A Comparative Analysis for the Production of Xylooligosaccharides via Enzymatic Hydrolysis from Sugarcane Bagasse and Coconut Coir.

Xylooligosaccharides are known prebiotics that various foods and feed industries can utilize. In the present study, the xylan was extracted from sugarcane bagasse and coconut coir by NaOH treatment and it was further structurally characterized by FTIR. Furthermore, Thermomyces lanuginosus VAPS-24 was used to produce endo-xylanase, which induced the production of XOS from both of these substrates. The maximum production of XOS in 8 h using 20U enzyme concentration and 2% substrate concentration was found as 10.10 mg/ml, 5.43 mg/ml for sugarcane bagasse and coconut coir, respectively. Further, the produced XOS was structurally characterized by the NMR, HPLC and FTIR analysis. This study gives a clue that the sugarcane bagasse can be used as the potent producer for the xylooligosaccharides as compared to the coconut coir and can be utilized at the industrial level. Supplementary Information: The online version contains supplementary material available at 10.1007/s12088-022-01010-3. © Association of Microbiologists of India 2022.