| Literature DB >> 35616855 |
Tim H Szeto1, Pascal M W Drake2, Audrey Y-H Teh1, Nicole Falci Finardi1, Ashleigh G Clegg1, Mathew J Paul1, Rajko Reljic1, Julian K-C Ma1.
Abstract
Nicotiana tabacum (the tobacco plant ) has numerous advantages for molecular farming, including rapid growth, large biomass and the possibility of both cross- and self-fertilization. In addition, genetic transformation and tissue culture protocols for regeneration of transgenic plants are well-established. Here, we describe the production of transgenic tobacco using Agrobacterium tumefaciens and the analysis of recombinant proteins, either in crude plant extracts or after purification, by enzyme-linked immunosorbent assays, sodium dodecyl sulfate polyacrylamide gel electrophoresis with western blotting and surface plasmon resonance.Entities:
Keywords: Agrobacterium tumefaciens; ELISA; Nicotiana tabacum; Recombinant protein; SDS PAGE; Surface plasmon resonance; Transgenic; Western blotting
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Year: 2022 PMID： 35616855 DOI： 10.1007/978-1-0716-2241-4_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745