Dong-Hyun Kim1,2, Yea Ji Kim1, Dong-Min Shin1, Jung Hoon Lee2, Sung Gu Han1. 1. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea. 2. Food Research Team, Meat Bank Corporation, Incheon 22650, Korea.
Meat-restructuring technology is a method that partially or completely disassembles
and reforms meat in a different form (Sun,
2009). This technology can improve the quality characteristics (e.g.,
structure, texture, appearance, and nutritional value) of meat products and has been
applied to low-quality cuts of meat to produce value-added meat products (Gadekar et al., 2014). Therefore, the
manufacture of restructured meat products using low-quality meat cuts has economic
advantages in the meat industry (Gurikar et al.,
2014).Semi-dried restructured sausages, one of the restructured meat products, have higher
protein content and lower moisture content than regular sausages (Choi et al., 2016). However, the structural
complexity of semi-dried restructured sausages, composed of meat and other
functional ingredients, has been reported to reduce thermal conductivity and
permeability during the drying process (Han et al.,
2011). Therefore, the manufacture of semi-dried restructured sausages
requires a long time and high energy consumption.The drying process in semi-dried restructured sausage is an important procedure for
determining quality and storage stability, and hot-air drying is a conventionally
used in these products (Aykın Dinçer,
2021; Jin et al., 2017). During
hot air drying, moisture in the internal muscle migrates to the surface of the
product because of heat conduction from the air onto the surface of the meat,
resulting in moisture evaporation (Shi et al.,
2021b). Thus, increased drying time induces tough texture and off-flavor
(Arnau et al., 2007), and deteriorates the
quality of final products such as tenderness, color, flavor, and nutrients (Ran et al., 2019).Moisture content is known to be highly associated with thermal diffusivity, mass
transfer, and specific heat during the food drying process (Mariani et al., 2008; Modi et
al., 2014; Razavi and Taghizadeh,
2007). In particular, the high initial moisture content of meat products
attenuates the particle-particle interaction in materials, thereby resulting in
increased porosity and shortened drying time (Deng
et al., 2018; Jerwanska et al.,
1995). A previous study reported that the high porosity of products
improves heat and mass transfer during the drying process (Datta, 2007). In addition, the increase in porosity through the
use of various processing methods, such as vacuum, freeze, microwave, and infrared
radiation treatments, improved the drying characteristics and quality properties
(Aykın Dinçer, 2021; Kumar and Karim, 2019; Riadh et al., 2015). Furthermore, the formation of a porous
structure in dried meat products inhibits the shrinkage and tough texture of jerky
(Kim et al., 2021a).We hypothesized that increase of initial moisture contents could improve the drying
rates and physicochemical properties of semi-dried restructured sausages by
increasing porosity and moisture diffusivity. Therefore, this study aimed to
investigate the effects of different initial moisture contents on the drying
characteristics and physicochemical properties of semi-dried restructured
sausages.
Materials and Methods
Preparation of semi-dried restructured sausage
Fresh pork ham was purchased from a local market (Incheon, Korea), and the
visible connective tissue from the meat was trimmed. The lean meat was ground
using a meat grinder (MGB-32, Hankook Fujee Industries, Suwon, Korea) through a
3 mm plate. Six different formulations and the initial moisture contents of the
semi-dried restructured sausages are listed in Table 1. Briefly, sausage samples were prepared using combinations
of pork/water as follows: 100/0%, 90/10%, 80/20%,
70/30%, 60/40%, and 50/50% (w/w) with 1% salt based
on the pork weight (w/w). Ground lean meat and salt were homogenized for 1 min
in a mixer (K5SS, KitchenAid, St. Joseph, MI, USA) and then homogenized with
ice. After homogenization, the meat batter was stuffed into a collagen casing
(10 mm, COLFAN, Lodi, CA, USA) and the length of each sausage was set at 100 mm.
All samples were frozen at −18°C until the core temperature of
each sample reached −15°C, followed by removal of the casing.
Table 1.
Formulation of semi-dried restructured sausages with different water
addition levels
WAL0, 100% pork; WAL10, 90% pork/10% water;
WAL20, 80% pork/20% water; WAL30, 70%
pork/30% water; WAL40, 60% pork/40% water;
WAL50, 50% pork/50% water of semi-dried restructured
sausage.Data are shown as mean±SD.
Drying procedure for semi-dried restructured sausage
The sausages were dried in a convection dry oven (HSC-150, AccuResearch Korea,
Hanam, Korea). The constant air velocity in the dry oven was 0.5±0.1 m/s,
being continuously measured over 3 min. All samples were dried equally at
85°C for different time periods (10, 20, 30, 40, 50, 60, 80, 100, 120,
150, 180, 240, 300, 360, and 580 min). The samples were removed from the oven at
each time point, and the moisture contents were determined according to the
official AOAC method (AOAC, 2000). The
drying kinetics of semi-dried restructured sausage were illustrated using
moisture content (g/g, dry basis), moisture ratio (g/g), drying rate
(g/(g·h)), and effective moisture diffusivity (m2/s) (Xie et al., 2020). All treatment groups had
six replicates and each group weighed approximately 4 kg.
Moisture content
The moisture content of semi-dried restructured sausage at any time was
calculated using the following Equation
(1).where W (g water/ g dry basis) and
W (g) are the weight of sausage at
t time of drying and the final dry weight of sausage which
can be calculated by initial weight and moisture content.
Moisture ratio
The moisture ratio (MR) during the drying experiments can be
expressed by Equation (2).where M0, M, and
M are the initial moisture content (g/g),
moisture content at time t (g/g), and equilibrium moisture
content during the drying process (g/g), respectively. Equation (2) can be simplified to
Equation (3).where, during long drying times, the value of M is
regarded as zero compared to M and
M0 (Aykın-Dinçer and Erbaş, 2018).
Drying rate
The drying rate (DR) can be calculated from the mass of water
removed from the material per unit of time in units of mass. This can be
expressed using Equation
(4).where t1 and t2 are the
specific drying times (min). M
and M, calculated using Equation (1), are the moisture
contents at times t1 and
t2 on a dry basis (g/g), respectively.
Effective moisture diffusivity (D)
The migration of moisture during the drying process is controlled by diffusion.
According to Fick’s second law of diffusion, effective moisture
diffusivity (m2/s) can be calculated when the
moisture content of the semi-dried restructured sausage was reduced below 0.5
g/g (dry basis) using the following Equation (5).Using an infinite slab geometry and consistent initial moisture distribution,
Equation (5) can be
calculated according to Equation
(6) (Aykın-Dinçer and
Erbaş, 2018).where n is the number of experiments, t the
drying time (s), and L the half thickness of the sausage (m).
Equation (6) can be
simplified to Equation (7) by
taking the natural logarithms.The effective moisture diffusivity was calculated by plotting the drying data at
a given drying time using the slope of the graph acquired from
ln(MR). This can be expressed by Equation (8).
Physicochemical characteristics of semi-dried restructured sausage
Sausages with different water contents (0%, 10%, 20%,
30%, 40%, and 50%) were prepared under the same condition
of below 0.5 g/g moisture content on a dry basis. Samples were dried at
85°C for 360, 350, 350, 300, 270, and 270 min using a convection dry oven
(HSC-150). The moisture content of each sample was determined using the AOAC
method (2000). The physicochemical characteristics of the sausages were
determined by measuring the water activity, pH, color, porosity, volatile basic
nitrogen, shear force, and microstructure.
Water activity
A water activity meter (Humimeter RH2, Schaller, Austria) was used to measure the
water activity of semi-dried restructured sausages at a temperature of
25±1°C.
pH value
Semi-dried restructured sausages (5 g) and distilled water (20 mL) were
homogenized using a homogenizer (Daihan Scientific, Gangwon, Korea) for 2 min at
10,000 rpm. The pH of the homogenate was determined using a LAQUA pH meter
(Horiba, Kyoto, Japan).
Color evaluation
The color of the semi-dried restructured sausages was determined using a
colorimeter (CR-210, Minolta Camera, Osaka, Japan). It is expressed using L*
(lightness), a* (redness), and b* (yellowness) color values according to the CIE
(International Commission on Illumination). The colorimeter was calibrated using
a white plate (L*=97.27, a*=5.21, and b*=−3.40).
Porosity
For the calculation of porosity (ε, %), the
apparent density (ρ, g/cm) and real density
(ρ, g/cm) were required (Silva-Espinoza et al., 2019). The apparent
density refers to the weight (m, g) per volume (V,
cm3) of the material, including water and pores. Real density
also refers to the weight per volume but does not consider the pores in the
material. The apparent density can be expressed by Equation (9).where the real density is calculated based on the composition of the sample
according to Equation (10).where X is the mass fraction of water,
ρ the density of water (0.9976
g/cm3), X the mass fraction of
carbohydrates, and ρ the density of
carbohydrates (1.4246 g/cm3). The porosity was calculated using Equation (11).
Shear force analysis
The shear force of the semi-dried restructured sausage was obtained using a
texture analyzer (TA-XT2i, Stable Micro Systems, Surry, UK) with a V-slot blade
(Warner-Bratzler, Hamilton, MA, USA). The texture analyzer was operated at
pre-test speed 2.0 mm/s, test speed 2.0 mm/s and post-test speed 1.0 mm/s at
25±1°C. The shear force was expressed in kilograms (kg).
Volatile basic nitrogen (VBN)
Volatile basic nitrogen (VBN) was measured using microdiffusion analysis as
previously described (Kim et al., 2019).
Semi-dried restructured sausage (5 g) was homogenized with distilled water (20
mL) for 1 min at 12,000 rpm. The homogenates were mixed with distilled water (30
mL) and filtered through Whatman No.1 filter paper. The indicator solution was
prepared by mixing 0.066% methyl red in ethanol and 0.066%
bromocresol green in ethanol at a 1:1 ratio. In the inner part of the Conway
diffusion cell, the indicator solution (100 μL) and 0.01N
H3BO3 (1 mL) were added. The filtrate of the sample (1
mL) and 50% K2CO3 solution (1 mL) were added to the
outer section of the cell. The cells were left at 37°C for 90 min and the
0.02N H2SO4 was titrated to the solution of inner part.
The VBN value is expressed as mg%.
Field emission scanning electron microscopy (FE-SEM) evaluation of
microstructure
The microstructures of the semi-dried restructured sausages were evaluated using
field emission scanning electron microscopy (FE-SEM) (SU8010, Hitachi, Tokyo,
Japan). The samples were cut into small pieces and frozen for 12 h at
−78°C. The frozen samples were lyophilized in a vacuum evaporator
(MCFD 8508, Il Shin Bio, Yangju, Korea) for 24 h. Thereafter, the samples were
sputter-coated with platinum using an ion sputter (MC1000, Hitachi) and observed
at an accelerating voltage of 5 kV by FE-SEM. The magnification of all images
was ×300.
Statistical analysis
Experimental data were analyzed using SPSS Statistics 24 software (IBM, Armonk,
NY, USA). Data are presented as mean±SD, and one-way analysis of variance
with Duncan’s multiple range test was performed to identify significant
differences (p<0.05). Data were obtained from at least three replicates
in all experiments.
Results and Discussion
Moisture content, moisture ratio and drying rate of semi-dried restructured
sausages
The results of moisture content, moisture ratio, and drying rate are shown in
Fig. 1. The moisture content and
moisture ratio of all samples gradually decreased during the drying period
(Fig. 1A and 1B). In addition, the moisture ratio of sausages with water
addition (WAL10−50) was lower than that of the sausages without water
addition (WAL0 group). The drying rate was also higher in sausages with added
water during the initial 10 min of drying (Fig.
1C). Free water evaporates more easily than bound water in food
products (Tunde-Akintunde et al., 2005).
A previous study reported that a higher moisture content of ginger slices
resulted in a higher drying rate during the preliminary stage of drying (Zeng et al., 2022). Thus, the increase of
drying rate resulted from the increase of free water in the sausage. Moreover,
the drying time required to reach a moisture content of 50% (dry basis)
in sausages was shortened by the increase in the water addition level (Table 2). Shortening the drying time during
the production of dried foods is important for minimizing energy consumption and
improving quality properties (Riadh et al.,
2015; Tunde-Akintunde and Ogunlakin,
2011). Our data indicate that an increase of initial moisture content
significantly shortened the drying time and improved the drying characteristics
of the sausages. A similar observation was reported, in which high initial
moisture content using brine injection to beef jerky shortened the drying time
(Kim et al., 2022). Collectively, our
data demonstrate that an increase in the initial water content can accelerate
the drying rate and shorten the drying time in semi-dried restructured
sausages.
Fig. 1.
Moisture content (A), moisture ratio (B), and drying rate curve (C)
of semi-dried restructured sausages with different water addition
levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (10, 20, 30, 40,
50, 60, 80, 100, 120, 150, 180, 240, 300, 360, and 580 min). Moisture
content was calculated as dry basis (g/g, db). The error bars indicate
SD. a–f Different superscript letters represent
significant differences at the drying time (p<0.05).
Table 2.
Effective moisture diffusion coefficient of semi-dried restructured
sausages with different water addition levels during hot air
drying
Different superscript letters represent significant differences
(p<0.05).
Moisture content (A), moisture ratio (B), and drying rate curve (C)
of semi-dried restructured sausages with different water addition
levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (10, 20, 30, 40,
50, 60, 80, 100, 120, 150, 180, 240, 300, 360, and 580 min). Moisture
content was calculated as dry basis (g/g, db). The error bars indicate
SD. a–f Different superscript letters represent
significant differences at the drying time (p<0.05).Data are presented as mean±SD.WAL0, 100% pork; WAL10, 90% pork/10% water;
WAL20, 80% pork/20% water; WAL30, 70%
pork/30% water; WAL40, 60% pork/40% water;
WAL50, 50% pork/50% water of semi-dried restructured
sausage.Different superscript letters represent significant differences
(p<0.05).
Effective moisture diffusivity (D) of
semi-dried restructured sausages
D represents the intrinsic mass transfer in food
materials and has been used to provide information about moisture movement
within foods during the drying process (Dadali
et al., 2007). D values were
calculated at the time of drying, and the moisture content of the sausages
reached 50% (dry basis). The D value of
the sausage increased with the amount of water added (p<0.05) (Table 2). D
is influenced by various factors, such as drying temperature, pretreatment, and
ingredients added to food materials (Sharma and
Prasad, 2004; Tunde-Akintunde and
Ogunlakin, 2011; Wang et al.,
2019). In several previous studies, high moisture content was closely
related to an increase in the D value (Chen et al., 2020; Wang et al., 2019). The D
value is influenced by the thermal conductivity of water, which has a higher
thermal conductivity than proteins or fats (Tavman and Tavman, 1999). Additionally, the pore network, which is
affected by the higher water content, also influences the
D value of foods (Chen, 2007). In a previous study, the porous structure of
pumpkin slices was induced by blanching and freezing before hot air drying,
which resulted in a higher moisture diffusivity during drying (Ando et al., 2019). Collectively, an
increase of initial moisture content positively influenced the high thermal
diffusivity in semi-dried restructured sausages, which consequently led to an
increased drying rate and effective moisture diffusivity.
pH and color of semi-dried restructured sausages
The pH values of the semi-dried restructured sausages are listed in Table 3. Overall, there were no significant
differences between the groups, although an increase of initial moisture content
may be associated with higher pH values. This may result from the shorter drying
time in the groups with added water. According to previous studies, a relatively
short drying time inhibited the lowering of pH in meat products by reducing
protein denaturation during the drying process (Yang et al., 2009).
Table 3.
pH and color of semi-dried restructured sausages with different water
addition levels
Different superscript letters represent significant differences
between results (p<0.05).
CIE, International Commission on Illumination.
Data are presented as mean±SD.WAL0, 100% pork; WAL10, 90% pork/10% water;
WAL20, 80% pork/20% water; WAL30, 70%
pork/30% water; WAL40, 60% pork/40% water;
WAL50, 50% pork/50% water of semi-dried restructured
sausage.Different superscript letters represent significant differences
between results (p<0.05).CIE, International Commission on Illumination.Color of semi-dried restructured sausages are presented as the L*, a*, and b*
values in Table 3. The significant
increase of L* and b* values were observed in the WAL40 and WAL50 group than
WAL0 group, and increment of water addition levels induced the decrease of a*
values (p<0.05). The increase in the L* values of sausages with the
addition of water may result from the shortened drying time. During the drying
process, Maillard browning reactions occur, which are associated with the darker
color of the products (Akonor et al.,
2016). A previous study showed that increased drying time induced lower
L* and b* values in restructured jerky (Kim et
al., 2021b). Taken together, our results show that the shortened
drying time owing to the addition of water to the semi-dried restructured
sausage blocked the excessive browning reactions.
Water activity of semi-dried restructured sausages
The water activity of the semi-dried restructured sausages gradually decreased
depending on the drying time (Fig. 2). As
the amount of added water increased, the water activity of the sausages
decreased markedly. Previous studies have reported that thermal conductivity
increased with an increase in the moisture content of foods (Modi et al., 2014). In addition, our data
show that the addition of water increased the drying rate (Fig. 1). Thus, the decrease in water activity with the
addition of water to the sausage resulted from the increase in thermal
conductivity, which accelerates the decrease in free water. Moreover, water
activity is one of the most crucial factors related to the classification
criteria for semi-dried restructured sausages (Jang et al., 2015), and the safety of microorganisms in semi-dried
foods. Water activity between 0.60−0.90 is desirable for intermediate
moisture foods (Aguilera and
Gutiérrez-López, 2018). In our study, with water
addition levels of 0%, 10%, 20%, 30%, 40%,
and 50% of sausages, the time required for the water activities of
sausages to reach below 0.90 were 300, 240, 180, 180, 180, and 180 min and the
time to reach water activities below 0.80 were 580, 360, 360, 360, 300, and 300
min, respectively. Our data indicate that the addition of water to semi-dried
restructured sausages positively influences microbiological safety by decreasing
water activity.
Fig. 2.
Water activity of semi-dried restructured sausages with different
water addition levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (10, 20, 30, 40,
50, 60, 80, 100, 120, 150, 180, 240, 300, 360, and 580 min). The error
bars indicate SD. a–f Different superscript letters
represent significant differences at the drying time
(p<0.05).
Water activity of semi-dried restructured sausages with different
water addition levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (10, 20, 30, 40,
50, 60, 80, 100, 120, 150, 180, 240, 300, 360, and 580 min). The error
bars indicate SD. a–f Different superscript letters
represent significant differences at the drying time
(p<0.05).
Porosity, shear force, and VBN of semi-dried restructured sausages
The porosity of semi-dried restructured sausages was significantly affected by
the water addition levels in the samples (p<0.05) (Table 4), and the WAL50 group had the highest porosity and
WAL20, WAL30, and WAL40 groups had significantly higher porosity than WAL0 group
(p<0.05). This result could be explained by the increased number of water
molecules, which might induce the formation of porous structures in the samples
during the drying process (Kim et al.,
2022). Different water contents in food materials affect the size
and/or number of pores (Wang and Liapis,
2012). In addition, the high moisture content and porous structure of
food materials shorten the drying time, as they accelerate heat and mass
transfer during the drying process (Kim et al.,
2022). Therefore, the high porosity from the increased amount of
added water in the samples could explain the drying rate of the semi-dried
restructured sausages.
Table 4.
Porosity and shear force of semi-dried restructured sausages with
different water addition levels
Different superscript letters represent significant differences
(p<0.05).
Data are presented as mean±SD.WAL0, 100% pork; WAL10, 90% pork/10% water;
WAL20, 80% pork/20% water; WAL30, 70%
pork/30% water; WAL40, 60% pork/40% water;
WAL50, 50% pork/50% water of semi-dried restructured
sausage.Different superscript letters represent significant differences
(p<0.05).An extended drying time causes excessive hardening in meat products, and the
shear force is associated with the tenderness of meat products (Shi et al., 2021a). In the present study,
the shear force of the sausages was significantly affected by the moisture
content of the samples (p<0.05) (Table
4). The shear force of the sausages significantly decreased with
increasing initial water content, except WAL10 group (p<0.05). These
results can be explained by the reduced drying time of samples with added water.
During drying, myofibrillar protein is denatured by heat and extended drying
time, which causes shrinkage of myofibrillar protein (Shi et al., 2021a). This shrinkage induces tough texture of
dried meat products, and the excessive hardness of dried meat negatively
influences consumer preference (Kim et al.,
2010). Collectively, our data suggest that increased moisture content
leads to lower shear forces in semi-dried restructured sausages resulting from
the increased formation of porous structures during the drying process.The VBN values of the semi-dried restructured sausages are shown in Fig. 3. The increment of water addition
levels in the sausages decreased the VBN values (p<0.05), although WAL10
group did not showed significant difference to WAL0 group (p>0.05). In
the case of dried food, VBN values increase as the drying progresses because of
the generation of volatile nitrogen compounds (Chen et al., 2004). Previous study reported that extended drying
time significantly increases VBN values of dried yellow corvina (Gwak and Eun, 2010). Thus, the lower VBN
values observed in the groups with more than 20% of moisture addition
levels might result from the shortened drying time.
Fig. 3.
Volatile basic nitrogen (VBN) values of semi-dried restructured
sausages produced with different water addition levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (360, 350, 350,
300, 270, and 270 min), which were determined for the same condition of
below 0.5 g/g moisture content on the dry basis. a–d
Different superscript letters represent significant differences of the
results (p<0.05). VBN values are expressed as mg%.
Volatile basic nitrogen (VBN) values of semi-dried restructured
sausages produced with different water addition levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (360, 350, 350,
300, 270, and 270 min), which were determined for the same condition of
below 0.5 g/g moisture content on the dry basis. a–d
Different superscript letters represent significant differences of the
results (p<0.05). VBN values are expressed as mg%.
Microstructure of semi-dried restructured sausages
The microstructures of the semi-dried restructured sausages with different
initial moisture contents were observed using FE-SEM (Fig. 4). The addition of water to the sausages formed pores,
and the size and number of pores were markedly changed with the increase in
initial moisture content. In WAL0 to WAL20 group, increase of water addition
levels in semi-dried restructured sausages induced the increased number of small
pores. In WAL30 to WAL50 group, the addition of water expanded the size of
pores. During the drying process of meat products, heat treatment induces
denaturation of myosin and decreases the water-binding capacity through the
migration of water from the interior to the surface, resulting in the shrinkage
of meat (Shi et al., 2021a). In this
progress, water molecules were evaporated and formed a number of pores. The
larger pore size was influenced by the increased rate of moisture migration
(Labuza and Hyman, 1998). As observed
in our data, the addition of water positively affected the porosity and pore
size of the semi-dried restructured sausage by shortening the drying time.
Indeed, the acceleration of moisture loss induces a higher number and size of
pores in the semi-dried restructured jerky during the drying process (Kim et al., 2021b). Collectively, our data
suggest that increasing the initial moisture content leads to a more porous
structure, which improves the drying characteristics and physicochemical
properties of semi-dried restructured sausages.
Fig. 4.
Microstructure of semi-dried restructured sausages produced with
different water addition levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (360, 350, 350,
300, 270, and 270 min), which were determined for the same condition of
below 0.5 g/g moisture content on the dry basis. Microstructure of
semi-dried restructure sausages was observed using field emission
scanning electron microscopy. Magnification of all images was
×300.
Microstructure of semi-dried restructured sausages produced with
different water addition levels.
WAL0, 100% pork; WAL10, 90% pork/10% water; WAL20,
80% pork/20% water; WAL30, 70% pork/30%
water; WAL40, 60% pork/40% water; WAL50, 50%
pork/50% water of semi-dried restructured sausage. The samples
were dried at 85°C for different time periods (360, 350, 350,
300, 270, and 270 min), which were determined for the same condition of
below 0.5 g/g moisture content on the dry basis. Microstructure of
semi-dried restructure sausages was observed using field emission
scanning electron microscopy. Magnification of all images was
×300.
Conclusion
Our results demonstrated that increased initial moisture content in semi-dried
restructured sausages shortened the drying time and increased thermal diffusivity.
Particularly, WAL20, WAL30, WAL40, and WAL50 showed significantly improved
physicochemical properties, including porosity, shear force, and VBN, compared with
those of WAL0 (p<0.05). The structural difference in semi-dried restructured
sausages was observed where the increment of initial moisture content resulted in
improved drying characteristics. In conclusion, our data suggest that an increase in
initial moisture content, especially more than 20% (w/w), is a good method
for improving the drying characteristics and physicochemical properties of
semi-dried restructured sausages.