Alyusif Ameen1, Sasidharanpillai Sabeena2, Sudandiradas Robin3, Ramachandran Sanjay4, Varamballi Prasad5, Fernandes M Mevis6, Puneet Bhatt7, Govindakarnavar Arunkumar8. 1. Research Assistant (Clinical Virology), Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India. 2. Assistant Professor, Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India. 3. Additional Project Administrator, Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India. 4. Biotechnologist, Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India. 5. Senior Data Analyst, Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India. 6. Research Staff (Clinical Virology), Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India. 7. Research Scholar, Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India. 8. Director, Manipal Institute of Virology, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, India.
Abstract
Background: This study was carried out to understand the circulating genotypes of Hepatitis A virus (HAV) in South West, East and North East India during the period 2017-2018 as a part of acute febrile illness surveillance at the Manipal Institute of Virology. Methods: Archived serum samples of 48 Hepatitis A confirmed cases were subjected to RNA extraction using QIAamp® Viral RNA Mini Kit (QIAGEN, Germany). The samples with molecular confirmation for HAV by reverse transcriptase real-Time PCR (Real Star® HAV RT-PCR Kit 2.0, Altona Diagnostics, GmbH, Hamburg, Germany) were further subjected to nested conventional PCR targeting the 5' UTR region. The purified PCR products were sequenced using Big Dye Terminator Kit (Applied Biosystems, USA), in a 3500 XL genetic analyzer (Applied Biosystems, USA). The edited sequences by means of MEGA X (MEGA version 10.1) were compared with reference sequences in the NCBI nucleotide database. Results: From states of Assam, Goa, Gujarat, Karnataka, Kerala, Maharashtra, Odisha, Tamil Nadu and Tripura, 139 Hepatitis A and 33 Hepatitis E cases were reported during the study period. The median age of the acute Hepatitis A cases was 19 years (IQR 12.8-24) and most of the affected individuals were students between 10 and 19 years (52.5%). In the present study, 14 samples from Assam, Goa, Gujarat, Karnataka, Odisha, Kerala, Maharashtra and Tamil Nadu were genotyped as genotype IIIA by nested conventional polymerase chain reaction. Conclusion: The circulating HAV genotype in South West, North East and East India between 2017 and 2018 was IIIA.
Background: This study was carried out to understand the circulating genotypes of Hepatitis A virus (HAV) in South West, East and North East India during the period 2017-2018 as a part of acute febrile illness surveillance at the Manipal Institute of Virology. Methods: Archived serum samples of 48 Hepatitis A confirmed cases were subjected to RNA extraction using QIAamp® Viral RNA Mini Kit (QIAGEN, Germany). The samples with molecular confirmation for HAV by reverse transcriptase real-Time PCR (Real Star® HAV RT-PCR Kit 2.0, Altona Diagnostics, GmbH, Hamburg, Germany) were further subjected to nested conventional PCR targeting the 5' UTR region. The purified PCR products were sequenced using Big Dye Terminator Kit (Applied Biosystems, USA), in a 3500 XL genetic analyzer (Applied Biosystems, USA). The edited sequences by means of MEGA X (MEGA version 10.1) were compared with reference sequences in the NCBI nucleotide database. Results: From states of Assam, Goa, Gujarat, Karnataka, Kerala, Maharashtra, Odisha, Tamil Nadu and Tripura, 139 Hepatitis A and 33 Hepatitis E cases were reported during the study period. The median age of the acute Hepatitis A cases was 19 years (IQR 12.8-24) and most of the affected individuals were students between 10 and 19 years (52.5%). In the present study, 14 samples from Assam, Goa, Gujarat, Karnataka, Odisha, Kerala, Maharashtra and Tamil Nadu were genotyped as genotype IIIA by nested conventional polymerase chain reaction. Conclusion: The circulating HAV genotype in South West, North East and East India between 2017 and 2018 was IIIA.
Authors: G De Serres; T L Cromeans; B Levesque; N Brassard; C Barthe; M Dionne; H Prud'homme; D Paradis; C N Shapiro; O V Nainan; H S Margolis Journal: J Infect Dis Date: 1999-01 Impact factor: 5.226
Authors: Bharti Malhotra; Anu Kanwar; P V Janardhan Reddy; Aradhana Chauhan; Jitendra Tiwari; Shipra Bhargava; H N Verma Journal: Indian J Med Res Date: 2018-05 Impact factor: 2.375