| Literature DB >> 35710609 |
Piero Giansanti1, Patroklos Samaras1, Yangyang Bian1,2, Chen Meng3, Andrea Coluccio4,5,6,7, Martin Frejno1, Hannah Jakubowsky4,5,6,7, Sophie Dobiasch8,9,10,11, Rashmi R Hazarika12,13, Julia Rechenberger1, Julia Calzada-Wack14, Johannes Krumm1, Sebastian Mueller6,7,15, Chien-Yun Lee1, Nicole Wimberger4,5,6,7, Ludwig Lautenbacher1, Zonera Hassan16, Yun-Chien Chang1, Chiara Falcomatà4,5,6,7, Florian P Bayer1, Stefanie Bärthel4,5,6,7, Tobias Schmidt1, Roland Rad4,6,7,15, Stephanie E Combs8,9,10,11, Matthew The1, Frank Johannes12,13, Dieter Saur4,5,6,7, Martin Hrabe de Angelis14,17,18, Mathias Wilhelm1,19, Günter Schneider16,20, Bernhard Kuster21,22,23,24,25.
Abstract
The laboratory mouse ranks among the most important experimental systems for biomedical research and molecular reference maps of such models are essential informational tools. Here, we present a quantitative draft of the mouse proteome and phosphoproteome constructed from 41 healthy tissues and several lines of analyses exemplify which insights can be gleaned from the data. For instance, tissue- and cell-type resolved profiles provide protein evidence for the expression of 17,000 genes, thousands of isoforms and 50,000 phosphorylation sites in vivo. Proteogenomic comparison of mouse, human and Arabidopsis reveal common and distinct mechanisms of gene expression regulation and, despite many similarities, numerous differentially abundant orthologs that likely serve species-specific functions. We leverage the mouse proteome by integrating phenotypic drug (n > 400) and radiation response data with the proteomes of 66 pancreatic ductal adenocarcinoma (PDAC) cell lines to reveal molecular markers for sensitivity and resistance. This unique atlas complements other molecular resources for the mouse and can be explored online via ProteomicsDB and PACiFIC.Entities:
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Year: 2022 PMID： 35710609 PMCID： PMC7613032 DOI： 10.1038/s41592-022-01526-y
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 47.990