Literature DB >> 3542240

A model for in vitro proliferation of undifferentiated bovine mammary epithelial cells.

A Shamay, A Gertler.   

Abstract

A method for culturing bovine undifferentiated mammary epithelial cells embedded in collagen gels is described. Cell growth was quantitated by incorporation of tritiated thymidine. Maximal rate of incorporation was achieved in media supplemented with sera, however considerable growth was observed in serum-free medium supplemented with insulin, transferrin and selenium. The growth promoting effect of insulin was maximally expressed only at high nonphysiological concentrations.

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Year:  1986        PMID： 3542240     DOI： 10.1016/0309-1651(86)90112-8

Source DB:  PubMed          Journal:  Cell Biol Int Rep        ISSN： 0309-1651


  6 in total

1.  Comparative growth of normal and malignant mouse mammary epithelium cultured serum-free on a biomatrix from preadipocytes.

Authors:  N W Shappell; R O Lazo; B B Asch
Journal:  In Vitro Cell Dev Biol       Date:  1991-07

2.  Bovine mammary explant versus primary cell cultures: effect of bovine somatotropin and insulin-like growth factor-I on DNA content and protein synthesis.

Authors:  J E Keys; E Cifrian; A J Guidry; H M Farrell
Journal:  In Vitro Cell Dev Biol Anim       Date:  1997-03       Impact factor: 2.416

3.  In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow.

Authors:  Han Hu; Jiaqi Wang; Dengpan Bu; Hongyang Wei; Linyun Zhou; Fadi Li; Juan J Loor
Journal:  PLoS One       Date:  2009-11-03       Impact factor: 3.240

4.  Establishment and characterization of bovine mammary epithelial cell lines.

Authors:  C A Gibson; J R Vega; C R Baumrucker; C S Oakley; C W Welsch
Journal:  In Vitro Cell Dev Biol       Date:  1991-07

5.  Extracellular matrix and mouse mammary cell function: Comparison of substrata in culture.

Authors:  W L Hurley; D R Blatchford; K A Hendry; C J Wilde
Journal:  In Vitro Cell Dev Biol Anim       Date:  1994-08       Impact factor: 2.416

6.  In vitro culture and evaluation of bovine mammary epithelial cells from Ukraine dairy cows.

Authors:  P Xu; H Fotina; T Fotina; S Wang
Journal:  Iran J Vet Res       Date:  2021       Impact factor: 1.376
  6 in total

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