNAs) are believed to play important roles in mammalian spermatogenesis but the in vivo functions of single miRNAs in this highly complex developmental process remain unclear. Here, we report that miR-202, a member of the let-7 family, plays an important role in spermatogenesis by phenotypic evaluation of miR-202 knockout (KO) mice. Loss of miR-202 results in spermatocyte apoptosis and perturbation of the zygonema-to-pachynema transition. Multiple processes during meiosis prophase I including synapsis and crossover formation are disrupted, and inter-sister chromatid synapses are detected. Moreover, we demonstrate that Separase mRNA is a miR-202 direct target and provides evidence that miR-202 upregulates REC8 by repressing Separase expression. Therefore, we have identified miR-202 as a new regulating noncoding gene that acts on the established SEPARASE-REC8 axis in meiosis.Entities:
Keywords: zzm321990miR-202zzm321990; REC8; SEPARASE; meiosis prophase I; spermatogenesis
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Year: 2022 PMID： 35712867 PMCID： PMC9346496 DOI： 10.15252/embr.202154298
Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 9.071