Literature DB >> 356727

Fluorescent assay for estimating the binding of erythromycin derivatives to ribosomes.

P Brandt-Rauf, R Vince, R LeMahieu, S Pestka.   

Abstract

A fluorescent erythromycin derivative was used to determine the binding of erythromycin derivatives to ribosomes. The assay is rapid, sensitive, and convenient. Because measurements are made in solution, they represent equilibrium conditions, unlike filter binding assays which perturb the equilibrium. Results correlate well with measurements made by other techniques.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 356727      PMCID： PMC352409          DOI： 10.1128/AAC.14.1.88

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  10 in total

1.  Effect of leucomycins and analogues on binding [14C ]erythromycin to Escherichia coli ribosomes.

Authors:  S Pestka; A Nakagawa; S Omura
Journal:  Antimicrob Agents Chemother       Date:  1974-11       Impact factor: 5.191

2.  Interaction between the erythromycin and chloramphenicol binding sites on the Escherichica coli ribosome.

Authors:  R Langlois; C R Cantor; R Vince; S Pestka
Journal:  Biochemistry       Date:  1977-05-31       Impact factor: 3.162

3.  Studies on the formation of transfer ribonucleic acid-ribosome complexes. XXIV. Effects of antibiotics on binding of aminoacyl-oligonucleotides to ribosomes.

Authors:  R Harris; S Pestka
Journal:  J Biol Chem       Date:  1973-02-25       Impact factor: 5.157

4.  Studies on the formation of transfer ribonucleic acid-ribosome complexes. 3. The formation of peptide bonds by ribosomes in the absence of supernatant enzymes.

Authors:  S Pestka
Journal:  J Biol Chem       Date:  1968-05-25       Impact factor: 5.157

5.  The distance between two functionally significant regions of the 50 S Escherichia coli ribosome: the erythromycin binding site and proteins L7/L12.

Authors:  R Langlois; C C Lee; C R Cantor; R Vince; S Pestka
Journal:  J Mol Biol       Date:  1976-09-15       Impact factor: 5.469

6.  Structure-activity relationships among the O-acyl derivatives of leucomycin. Correlation of minimal inhibitory concentrations with binding to Escherichia coli ribosomes.

Authors:  S Omura; A Nakagawa; H Sakakibara; O Okekawa; R Brandsch
Journal:  J Med Chem       Date:  1977-05       Impact factor: 7.446

7.  Effect of erythromycin analogues on binding of [14C]erythromycin to Escherichia coli ribosomes.

Authors:  S Pestka; R A Lemahieu
Journal:  Antimicrob Agents Chemother       Date:  1974-10       Impact factor: 5.191

8.  Inhibition of [14C]chloramphenicol binding to Escherichia coli ribosomes by erythromycin derivatives.

Authors:  S Pestka; R A LeMahieu
Journal:  Antimicrob Agents Chemother       Date:  1974-07       Impact factor: 5.191

9.  Aromatic esters of 5-O-desosaminylerythronolide A oxime.

Authors:  R A LeMahieu; M Carson; R W Kierstead; S Pestka
Journal:  J Med Chem       Date:  1975-08       Impact factor: 7.446

10.  Binding of N-substituted erythromycyclamines to ribosomes.

Authors:  R Vince; D Weiss; S Pestka
Journal:  Antimicrob Agents Chemother       Date:  1976-01       Impact factor: 5.191
  10 in total
  2 in total

1.  Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding.

Authors:  Beatriz Llano-Sotelo; Robyn P Hickerson; Laura Lancaster; Harry F Noller; Alexander S Mankin
Journal:  RNA       Date:  2009-06-24       Impact factor: 4.942

2.  Fluorescence polarization method to characterize macrolide-ribosome interactions.

Authors:  Kang Yan; Eric Hunt; John Berge; Earl May; Robert A Copeland; Richard R Gontarek
Journal:  Antimicrob Agents Chemother       Date:  2005-08       Impact factor: 5.191
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.