| Literature DB >> 35622329 |
Dian Kortleve1, Mandy van Brakel2, Rebecca Wijers2, Reno Debets2, Dora Hammerl2.
Abstract
Prior to clinical testing of adoptive T-cell therapy with T-cell receptor (TCR)-engineered T cells, TCRs need to be retrieved, annotated, gene-transferred, and extensively tested in vitro to accurately assess specificity and sensitivity of target recognition. Here, we present a fundamental series of protocols that cover critical preclinical parameters, thereby enabling the selection of candidate TCRs for clinical testing.Entities:
Keywords: Gene transfer; In vitro assays; Sensitivity; Specificity; T-cell engineering; T-cell receptor; TCR annotation; TCR cloning
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Year: 2022 PMID： 35622329 DOI： 10.1007/978-1-0716-2115-8_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745