Literature DB >> 35575890

Genotyping of African Swine Fever Virus.

Paulina Rajko-Nenow1, Carrie Batten2.   

Abstract

Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral diseases of livestock. Real-time PCR (qPCR) is known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, followed by Sanger sequencing and data analysis. Here, we describe the ASFV genotyping method used at the OIE Reference Laboratory for ASF at the Pirbright Institute, United Kingdom.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Gel electrophoresis; Genotyping; Polymerase chain reaction; Sequencing

Mesh：

Substances：

Year:  2022        PMID： 35575890     DOI： 10.1007/978-1-0716-2333-6_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Building phylogenetic trees from molecular data with MEGA.

Authors:  Barry G Hall
Journal:  Mol Biol Evol       Date:  2013-03-13       Impact factor: 16.240

Review 2.  African swine fever virus replication and genomics.

Authors:  Linda K Dixon; David A G Chapman; Christopher L Netherton; Chris Upton
Journal:  Virus Res       Date:  2012-11-08       Impact factor: 3.303

3.  Evolution of African swine fever virus genes related to evasion of host immune response.

Authors:  Magdalena Frączyk; Grzegorz Woźniakowski; Andrzej Kowalczyk; Łukasz Bocian; Edyta Kozak; Krzysztof Niemczuk; Zygmunt Pejsak
Journal:  Vet Microbiol       Date:  2016-08-20       Impact factor: 3.293

4.  Improved Strategy for Molecular Characterization of African Swine Fever Viruses from Sardinia, Based on Analysis of p30, CD2V and I73R/I329L Variable Regions.

Authors:  G Sanna; S Dei Giudici; D Bacciu; P P Angioi; M Giammarioli; G M De Mia; A Oggiano
Journal:  Transbound Emerg Dis       Date:  2016-05-13       Impact factor: 5.005

5.  Genotyping field strains of African swine fever virus by partial p72 gene characterisation.

Authors:  A D S Bastos; M-L Penrith; C Crucière; J L Edrich; G Hutchings; F Roger; E Couacy-Hymann; G R Thomson
Journal:  Arch Virol       Date:  2003-04       Impact factor: 2.574

6.  Detection of African swine fever virus in the tissues of asymptomatic pigs in smallholder farming systems along the Kenya-Uganda border: implications for transmission in endemic areas and ASF surveillance in East Africa.

Authors:  Edward Okoth Abworo; Cynthia Onzere; Joshua Oluoch Amimo; Victor Riitho; Waithaka Mwangi; Jocelyn Davies; Sandra Blome; Richard Peter Bishop
Journal:  J Gen Virol       Date:  2017-07-10       Impact factor: 3.891

7.  Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes.

Authors:  Carmina Gallardo; Dufton M Mwaengo; Joseph M Macharia; Marisa Arias; Evans A Taracha; Alejandro Soler; Edward Okoth; Elena Martín; Jackline Kasiti; Richard P Bishop
Journal:  Virus Genes       Date:  2008-11-14       Impact factor: 2.332

8.  Genetic variation among African swine fever genotype II viruses, eastern and central Europe.

Authors:  Carmina Gallardo; Jovita Fernández-Pinero; Virginia Pelayo; Ismail Gazaev; Iwona Markowska-Daniel; Gediminas Pridotkas; Raquel Nieto; Paloma Fernández-Pacheco; Svetlana Bokhan; Oleg Nevolko; Zhanna Drozhzhe; Covadonga Pérez; Alejandro Soler; Denis Kolvasov; Marisa Arias
Journal:  Emerg Infect Dis       Date:  2014-09       Impact factor: 6.883

9.  Comparative Analysis of Whole-Genome Sequence of African Swine Fever Virus Belgium 2018/1.

Authors:  Jan H Forth; Marylène Tignon; Ann Brigitte Cay; Leonie F Forth; Dirk Höper; Sandra Blome; Martin Beer
Journal:  Emerg Infect Dis       Date:  2019-06-17       Impact factor: 6.883

10.  Phylogeographic Analysis of African Swine Fever Virus, Western Europe, 2018.

Authors:  Mutien Garigliany; Daniel Desmecht; Marylène Tignon; Dominique Cassart; Christophe Lesenfant; Julien Paternostre; Rosario Volpe; Ann Brigitte Cay; Thierry van den Berg; Annick Linden
Journal:  Emerg Infect Dis       Date:  2019-01-17       Impact factor: 6.883
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.