Literature DB >> 35575886

Primary Macrophage Culture from Porcine Blood and Lungs.

Lynnette C Goatley1, Rachel Nash2, Christopher L Netherton3.   

Abstract

Primary cultures represent the most reliable method to isolate and propagate field isolates of African swine fever virus (ASFV ). Within the pig ASFV predominantly targets the reticuloendothelial system for replication; therefore, primary macrophage cell cultures are commonly used to isolate, propagate, and study the virus life cycle in the laboratory. In this chapter we will describe methods for the direct isolation of pulmonary alveolar macrophages by lung lavage and the culture of monocyte-derived macrophages from pig blood. We also include a method for the positive selection of CD14+ monocytes as a source for monocyte-derived macrophages from pig blood using microbeads.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  African swine fever; Buffy coat; Macrophage; Monocyte; Peripheral blood mononuclear cells; Virus

Mesh：

Year:  2022        PMID： 35575886     DOI： 10.1007/978-1-0716-2333-6_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  PROPAGATION AND MODIFICATION OF AFRICAN SWINE FEVER VIRUS IN CELL CULTURES.

Authors:  W R HESS; B F COX; W P HEUSCHELE; S S STONE
Journal:  Am J Vet Res       Date:  1965-01       Impact factor: 1.156

2.  Hemadsorption and cytopathic effect produced by African Swine Fever virus in swine bone marrow and buffy coat cultures.

Authors:  W A MALMQUIST; D HAY
Journal:  Am J Vet Res       Date:  1960-01       Impact factor: 1.156

3.  Titration of African swine fever (ASF) virus.

Authors:  L Enjuanes; A L Carrascosa; M A Moreno; E Viñuela
Journal:  J Gen Virol       Date:  1976-09       Impact factor: 3.891

4.  The progressive adaptation of a georgian isolate of African swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major modifications of the viral genome.

Authors:  Peter W Krug; Lauren G Holinka; Vivian O'Donnell; Bo Reese; Brenton Sanford; Ignacio Fernandez-Sainz; Douglas P Gladue; Jonathan Arzt; Luis Rodriguez; Guillermo R Risatti; Manuel V Borca
Journal:  J Virol       Date:  2014-12-10       Impact factor: 5.103

5.  A Cell Culture-Adapted Vaccine Virus against the Current African Swine Fever Virus Pandemic Strain.

Authors:  M V Borca; A Rai; E Ramirez-Medina; E Silva; L Velazquez-Salinas; E Vuono; S Pruitt; N Espinoza; D P Gladue
Journal:  J Virol       Date:  2021-06-24       Impact factor: 5.103

6.  An immortalized porcine macrophage cell line competent for the isolation of African swine fever virus.

Authors:  Kentaro Masujin; Tomoya Kitamura; Ken -Ichiro Kameyama; Kota Okadera; Tatsuya Nishi; Takato Takenouchi; Hiroshi Kitani; Takehiro Kokuho
Journal:  Sci Rep       Date:  2021-02-26       Impact factor: 4.379

7.  A porcine macrophage cell line that supports high levels of replication of OURT88/3, an attenuated strain of African swine fever virus.

Authors:  Raquel Portugal; Lynnette C Goatley; Robert Husmann; Federico A Zuckermann; Linda K Dixon
Journal:  Emerg Microbes Infect       Date:  2020-12       Impact factor: 7.163
  7 in total

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