Chunge Cui1, Qiumei Lu1, Zhangchen Zhao1, Shan Lu1, Shan Duan1, Yang Yang1, Yue Qiao1, Liang Chen2, Yin-Gang Hu3,4. 1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China. 2. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China. chenliang9117@nwsuaf.edu.cn. 3. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, China. huyingang@nwsuaf.edu.cn. 4. Institute of Water Saving Agriculture in Arid Regions of China, Northwest A&F University, Yangling, Shaanxi, China. huyingang@nwsuaf.edu.cn.
Abstract
MAIN CONCLUSION: Rht5 was narrowed to an approximately 1 Mb interval and had pleiotropic effects on plant height, spike length and grain size. TraesCS3B02G025600 was predicted as the possible candidate gene. Plant height is an important component related to plant architecture, lodging resistance, and yield performance. The utilization of dwarf genes has made great contributions to wheat breeding and production. In this study, two F2 populations derived from the crosses of Jinmai47 and Ningchun45 with Marfed M were employed to identify the genetic region of reduce plant height 5 (Rht5), and their derived lines were used to evaluate its effects on plant height and main agronomic traits. Rht5 was fine-mapped between markers Kasp-25 and Kasp-23, in approximately 1 Mb region on chromosome 3BS, which harbored 17 high-confidence annotated genes based on the reference genome of Chinese Spring (IWGSC RefSeq v1.1). TraesCS3B02G025600 were predicted as the possible candidate gene based on its differential expression and sequence variation between dwarf and tall lines and parents. The results of phenotypic evaluation showed that Rht5 had pleiotropic effects on plant height, spike length, culm diameter, grain size and grain yield. The plant height of Rht5 dwarf lines was reduced by an average of 32.67% (32.53 cm) and 27.84% (33.62 cm) in the Jinmai47 and Ningchun45 population, respectively. While Rht5 showed significant and negative pleiotropic effects on culm diameter, aboveground biomass, grain yield, spike length, spikelet number, grain number per spike, grain size, grain weight and filling degree of basal second internode. The culm lodging resistance index (CLRI) of dwarf lines was significantly higher than that of tall lines in the two population. In conclusion, these results lay a foundation for understanding the dwarfing mechanism of Rht5.
MAIN CONCLUSION: Rht5 was narrowed to an approximately 1 Mb interval and had pleiotropic effects on plant height, spike length and grain size. TraesCS3B02G025600 was predicted as the possible candidate gene. Plant height is an important component related to plant architecture, lodging resistance, and yield performance. The utilization of dwarf genes has made great contributions to wheat breeding and production. In this study, two F2 populations derived from the crosses of Jinmai47 and Ningchun45 with Marfed M were employed to identify the genetic region of reduce plant height 5 (Rht5), and their derived lines were used to evaluate its effects on plant height and main agronomic traits. Rht5 was fine-mapped between markers Kasp-25 and Kasp-23, in approximately 1 Mb region on chromosome 3BS, which harbored 17 high-confidence annotated genes based on the reference genome of Chinese Spring (IWGSC RefSeq v1.1). TraesCS3B02G025600 were predicted as the possible candidate gene based on its differential expression and sequence variation between dwarf and tall lines and parents. The results of phenotypic evaluation showed that Rht5 had pleiotropic effects on plant height, spike length, culm diameter, grain size and grain yield. The plant height of Rht5 dwarf lines was reduced by an average of 32.67% (32.53 cm) and 27.84% (33.62 cm) in the Jinmai47 and Ningchun45 population, respectively. While Rht5 showed significant and negative pleiotropic effects on culm diameter, aboveground biomass, grain yield, spike length, spikelet number, grain number per spike, grain size, grain weight and filling degree of basal second internode. The culm lodging resistance index (CLRI) of dwarf lines was significantly higher than that of tall lines in the two population. In conclusion, these results lay a foundation for understanding the dwarfing mechanism of Rht5.
Authors: Eduard D Akhunov; Andrew W Goodyear; Shu Geng; Li-Li Qi; Benjamin Echalier; Bikram S Gill; J Perry Gustafson; Gerard Lazo; Shiaoman Chao; Olin D Anderson; Anna M Linkiewicz; Jorge Dubcovsky; Mauricio La Rota; Mark E Sorrells; Deshui Zhang; Henry T Nguyen; Venugopal Kalavacharla; Khwaja Hossain; Shahryar F Kianian; Junhua Peng; Nora L V Lapitan; Jose L Gonzalez-Hernandez; James A Anderson; Dong-Woog Choi; Timothy J Close; Muharrem Dilbirligi; Kulvinder S Gill; M Kay Walker-Simmons; Camille Steber; Patrick E McGuire; Calvin O Qualset; Jan Dvorak Journal: Genome Res Date: 2003-04-14 Impact factor: 9.043
Authors: Maria Buerstmayr; Barbara Steiner; Christian Wagner; Petra Schwarz; Klaus Brugger; Delfina Barabaschi; Andrea Volante; Giampiero Valè; Luigi Cattivelli; Hermann Buerstmayr Journal: Plant Biotechnol J Date: 2017-11-10 Impact factor: 9.803