| Literature DB >> 35554910 |
Kai Sen Tan1, Akshamal M Gamage2, Jing Liu3.
Abstract
The nasal epithelium lining the human upper airway is the primary portal of entry for several respiratory pathogens, including the recently emerged SARS-CoV-2 virus responsible for the ongoing COVID-19 pandemic. Here, we describe in detail methods for in vitro ALI differentiation of primary cells collected from human donors, to obtain differentiated hNECs. This can serve as a physiologically relevant model to investigate various aspects of host-pathogen responses to SARS-CoV-2 and other emerging respiratory viruses.Entities:
Keywords: Air–liquid interface; Host–pathogen interaction; Human nasal epithelial cells; SARS-CoV-2; Viral kinetics
Mesh：
Year: 2022 PMID： 35554910 DOI： 10.1007/978-1-0716-2111-0_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745