Literature DB >> 3545892

A practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity.

L W Weber.   

Abstract

A known ornithine decarboxylase assay working with ion exchange separation of [3H]ornithine and [3H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles [3H]putrescine formed.

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Year:  1987        PMID： 3545892     DOI： 10.1007/BF01942841

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  11 in total

1.  Apparent kinetic differences between brain and liver ornithine decarboxylase.

Authors:  S R Butler; S M Schanberg
Journal:  Life Sci       Date:  1976-04-01       Impact factor: 5.037

2.  Ornithine decarboxylase assay using ion-exchange paper.

Authors:  J L Clark
Journal:  Anal Biochem       Date:  1976-08       Impact factor: 3.365

3.  Ornithine decarboxylase activity in developing rat brain.

Authors:  T R Anderson; S M Schanberg
Journal:  J Neurochem       Date:  1972-06       Impact factor: 5.372

4.  A biosynthetic ornithine decarboxylase in Escherichia coli.

Authors:  D R Morris; A B Pardee
Journal:  Biochem Biophys Res Commun       Date:  1965-09-22       Impact factor: 3.575

5.  A radiometric microassay for ornithine decarboxylase.

Authors:  J L Maderdrut; R W Oppenheim
Journal:  Neuroscience       Date:  1978       Impact factor: 3.590

6.  Regulation of brain ornithine decarboxylase activity in the neonatal rat.

Authors:  G Morris; C Lau; T A Slotkin
Journal:  Eur J Pharmacol       Date:  1983-03-25       Impact factor: 4.432

7.  Ornithine decarboxylase (EC 4.1.1.17) assay based upon the retention of putrescine by a strong cation-exchange paper.

Authors:  R Djurhuus
Journal:  Anal Biochem       Date:  1981-05-15       Impact factor: 3.365

8.  Pyridoxal 5'-phosphate and the regulation of ornithine decarboxylase activity and stability.

Authors:  J L Clark; J L Fuller
Journal:  Eur J Biochem       Date:  1976-08-01

9.  Developmental changes in mouse brain polyamine metabolism.

Authors:  S I Laitinen; P H Laitinen; O A Hietala; A E Pajunen; R S Piha
Journal:  Neurochem Res       Date:  1982-12       Impact factor: 3.996

10.  Purification and properties of ornithine decarboxylase from rat liver.

Authors:  T Kitani; H Fujisawa
Journal:  J Biol Chem       Date:  1983-01-10       Impact factor: 5.157
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  2 in total

1.  Hormonal and enzymatic parameters of hepatic regeneration in patients undergoing major liver resections.

Authors:  A Francavilla; C Panella; L Polimeno; A Giangaspero; V Mazzaferro; C E Pan; D H Van Thiel; T E Starzl
Journal:  Hepatology       Date:  1990-11       Impact factor: 17.425

2.  Relationship between plasma and hepatic cytosolic levels of ornithine decarboxylase (ODC) and thymidine kinase (TK) in 70% hepatectomized rats.

Authors:  L Polimeno; A Azzarone; P Dell'Aquila; C Amoruso; M Barone; A Angelini; D H Van Thiel; A Francavilla
Journal:  Dig Dis Sci       Date:  1991-03       Impact factor: 3.199
  2 in total

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