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Literature DB >> 35524129

Bulk-Fitness Measurements Using Barcode Sequencing Analysis in Yeast.

Claire A Chochinov^1,2, Alex N Nguyen Ba^3,4.

Abstract

The use of DNA barcodes for determining changes in genotype frequencies has been instrumental to increase the scale at which we can phenotype strain libraries by using next-generation sequencing technologies. Here, we describe the determination of strain fitness for thousands of yeast strains simultaneously in a single assay using recent innovations that increase the precision of these measurements, such as the inclusion of unique-molecular identifiers (UMIs) and purification by solid-phase reverse immobilization (SPRI) beads.

Entities: Chemical

Keywords: Bulk fitness assays; DNA barcodes; Strain fitness determination; Unique molecular identifiers

Mesh：

Substances：
DNA

Year: 2022 PMID： 35524129 DOI： 10.1007/978-1-0716-2257-5_22

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Bulk-Fitness Measurements Using Barcode Sequencing Analysis in Yeast.

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