| Literature DB >> 35478224 |
Nelly M Cruz1,2,3,4, Raghava Reddy1,2,3,4, José L McFaline-Figueroa5, Christine Tran1,2,3,4, Hongxia Fu3,4,6,7, Benjamin S Freedman8,9,10,11,12,13.
Abstract
The functions of cilia-antenna-like organelles associated with a spectrum of disease states-are poorly understood, particularly in human cells. Here we show that human pluripotent stem cells (hPSCs) edited via CRISPR to knock out the kinesin-2 subunits KIF3A or KIF3B can be used to model ciliopathy phenotypes and to reveal ciliary functions at the tissue scale. KIF3A-/- and KIF3B-/- hPSCs lacked cilia, yet remained robustly self-renewing and pluripotent. Tissues and organoids derived from these hPSCs displayed phenotypes that recapitulated defective neurogenesis and nephrogenesis, polycystic kidney disease (PKD) and other features of the ciliopathy spectrum. We also show that human cilia mediate a critical switch in hedgehog signalling during organoid differentiation, and that they constitutively release extracellular vesicles containing signalling molecules associated with ciliopathy phenotypes. The capacity of KIF3A-/- and KIF3B-/- hPSCs to reveal endogenous mechanisms underlying complex ciliary phenotypes may facilitate the discovery of candidate therapeutics.Entities:
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Year: 2022 PMID： 35478224 PMCID： PMC9228023 DOI： 10.1038/s41551-022-00880-8
Source DB: PubMed Journal: Nat Biomed Eng ISSN： 2157-846X Impact factor: 29.234