Literature DB >> 356854

Solubilization of bovine heart-value collagen.

S A Jimenez, R I Bashey.   

Abstract

Complete solubilization of bovine heart-valve gollagen was obtained by using sequential pepsin digestion and extraction with dithiothreitol under non-denaturing conditions. Use of the reducing agent was the crucial step resulting in solubilization. These findings suggest that disulphide-bonded proteins may be in part responsible for the insolubility of bovine heart-valve collagen.

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Year:  1978        PMID： 356854      PMCID： PMC1185779          DOI： 10.1042/bj1730337

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid.

Authors:  J F WOESSNER
Journal:  Arch Biochem Biophys       Date:  1961-05       Impact factor: 4.013

2.  Chromatographic determination of cystine as cysteic acid.

Authors:  E SCHRAM; S MOORE; E J BIGWOOD
Journal:  Biochem J       Date:  1954-05       Impact factor: 3.857

3.  Amino acid composition of bovine heart valve collagen.

Authors:  R I Bashey; Y Mori; L Prosky
Journal:  Proc Soc Exp Biol Med       Date:  1972-07

4.  Identification of collagen alpha1(I) trimer in embryonic chick tendons and calvaria.

Authors:  S A Jimenez; R I Bashey; M Benditt; R Yankowski
Journal:  Biochem Biophys Res Commun       Date:  1977-10-24       Impact factor: 3.575

5.  Age-related collagen and elastin content of human heart valves.

Authors:  R I Bashey; S Torii; A Angrist
Journal:  J Gerontol       Date:  1967-04
  5 in total
  4 in total

1.  Collagenous membrane from the surface of human visceral pleura.

Authors:  B A Bray; S Keller; I Mandl; G M Turino
Journal:  Lung       Date:  1985       Impact factor: 2.584

2.  Collagen composition of normal and myxomatous human mitral heart valves.

Authors:  W G Cole; D Chan; A J Hickey; D E Wilcken
Journal:  Biochem J       Date:  1984-04-15       Impact factor: 3.857

3.  Biochemical characterization of individual normal, floppy and rheumatic human mitral valves.

Authors:  Y Lis; M C Burleigh; D J Parker; A H Child; J Hogg; M J Davies
Journal:  Biochem J       Date:  1987-06-15       Impact factor: 3.857

4.  Quantitative analysis of type X-collagen biosynthesis by embryonic-chick sternal cartilage.

Authors:  S A Jimenez; R Yankowski; A M Reginato
Journal:  Biochem J       Date:  1986-01-15       Impact factor: 3.857
  4 in total

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