Serdar Dogan1, Gezmis Kimyon2, Huseyin Ozkan3, Filiz Kacmaz4, Baran Camdeviren4, Irem Karaaslan5. 1. Department of Biochemistry, Faculty of Medicine, Hatay Mustafa Kemal University, Antakya, Hatay, 31060, Turkey. drserdardogan@hotmail.com. 2. Department of Rheumatology, Faculty of Medicine, Hatay Mustafa Kemal University, Antakya, Hatay, 31060, Turkey. 3. Department of Genetics, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Antakya, Hatay, 31060, Turkey. 4. Department of Molecular Biochemistry and Genetics, Health Science Institute, Hatay Mustafa Kemal University, Antakya, Hatay, 31060, Turkey. 5. Research and Application Center for Technology and Research and Development, Hatay Mustafa Kemal University, Antakya, Hatay, 31060, Turkey.
Abstract
OBJECTIVE: The present study aimed to examine the effects of cDMARD and bDMARD therapy on both gene expressions and protein levels of TNF-α, IL-6, IL-10 and fatty acid levels in patients with RA. METHOD: Plasma TNF-α, IL-6, and IL-10 levels were examined by the ELISA method, while TNF-α, IL-6, and IL-10 gene expression levels were examined by RT-qPCR, and fatty acid levels were examined by GC/MS. RESULTS: IL-10 gene expression levels significantly increased in RA patients receiving cDMARD treatment compared to those of the control group. Also, eicosadienoic acid, myristoleic acid and capric acid levels were significantly lower in the patient groups compared to those in the control group. CONCLUSION: The drugs used in the treatment of RA had no effect on the fatty acid levels whereas had effects on the mRNA and protein levels of the target cytokines.
OBJECTIVE: The present study aimed to examine the effects of cDMARD and bDMARD therapy on both gene expressions and protein levels of TNF-α, IL-6, IL-10 and fatty acid levels in patients with RA. METHOD: Plasma TNF-α, IL-6, and IL-10 levels were examined by the ELISA method, while TNF-α, IL-6, and IL-10 gene expression levels were examined by RT-qPCR, and fatty acid levels were examined by GC/MS. RESULTS: IL-10 gene expression levels significantly increased in RA patients receiving cDMARD treatment compared to those of the control group. Also, eicosadienoic acid, myristoleic acid and capric acid levels were significantly lower in the patient groups compared to those in the control group. CONCLUSION: The drugs used in the treatment of RA had no effect on the fatty acid levels whereas had effects on the mRNA and protein levels of the target cytokines.
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