| Literature DB >> 35687227 |
Songon An1,2, Prakash Parajuli3, Erin L Kennedy3, Minjoung Kyoung4,5.
Abstract
Fluorescence live-cell imaging that has contributed to our understanding of cell biology is now at the frontline of studying quantitative biochemistry in a cell. Particularly, technological advancements of fluorescence live-cell imaging and associated strategies in recent years have allowed us to discover various subcellular macromolecular assemblies in living human cells. Here we describe how real-time dynamics of a multienzyme metabolic assembly, the "glucosome," that is responsible for regulating glucose flux at subcellular levels, has been investigated in both 2- and 3-dimensional space of single human cells. We envision that such multi-dimensional fluorescence live-cell imaging will continue to revolutionize our understanding of how intracellular metabolic pathways and their network are functionally orchestrated at single-cell levels.Entities:
Keywords: Cancer Metabolism; Fluorescence Microscopy; Glucose Metabolism; Glucosome; Glycolysis; Lattice Light-sheet Microscopy; Live-cell Imaging; Macromolecular Assembly; Metabolic Complex; Metabolic Condensate
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Year: 2022 PMID： 35687227 PMCID： PMC9191769 DOI： 10.1007/978-1-0716-2269-8_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745