P Xu1,2,3, H Fotina2, T Fotina2, S Wang1. 1. Department of Veterinary Expertise, College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, 453003 Xinxiang, China. 2. Department of Veterinary Expertise, Microbiology, Zoohygiene and Safety and Quality of Animals' Products, Faculty of Veterinary Medicine, Sumy National Agrarian University, 40021 Sumy, Ukraine. 3. Department of Biotechnology, School of Life Science and Basic Medicine, Xinxiang University, 453003 Xinxiang, China.
Abstract
BACKGROUND: Mammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease. AIMS: This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from the milk of Ukraine Holstein dairy cows in order to develop a general in vitro model. METHODS: The milk from a three-year-old lactating dairy cow was used as a source of the epithelial cell, characteristics of BMECs were examined using real time cell assay (RTCA), immunocytochemistry (ICC), reverse transcription-polymerase chain reaction (RT-PCR), and Western blot (WB). RESULTS: The results showed that BMECs can be recovered from milk, grown in culture, and exhibit the characteristic cobblestone morphology of epithelial cells. CONCLUSION: The established BMECs retained MEC characteristics and secreted β-caseins even when grew on plastic substratum. Thus, the established cell line had normal morphology, growth characteristics, as well as secretory characteristics, and it could be considered as a model system and useful tool for understanding the biology of dairy cow mammary glands.
BACKGROUND: Mammary epithelial cells (MECs) have been widely-used over the years as models to understand the physiological function of mammary disease. AIMS: This study aimed to establish a culture system and elucidate the unique characteristics of bovine mammary epithelial cells (BMECs) from the milk of Ukraine Holstein dairy cows in order to develop a general in vitro model. METHODS: The milk from a three-year-old lactating dairy cow was used as a source of the epithelial cell, characteristics of BMECs were examined using real time cell assay (RTCA), immunocytochemistry (ICC), reverse transcription-polymerase chain reaction (RT-PCR), and Western blot (WB). RESULTS: The results showed that BMECs can be recovered from milk, grown in culture, and exhibit the characteristic cobblestone morphology of epithelial cells. CONCLUSION: The established BMECs retained MEC characteristics and secreted β-caseins even when grew on plastic substratum. Thus, the established cell line had normal morphology, growth characteristics, as well as secretory characteristics, and it could be considered as a model system and useful tool for understanding the biology of dairy cow mammary glands.