Literature DB >> 3542565

Role of tryptophan 54 in the binding of E. coli single-stranded DNA-binding protein to single-stranded polynucleotides.

M I Khamis, J R Casas-Finet, A H Maki, J B Murphy, J W Chase.   

Abstract

Fluorescence and optical detection of triplet state magnetic resonance spectroscopy have been employed to study the complexes formed by single-stranded polynucleotides with both E. coli single-stranded DNA-binding protein and an E. coli ssb gene product in which Trp-54 is replaced by phenylalanine using site specific oligonucleotide mutagenesis. Our results strongly suggest the involvement of Trp-54 in stabilizing the protein-nucleic acid complexes via stacking interactions of the aromatic residue with the nucleotide bases.

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Year:  1987        PMID： 3542565     DOI： 10.1016/0014-5793(87)81427-8

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  12 in total

1.  Structural Mechanisms of Cooperative DNA Binding by Bacterial Single-Stranded DNA-Binding Proteins.

Authors:  Katarzyna Dubiel; Angela R Myers; Alexander G Kozlov; Olivia Yang; Jichuan Zhang; Taekjip Ha; Timothy M Lohman; James L Keck
Journal:  J Mol Biol       Date:  2018-11-22       Impact factor: 5.469

2.  Is a fully wrapped SSB-DNA complex essential for Escherichia coli survival?

Authors:  Vincent M Waldman; Elizabeth Weiland; Alexander G Kozlov; Timothy M Lohman
Journal:  Nucleic Acids Res       Date:  2016-04-15       Impact factor: 16.971

3.  Triplet state sublevel kinetics of tryptophan 54 in the complex of Escherichia coli single-stranded DNA binding protein with single-stranded poly(deoxythymidylic) acid.

Authors:  L H Zang; A H Maki; J B Murphy; J W Chase
Journal:  Biophys J       Date:  1987-11       Impact factor: 4.033

Review 4.  The single-stranded DNA-binding protein of Escherichia coli.

Authors:  R R Meyer; P S Laine
Journal:  Microbiol Rev       Date:  1990-12

5.  A stable tetramer is not the only oligomeric state that mitochondrial single-stranded DNA binding proteins can adopt.

Authors:  Saurabh P Singh; Vandna Kukshal; Roberto Galletto
Journal:  J Biol Chem       Date:  2019-01-07       Impact factor: 5.157

6.  Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).

Authors:  Y S Wang; J D Hall
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

7.  Dynamic stepwise opening of integron attC DNA hairpins by SSB prevents toxicity and ensures functionality.

Authors:  Maj Svea Grieb; Aleksandra Nivina; Bevan L Cheeseman; Andreas Hartmann; Didier Mazel; Michael Schlierf
Journal:  Nucleic Acids Res       Date:  2017-10-13       Impact factor: 16.971

8.  Triplet state properties of tryptophan residues in complexes of mutated Escherichia coli single-stranded DNA binding proteins with single-stranded polynucleotides.

Authors:  D H Tsao; J R Casas-Finet; A H Maki; J W Chase
Journal:  Biophys J       Date:  1989-05       Impact factor: 4.033

Review 9.  Human single-stranded DNA binding proteins are essential for maintaining genomic stability.

Authors:  Nicholas W Ashton; Emma Bolderson; Liza Cubeddu; Kenneth J O'Byrne; Derek J Richard
Journal:  BMC Mol Biol       Date:  2013-04-01       Impact factor: 2.946

10.  Physical and functional interactions between human mitochondrial single-stranded DNA-binding protein and tumour suppressor p53.

Authors:  Tuck Seng Wong; Sridharan Rajagopalan; Fiona M Townsley; Stefan M Freund; Miriana Petrovich; David Loakes; Alan R Fersht
Journal:  Nucleic Acids Res       Date:  2008-12-09       Impact factor: 16.971
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